公开(公告)号：WO2005017499A2

公开(公告)日：2005-02-24

申请号：PCT/US2004/026225

申请日：2004-08-11

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D. ,  MOORE, Douglas, E.

Inventor： ROTH, Wayne, D. ,  MOORE, Douglas, E.

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪类型测量系统的一个或多个参数的各种方法。 一个实施例包括在测量样本微球体期间监测测量系统的参数。 该方法还包括基于监测实时改变参数。 另一种方法包括监测接近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体的温度，其中设置有样本微球体。 该方法还包括根据温度下流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：WO2008061058A3

公开(公告)日：2008-07-03

申请号：PCT/US2007084417

申请日：2007-11-12

Applicant: LUMINEX CORP ,  KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

Inventor： KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

公开(公告)号：WO2007035840B1

公开(公告)日：2007-12-06

申请号：PCT/US2006036733

申请日：2006-09-21

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

Abstract translation: 提供了用于图像数据处理的方法，存储介质和系统。 用于该方法，存储介质和系统的实施例包括执行以下步骤中的一个或多个的配置：背景信号测量，使用分类染料发射和簇拒绝的粒子识别，图像间对齐，图像间粒子相关， 记者发射和图像平面归一化。

公开(公告)号：WO2005017498B1

公开(公告)日：2006-06-01

申请号：PCT/US2004024987

申请日：2004-08-03

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1434

Abstract: Various light emitting diode (LED) based measurement systems and methods are provided. One system includes one or more arrays of LEDs (14, 16, 18) arranged along a flow path (20) of a sample (10). The array(s) are configured to illuminate the sample as the sample moves along the flow path. The system also includes one or more detectors (24) configured to detect light resulting from illumination of the sample by the array(s). One method includes illuminating a microsphere at different positions along a flow path of the microsphere. The method also includes detecting light resulting from the illumination to produce individual output signals corresponding to the illumination at the different positions. The method further includes combining the individual OUTPUT signals to produce a single output signal having a signal-to-noise ratio that is greater than a signal-to-noise ratio of the individual output signals.

Abstract translation: 提供了各种基于发光二极管（LED）的测量系统和方法。 一个系统包括沿着样品（10）的流动路径（20）布置的一个或多个LED阵列（14,16,18）。 阵列被配置为当样品沿着流动路径移动时照亮样品。 该系统还包括一个或多个检测器（24），其被配置为检测由阵列照射样品产生的光。 一种方法包括沿着微球的流动路径在不同位置照射微球。 该方法还包括检测由照明产生的光以产生对应于不同位置处的照明的各个输出信号。 该方法还包括组合各个OUTPUT信号以产生具有大于各个输出信号的信噪比的信噪比的单个输出信号。

公开(公告)号：WO2005017499A3

公开(公告)日：2005-08-18

申请号：PCT/US2004026225

申请日：2004-08-13

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  MOORE DOUGLAS E

Inventor： ROTH WAYNE D ,  MOORE DOUGLAS E

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：WO2008061058A2

公开(公告)日：2008-05-22

申请号：PCT/US2007/084417

申请日：2007-11-12

Applicant: LUMINEX CORPORATION ,  KRAGER, Jarden, E. ,  DEICHER, William, R. ,  SCHILFFARTH, Adam, Richard ,  PEMPSELL, Paul ,  ROTH, Wayne, D.

Inventor： KRAGER, Jarden, E. ,  DEICHER, William, R. ,  SCHILFFARTH, Adam, Richard ,  PEMPSELL, Paul ,  ROTH, Wayne, D.

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

Abstract translation: 提供了流式细胞仪，其包括询问流动池和延伸到询问流动池中的多个测定流体线。 操作这种流式细胞计数器的方法包括用鞘液灌注询问流动池，并将不同的分析流体注入通过多条流体管线的鞘液流。 提供流体线组件，其包括多个毛细管，所述多个毛细管耦合到构造成耦合到流式细胞仪的询问流动池组件的基部。 毛细管的尺寸构造使得当流体线组件布置在流式细胞计数器内并且流体从一个或多个毛细管以相对于询问流动池内的包围护套流体的给定压力差分配时，流体 在询问流动池内基本集中对齐。

公开(公告)号：WO2007035840A3

公开(公告)日：2007-10-04

申请号：PCT/US2006036733

申请日：2006-09-21

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

Abstract translation: 提供了用于图像数据处理的方法，存储介质和系统。 用于方法，存储介质和系统的实施例包括执行以下一个或多个步骤的配置：背景信号测量，使用分类染料发射和簇排斥的粒子识别，图像间对准，图像间粒子相关，荧光整合 记者发射和图像平面归一化。

公开(公告)号：WO2007016517B1

公开(公告)日：2007-04-05

申请号：PCT/US2006029806

申请日：2006-08-01

Applicant: LUMINEX CORP ,  CALVIN EDWARD A ,  ROTH WAYNE D

Inventor： CALVIN EDWARD A ,  ROTH WAYNE D

IPC: G01N15/14 ,  G06F19/00

CPC classification number: G01N15/1456 ,  G01N2015/1477 ,  G01N2015/1488 ,  G06F19/707

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract translation: 提供了用于分类粒子的方法，数据结构和系统。 具体而言，所述方法和系统被配置为获取与微粒的可测量参数相对应的第一组数据，并识别与第一组数据对应的查找表的位置，其中查找表被构建 通过与至少一个可测量参数相关联的值。 此外，方法和系统被配置为确定第一组数据是否适合分别指示与查找表的识别位置相关联的不同微粒分类的一个或多个预定义算法。 所述方法和系统进一步被配置为基于确定所述第一组数据是否适合所述一个或多个预定义算法来将所述微粒分类在至少一个预定义分类内。

公开(公告)号：WO2005017498A1

公开(公告)日：2005-02-24

申请号：PCT/US2004/024987

申请日：2004-08-03

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D.

Inventor： ROTH, Wayne, D.

IPC: G01N15/14

CPC classification number: G01N15/1434

Abstract: Various light emitting diode (LED) based measurement systems and methods are provided. One system includes one or more arrays of LEDs (14, 16, 18) arranged along a flow path (20) of a sample (10). The array(s) are configured to illuminate the sample as the sample moves along the flow path. The system also includes one or more detectors (24) configured to detect light resulting from illumination of the sample by the array(s). One method includes illuminating a microsphere at different positions along a flow path of the microsphere. The method also includes detecting light resulting from the illumination to produce individual output signals corresponding to the illumination at the different positions. The method further includes combining the individual OUTPUT signals to produce a single output signal having a signal-to-noise ratio that is greater than a signal-to-noise ratio of the individual output signals.

Abstract translation: 提供了各种基于发光二极管（LED）的测量系统和方法。 一个系统包括沿着样品（10）的流动路径（20）布置的一个或多个LED阵列（14,16,18）。 阵列被配置为当样品沿着流动路径移动时照亮样品。 该系统还包括一个或多个检测器（24），其被配置为检测由阵列照射样品产生的光。 一种方法包括沿着微球的流动路径在不同位置照射微球。 该方法还包括检测由照明产生的光以产生对应于不同位置处的照明的各个输出信号。 该方法还包括组合各个OUTPUT信号以产生具有大于各个输出信号的信噪比的信噪比的单个输出信号。