公开(公告)号：WO2019115769A1

公开(公告)日：2019-06-20

申请号：PCT/EP2018/084965

申请日：2018-12-14

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： MEINJOHANNS, Ernst ,  HARLOW, Kenneth

IPC: B01D15/18 ,  B01D15/38 ,  A23C9/14 ,  A23C7/04 ,  B01D15/30

CPC classification number: B01D15/1807 ,  A23C9/14 ,  B01D15/305 ,  B01D15/3804 ,  B01D15/3828 ,  B01D15/3847

Abstract: The present invention relates to a method for separating one or more oligosaccharide compound(s) from a preliminary dairy source, the method comprises the steps of: (i) Providing the preliminary dairy source; (ii) Contacting the preliminary dairy source with a chromatographic support allowing one or more oligosaccharide compound(s) present in the preliminary dairy source to be retained by the chromatographic support; (iii) Obtaining a unretained, flow through fraction from the chromatographic support comprising a secondary dairy source; (iv) Optionally washing the chromatographic support; and (v) Subjecting the chromatographic support to at least one elution buffer obtaining one or more oligosaccharide compound(s) from the chromatographic support; wherein the chromatographic support comprises one or more adsorbent capable of specifically binding the one or more oligosaccharide compound(s) from the preliminary dairy source.

公开(公告)号：WO2010037736A1

公开(公告)日：2010-04-08

申请号：PCT/EP2009/062602

申请日：2009-09-29

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

Inventor： HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

IPC: A23C9/146 ,  A23C21/00 ,  A23J1/20

CPC classification number: A23L1/3056 ,  A23C21/06 ,  A23J1/205 ,  A23L33/19 ,  A23V2002/00 ,  C07K1/18 ,  A23V2250/54252 ,  A23V2250/54242 ,  A23V2250/54244

Abstract: The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a β-lactoglobulin product and the isolation of an α-enriched whey protein isolate from whey obtained from an animal. The α-enriched whey protein isolate provided by the present invention is besides from being low in β- lactoglobulin also high in α-lactalbumin and immunoglobulin G.

Abstract translation: 本发明涉及乳清蛋白的分离和乳清产物和乳清分离物的制备。 特别地，本发明涉及分离β-乳球蛋白产物和从动物获得的乳清中分离富含α-的乳清蛋白分离物。 本发明提供的α-富集的乳清蛋白分离物除了β-乳球蛋白低，α-乳白蛋白和免疫球蛋白G也高。

公开(公告)号：WO2008092450A1

公开(公告)日：2008-08-07

申请号：PCT/DK2008/000039

申请日：2008-01-29

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  PONTOPPIDAN, Martin

Inventor： LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  PONTOPPIDAN, Martin

IPC: C07K14/81 ,  C07K14/415

CPC classification number: C07K14/81 ,  C07K1/22 ,  C07K14/415

Abstract: The present invention relates to the large-scale fractionation and isolation of peptides, polypeptides and protein(s) from a potato derived protein solution such as potato extract, potato fruit juice and fruit water using an adsorbent coupled with a ligand for the capture of the protein(s), from the protein solution. In particular the invention relates to a process for the isolation and separation of patatin and potato protease inhibitors using a low temperature non-denaturing process.

Abstract translation: 本发明涉及使用与配体偶联的吸附剂从马铃薯衍生的蛋白质溶液如马铃薯提取物，马铃薯果汁和水果水中大量分级分离和分离肽，多肽和蛋白质，以捕获 蛋白质，从蛋白质溶液中。 特别地，本发明涉及使用低温非变性方法分离和分离patatin和马铃薯蛋白酶抑制剂的方法。

公开(公告)号：WO2004008138A2

公开(公告)日：2004-01-22

申请号：PCT/DK2003/000483

申请日：2003-07-09

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan, Otto, Fog ,  HEEGAARD, Peter, M., H.

Inventor： LIHME, Allan, Otto, Fog ,  HEEGAARD, Peter, M., H.

IPC: G01N33/50

CPC classification number: B01D15/3804 ,  A61M1/3681 ,  B01D15/1807 ,  B01J20/262 ,  B01J20/264 ,  B01J20/267 ,  B01J20/28019 ,  B01J20/28061 ,  B01J20/286 ,  B01J20/3092 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/56 ,  B01J2220/58

Abstract: The present invention provides an extracorporeal adsorption method for removing harmful substances from blood in a way that is practicable in everyday clinical practice and applicable for the timely intervention to present the development of sepsis. Said extracorporeal adsorption method being effected by an adsorption column assembly where the adsorption column assembly comprising a column and an adsorption medium in the form of particles. The sedimented volume of said particles being at the most 80% of the volume of the column.

