公开(公告)号：WO2007133183A3

公开(公告)日：2008-09-12

申请号：PCT/US2006015913

申请日：2006-04-27

Applicant: CARGILL INC ,  BRAZEAU BRIAN J ,  BURKE ELLEN ,  DE SOUZA MERVYN ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMAN SHERRY R ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

Inventor： BRAZEAU BRIAN J ,  BURKE ELLEN ,  DE SOUZA MERVYN ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMAN SHERRY R ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

IPC: C12N9/10 ,  A23L27/20 ,  C07H21/04 ,  C12P13/22

CPC classification number: C12P17/10 ,  C12N9/1096 ,  C12N9/90

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸，一种在某些monatin合成途径（包括一些生物合成途径）中形成的中间体。

公开(公告)号：WO2007133184A3

公开(公告)日：2008-08-28

申请号：PCT/US2006015930

申请日：2006-04-26

Applicant: CARGILL INC ,  BRAZEAU BRIAN J ,  BURKE ELLEN ,  DE SOUZA MERVYN ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMAN SHERRY R ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

Inventor： BRAZEAU BRIAN J ,  BURKE ELLEN ,  DE SOUZA MERVYN ,  GORT STEVEN J ,  HICKS PAULA M ,  KOLLMAN SHERRY R ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SANCHEZ-RIERA FERNANDO A ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

IPC: C12N9/10 ,  C12N1/21 ,  C12P13/22

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

公开(公告)号：WO2007103389A3

公开(公告)日：2008-01-03

申请号：PCT/US2007005718

申请日：2007-03-07

Applicant: CARGILL INC ,  BURKE ELLEN ,  GORT STEVEN JOHN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

Inventor： BURKE ELLEN ,  GORT STEVEN JOHN ,  HICKS PAULA M ,  LUGINBUHL PETER ,  MCFARLAN SARA C ,  RICHARDSON TOBY ,  SOLHEID CHRISTOPHER ,  WEINER DAVID ,  ZHAO LISHAN

IPC: C12P17/00 ,  A23L27/00 ,  A23L27/30 ,  C07D209/20 ,  C12N9/88

CPC classification number: C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的一些立体异构体，例如R3R和S5R构型及其盐。

公开(公告)号：WO2007103989A2

公开(公告)日：2007-09-13

申请号：PCT/US2007/063515

申请日：2007-03-07

Applicant: VERENIUM CORPORATION ,  BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

Inventor： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC: C12N9/10

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

公开(公告)号：WO2006116487A2

公开(公告)日：2006-11-02

申请号：PCT/US2006/015790

申请日：2006-04-26

Applicant: CARGILL, INCORPORATED ,  HICKS, Paula, M. ,  MCFARLAN, Sara, C.

Inventor： HICKS, Paula, M. ,  MCFARLAN, Sara, C.

IPC: C12P13/22 ,  C12N1/18

CPC classification number: C12P17/10 ,  C12N15/52

Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Abstract translation: 提供可用于从葡萄糖，色氨酸，吲哚-3-乳酸，吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸制备莫纳甜的方法和组合物 。 还公开了制备吲哚-3-丙酮酸和2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸中间体的方法。 提供的组合物包括核酸分子，多肽，化学结构和细胞。 方法包括体外和体内过程，体外方法包括化学反应。

公开(公告)号：WO2006066072A2

公开(公告)日：2006-06-22

申请号：PCT/US2005/045575

申请日：2005-12-16

Applicant: CARGILL, INCORPORATED ,  BRAZEAU, Brian ,  DE TROOSTEMBERGH, Jean-Claude ,  HICKS, Paula, M. ,  JESSEN, Holly ,  MCFARLAN, Sara, C. ,  OBYN, Willy ,  SANCHEZ-RIERA, Fernando, A. ,  SCHROEDER, William, A.

Inventor： BRAZEAU, Brian ,  DE TROOSTEMBERGH, Jean-Claude ,  HICKS, Paula, M. ,  JESSEN, Holly ,  MCFARLAN, Sara, C. ,  OBYN, Willy ,  SANCHEZ-RIERA, Fernando, A. ,  SCHROEDER, William, A.

IPC: C12P21/06 ,  C12N9/02 ,  C12N1/21

CPC classification number: C12P7/58 ,  C12P17/181 ,  C12P19/02

Abstract: A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono- -lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono- -lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

Abstract translation: 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了D-葡萄糖醛酸和葡萄糖醛酸内酯的制备方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400克 D-葡萄糖醛酸每升混合物。 葡萄糖醛酸内酯可以由D-葡萄糖醛酸产物生产。 还公开了适用于这种方法的生物体和核酸。

公开(公告)号：WO2005014839A2

公开(公告)日：2005-02-17

申请号：PCT/US2004/024886

申请日：2004-08-02

Applicant: CARGILL, INC. ,  HICKS, Paula, M.

Inventor： HICKS, Paula, M.

IPC: C12P

CPC classification number: A23C9/1544 ,  A21D13/80 ,  A23F3/30 ,  A23G1/56 ,  A23L2/60 ,  A23L27/31 ,  A23L27/32 ,  A23G2200/06

Abstract: The present invention relates to novel sweetener compositions comprising monatin and methods for making such compositions. The present invention also relates to sweetener compositions comprising specific monatin stereoisomers, specific blends of monatin stereoisomers, and /or monatin produced via a biosynthetic pathway in vivo (e.g., inside cells) or in vitro .

Abstract translation: 本发明涉及包含莫纳汀的新型甜味剂组合物以及制备该组合物的方法。 本发明还涉及包含特定莫纳甜立体异构体，莫纳甜立体异构体的特定共混物和/或体内通过生物合成途径（例如细胞内）或体外产生的莫纳甜的甜味剂组合物， / I>

公开(公告)号：WO2002074926A2

公开(公告)日：2002-09-26

申请号：PCT/US2002/008404

申请日：2002-03-19

Applicant: CARGILL INCORPORATED ,  SCHROEDER, William, A. ,  HICKS, Paula M. ,  MCFARLAN Sara C. ,  ABRAHAM, Timothy W.

Inventor： SCHROEDER, William, A. ,  HICKS, Paula M. ,  MCFARLAN Sara C. ,  ABRAHAM, Timothy W.

IPC: C12N

CPC classification number: C12N9/0069 ,  C12N15/52 ,  C12P17/04

Abstract: The invention provides methods and materials related to the production of organic products such as glucuronic acid, ascorbic acid, and glucaric acid. Specifically, the invention provides cells, methods for culturing cells, isolated nucleic acid molecules, and methods and materials for producing various organic products such as glucuronic acid, ascorbic acid, and glucaric acid.

Abstract translation: 本发明提供了与生产有机产物如葡萄糖醛酸，抗坏血酸和葡糖二酸有关的方法和材料。 具体地，本发明提供细胞，培养细胞的方法，分离的核酸分子，以及用于生产各种有机产物如葡萄糖醛酸，抗坏血酸和葡糖二酸的方法和材料。

公开(公告)号：WO2011002469A1

公开(公告)日：2011-01-06

申请号：PCT/US2009/049599

申请日：2009-07-02

Applicant: CARGILL, INCORPORATED ,  DE SOUZA, Mervyn L. ,  NIU, Wei ,  LAPLAZA, Jose ,  SOLDHEID, Christopher ,  KOLLMANN, Sherry R. ,  LUNDORFF, Joshua M. ,  HICKS, Paula M. ,  MCFARLAN, Sara C. ,  SANCHEZ-RIERA, Fernando A. ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

Inventor： DE SOUZA, Mervyn L. ,  NIU, Wei ,  LAPLAZA, Jose ,  SOLDHEID, Christopher ,  KOLLMANN, Sherry R. ,  LUNDORFF, Joshua M. ,  HICKS, Paula M. ,  MCFARLAN, Sara C. ,  SANCHEZ-RIERA, Fernando A. ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

IPC: C12P7/40 ,  C12N15/00

CPC classification number: C12N9/90 ,  C12P13/06 ,  C12P13/22 ,  C12P17/10

Abstract: The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.

Abstract translation: 本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。 还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

公开(公告)号：WO2009088482A1

公开(公告)日：2009-07-16

申请号：PCT/US2008/014137

申请日：2008-12-31

Applicant: CARGILL, INCORPORATED ,  DE SOUZA, Mervyn L. ,  HICKS, Paula M. ,  KOLLMANN, Sherry R. ,  LAPLAZA, José M. ,  LUNDORFF, Joshua M. ,  MCFARLAN, Sara C. ,  MARASCO, Erin ,  NIU, Wei ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

Inventor： DE SOUZA, Mervyn L. ,  HICKS, Paula M. ,  KOLLMANN, Sherry R. ,  LAPLAZA, José M. ,  LUNDORFF, Joshua M. ,  MCFARLAN, Sara C. ,  MARASCO, Erin ,  NIU, Wei ,  SANCHEZ-RIERA, Fernando A. ,  SOLHEID, Christopher ,  WEINER, David P. ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin

IPC: C12P21/06 ,  C12N9/10

CPC classification number: C12P13/227 ,  C12N9/0004 ,  C12N9/1096 ,  C12P17/10

Abstract: The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

Abstract translation: 本发明提供氨基转移酶和氧化还原酶多肽以及编码这种多肽的核酸。 还提供了使用这种氨基转移酶和氧化还原酶核酸和多肽的方法。