Abstract translation: 本发明提供了一种用于从日常临床实践中切实可行的适用于呈现脓毒症发展的方法从血液中除去有害物质的体外吸附方法。 所述体外吸附方法由吸附柱组件实现，其中吸附柱组件包括柱状物和颗粒形式的吸附介质。 所述颗粒的沉积体积为柱体积的最大80％。

公开(公告)号：WO2002096215A1

公开(公告)日：2002-12-05

申请号：PCT/DK2002/000375

申请日：2002-05-31

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  ANDERSEN, Inga, Vaarst ,  OLANDER, Morten, Aae

Inventor： LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  ANDERSEN, Inga, Vaarst ,  OLANDER, Morten, Aae

IPC: A23J1/20

CPC classification number: B01D15/1807 ,  A23J1/00 ,  A23J1/20 ,  A23J1/205 ,  A23V2002/00 ,  B01D15/1871 ,  B01D15/265 ,  B01D15/424 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/286 ,  B01J20/3268 ,  B01J20/3293 ,  A23V2250/54242 ,  A23V2250/54244 ,  A23V2250/54248 ,  A23V2250/5425 ,  A23V2250/5434 ,  A23V2300/30

Abstract: Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture, b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 μm, c) optionally washing the column, d) eluting at least one protein from the adsorbent.

Abstract translation: 因此，本发明的主要方面涉及一种含蛋白质混合物的分级方法，其中含蛋白质的混合物选自牛奶，奶衍生产品，乳衍生原料，植物衍生产品， 所述方法包括以下步骤：a）任选地调节混合物的pH值，b）将所述混合物施加到包含吸附剂的吸附塔上 所述吸附剂包括具有至少一个高密度无孔芯的颗粒，被多孔材料包围，所述吸附剂具有至少1.5g / ml的颗粒密度和至多150μm的平均粒度，c） 任选地洗涤柱，d）从吸附剂中洗脱出至少一种蛋白质。

公开(公告)号：WO2016055064A2

公开(公告)日：2016-04-14

申请号：PCT/DK2015/000039

申请日：2015-10-06

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix

IPC: A23C9/146

CPC classification number: A23J1/20 ,  A23C9/1465 ,  A23C19/02 ,  A61K8/64 ,  A61K8/986 ,  A61K2800/10 ,  A61Q19/00 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/165 ,  C07K14/47

Abstract: The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.

Abstract translation: 本发明涉及从聚集的含酪蛋白的材料中分离至少一种可溶性蛋白组分的方法，所述方法包括以下步骤：（i）提供聚集的含酪蛋白的材料; （ii）将聚集的含酪蛋白的材料与色谱载体接触，使得存在于聚集的含酪蛋白的材料中的一种或多种可溶蛋白被色谱载体保留; （iii）从包含聚集的酪蛋白的层析载体获得渗透物部分; （iv）任选洗涤色谱载体; （v）使色谱载体经历至少一种洗脱缓冲液，从色谱载体获得至少一种可溶性蛋白质部分; 并且其中色谱载体包含能够从聚集的含酪蛋白的材料中结合可溶性蛋白质的一种或多种混合模式配体。

公开(公告)号：WO2016023553A1

公开(公告)日：2016-02-18

申请号：PCT/DK2015/000033

申请日：2015-08-14

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan, Otto, Fog. ,  HANSEN, Marie Bendix

IPC: A23C9/146 ,  A23J1/20 ,  C07K14/47

CPC classification number: A23J1/205 ,  A23C9/1465 ,  C07K1/18 ,  C07K14/4717 ,  C07K14/76

Abstract: The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.

Abstract translation: 本发明涉及一种从乳中获得的乳清材料提供α-乳白蛋白部分和β-乳球蛋白部分的方法，该方法包括以下步骤：（i）提供乳清材料; （ii）使乳清物质与色谱支持物接触，使得β-乳球蛋白被色谱支持物保留; （iii）从包含α-乳白蛋白部分的色谱支架获得渗透部分; （iv）任选地洗涤色谱支架; 和（v）从包含β-乳球蛋白级分的色谱载体获得滞留物级分; 其中步骤（i）中提供的乳清材料已经从至少一种乳清蛋白（例如至少2个乳清蛋白，例如至少一种）消耗或基本上消耗。 至少3种乳清蛋白。

公开(公告)号：WO2011161261A1

公开(公告)日：2011-12-29

申请号：PCT/EP2011/060667

申请日：2011-06-24

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan Otto Fog ,  STANLEY, Christopher John

Inventor： LIHME, Allan Otto Fog ,  STANLEY, Christopher John

IPC: C12P7/02 ,  C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  C12P7/16 ,  C12P7/62 ,  C12P7/64

CPC classification number: C12P7/10 ,  B01D15/168 ,  B01D15/1892 ,  B01D15/3809 ,  C07K1/22 ,  C12P7/04 ,  C12P7/16 ,  C12P7/649 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/17

Abstract: A method of conducting an enzymatic process in which the enzymes are recovered and reused in at least a second iteration of the process, wherein each iteration of the process comprises the steps of: (a) providing a heterogeneous substrate solution, in which the substrate is fully soluble, partially soluble or insoluble; (b) adding an enzyme or mixture of enzymes to the heterogeneous substrate solution; and (c) allowing an enzymatic reaction of the substrate to proceed; wherein, after completion of step (c) for each iteration, the enzyme is recovered from the mixture resulting from step (c) according to the following steps: (d) conducting a non-packed-bed adsorption process comprising contacting the reaction mixture with an adsorbent that adsorbs the enzyme in order to separate the enzyme from the reaction mixture resulting from step (c); (e) optionally washing unbound material from the adsorbent; and (f) desorbing the enzyme from the adsorbent; and further wherein the desorbed enzyme obtained in step (f) is used in step (b) of at least one subsequent iteration of the process.

Abstract translation: 一种进行酶过程的方法，其中酶在该方法的至少第二次迭代中回收和重复使用，其中该方法的每次迭代包括以下步骤：（a）提供非均相底物溶液，其中所述底物为 完全溶解，部分溶解或不溶; （b）向所述非均相底物溶液中加入酶或酶的混合物; 和（c）允许底物的酶促反应进行; 其中，对于每次迭代，在步骤（c）完成后，根据以下步骤从步骤（c）得到的混合物中回收酶：（d）进行非填充床吸附方法，包括使反应混合物与 吸附酶以便将酶与步骤（c）得到的反应混合物分离的吸附剂; （e）任选地从吸附剂洗涤未结合的材料; 和（f）从吸附剂中解吸酶; 并且其中步骤（f）中获得的解吸酶在步骤（b）中用于该方法的至少一次后续迭代。

公开(公告)号：WO2004082397A1

公开(公告)日：2004-09-30

申请号：PCT/DK2004/000187

申请日：2004-03-19

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  HANSEN, Marie, Bendix ,  LIHME, Allan, Otto, Fog

Inventor： HANSEN, Marie, Bendix ,  LIHME, Allan, Otto, Fog

IPC: A23J1/20

CPC classification number: C07K1/16 ,  A23J1/005 ,  A23J1/08 ,  A23J1/20 ,  A23J1/205 ,  B01D15/12 ,  B01D15/18 ,  B01D15/1807

Abstract: The present invention provides industrial scale expanded bed adsorption process for fractionation and isolation of bio-molecules from fluids, preferably proteins from milk and whey, in a cost-effective manner. This is accomplished by operating the expanded bed column at high temperatures of at last 40°C, combined with applying flow rates greater than 1.500 cm/hour.

Abstract translation: 本发明提供工业规模的膨胀床吸附方法，用于以成本有效的方式从生物分子与流体，优选来自乳和乳清的蛋白质分离和分离。 这是通过在最后40℃的高温下操作膨胀床柱，结合施加大于1.500cm /小时的流速来实现的。

公开(公告)号：US20150299275A1

公开(公告)日：2015-10-22

申请号：US14437630

申请日：2013-10-23

Applicant: Upfront Chromatography A/S

Inventor： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Martin Pontoppidan

IPC: C07K14/415 ,  C07K1/113

CPC classification number: C07K1/22 ,  A23J1/14 ,  C07K1/1136 ,  C07K1/14 ,  C07K14/415 ,  C08L89/00

Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.

Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于水提取物或大豆蛋白溶液，其通过至少一个膨胀床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。