公开(公告)号：WO2016055064A2

公开(公告)日：2016-04-14

申请号：PCT/DK2015/000039

申请日：2015-10-06

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix

IPC: A23C9/146

CPC classification number: A23J1/20 ,  A23C9/1465 ,  A23C19/02 ,  A61K8/64 ,  A61K8/986 ,  A61K2800/10 ,  A61Q19/00 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/165 ,  C07K14/47

Abstract: The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.

Abstract translation: 本发明涉及从聚集的含酪蛋白的材料中分离至少一种可溶性蛋白组分的方法，所述方法包括以下步骤：（i）提供聚集的含酪蛋白的材料; （ii）将聚集的含酪蛋白的材料与色谱载体接触，使得存在于聚集的含酪蛋白的材料中的一种或多种可溶蛋白被色谱载体保留; （iii）从包含聚集的酪蛋白的层析载体获得渗透物部分; （iv）任选洗涤色谱载体; （v）使色谱载体经历至少一种洗脱缓冲液，从色谱载体获得至少一种可溶性蛋白质部分; 并且其中色谱载体包含能够从聚集的含酪蛋白的材料中结合可溶性蛋白质的一种或多种混合模式配体。

公开(公告)号：WO2016023553A1

公开(公告)日：2016-02-18

申请号：PCT/DK2015/000033

申请日：2015-08-14

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan, Otto, Fog. ,  HANSEN, Marie Bendix

IPC: A23C9/146 ,  A23J1/20 ,  C07K14/47

CPC classification number: A23J1/205 ,  A23C9/1465 ,  C07K1/18 ,  C07K14/4717 ,  C07K14/76

Abstract: The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.

Abstract translation: 本发明涉及一种从乳中获得的乳清材料提供α-乳白蛋白部分和β-乳球蛋白部分的方法，该方法包括以下步骤：（i）提供乳清材料; （ii）使乳清物质与色谱支持物接触，使得β-乳球蛋白被色谱支持物保留; （iii）从包含α-乳白蛋白部分的色谱支架获得渗透部分; （iv）任选地洗涤色谱支架; 和（v）从包含β-乳球蛋白级分的色谱载体获得滞留物级分; 其中步骤（i）中提供的乳清材料已经从至少一种乳清蛋白（例如至少2个乳清蛋白，例如至少一种）消耗或基本上消耗。 至少3种乳清蛋白。

公开(公告)号：WO2014064132A1

公开(公告)日：2014-05-01

申请号：PCT/EP2013/072128

申请日：2013-10-23

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  PONTOPPIDAN, Martin

IPC: C07K1/22 ,  C07K14/415

CPC classification number: C07K1/22 ,  A23J1/14 ,  C07K1/1136 ,  C07K1/14 ,  C07K14/415 ,  C08L89/00

Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.

Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于水提取物或大豆蛋白溶液，其通过至少一个膨胀床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。

公开(公告)号：WO2009071560A1

公开(公告)日：2009-06-11

申请号：PCT/EP2008/066659

申请日：2008-12-02

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  HOLTE, René Oehlenschlaeger ,  LARSEN, Brian

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  HOLTE, René Oehlenschlaeger ,  LARSEN, Brian

IPC: B01J13/04

CPC classification number: B01J2/08 ,  B01J13/046

Abstract: The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilising the droplets by transferring the emulsion from the first reactor to a stabilisation reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.

Abstract translation: 本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）将（a）包含能够凝胶化的材料的液体组合物组合; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够 凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经历凝胶化手段以在5分钟或更短时间内获得凝胶化，并形成珠粒。

公开(公告)号：WO2010037736A1

公开(公告)日：2010-04-08

申请号：PCT/EP2009/062602

申请日：2009-09-29

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

Inventor： HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

IPC: A23C9/146 ,  A23C21/00 ,  A23J1/20

CPC classification number: A23L1/3056 ,  A23C21/06 ,  A23J1/205 ,  A23L33/19 ,  A23V2002/00 ,  C07K1/18 ,  A23V2250/54252 ,  A23V2250/54242 ,  A23V2250/54244

Abstract: The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a β-lactoglobulin product and the isolation of an α-enriched whey protein isolate from whey obtained from an animal. The α-enriched whey protein isolate provided by the present invention is besides from being low in β- lactoglobulin also high in α-lactalbumin and immunoglobulin G.

Abstract translation: 本发明涉及乳清蛋白的分离和乳清产物和乳清分离物的制备。 特别地，本发明涉及分离β-乳球蛋白产物和从动物获得的乳清中分离富含α-的乳清蛋白分离物。 本发明提供的α-富集的乳清蛋白分离物除了β-乳球蛋白低，α-乳白蛋白和免疫球蛋白G也高。

公开(公告)号：EP2912052A1

公开(公告)日：2015-09-02

申请号：EP13792269.6

申请日：2013-10-23

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  PONTOPPIDAN, Martin

IPC: C07K1/22 ,  C07K14/415

CPC classification number: C07K1/22 ,  A23J1/14 ,  C07K1/1136 ,  C07K1/14 ,  C07K14/415 ,  C08L89/00

Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.

Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于大豆蛋白质的水提取物或溶液，其通过至少一个扩展床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。

公开(公告)号：EP2355664A1

公开(公告)日：2011-08-17

申请号：EP09783540.9

申请日：2009-09-29

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

IPC: A23C9/146 ,  A23C21/00 ,  A23J1/20

CPC classification number: A23L1/3056 ,  A23C21/06 ,  A23J1/205 ,  A23L33/19 ,  A23V2002/00 ,  C07K1/18 ,  A23V2250/54252 ,  A23V2250/54242 ,  A23V2250/54244

Abstract: The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a β-lactoglobulin product and the isolation of an α-enriched whey protein isolate from whey obtained from an animal. The α-enriched whey protein isolate provided by the present invention is besides from being low in β- lactoglobulin also high in α-lactalbumin and immunoglobulin G.

公开(公告)号：EP2355664B1

公开(公告)日：2014-07-02

申请号：EP09783540.9

申请日：2009-09-29

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

IPC: A23C9/146 ,  A23C21/00 ,  A23J1/20

CPC classification number: A23L1/3056 ,  A23C21/06 ,  A23J1/205 ,  A23L33/19 ,  A23V2002/00 ,  C07K1/18 ,  A23V2250/54252 ,  A23V2250/54242 ,  A23V2250/54244

公开(公告)号：EP2355664B8

公开(公告)日：2014-09-10

申请号：EP09783540.9

申请日：2009-09-29

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： HANSEN, Marie Bendix ,  LIHME, Allan Otto Fog ,  LIHME, Malene Fog

IPC: A23C9/146 ,  A23C21/00 ,  A23J1/20

CPC classification number: A23L1/3056 ,  A23C21/06 ,  A23J1/205 ,  A23L33/19 ,  A23V2002/00 ,  C07K1/18 ,  A23V2250/54252 ,  A23V2250/54242 ,  A23V2250/54244

公开(公告)号：EP3203852A2

公开(公告)日：2017-08-16

申请号：EP15787446.2

申请日：2015-10-06

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix

IPC: A23C9/146 ,  A23J1/20 ,  B01D15/38 ,  C07K16/04 ,  C07K1/16

CPC classification number: A23J1/20 ,  A23C9/1465 ,  A23C19/02 ,  A61K8/64 ,  A61K8/986 ,  A61K2800/10 ,  A61Q19/00 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/165 ,  C07K14/47

Abstract: The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.

Abstract translation: 本发明涉及从聚集的含酪蛋白的材料中分离至少一种可溶性蛋白质部分的方法，所述方法包括以下步骤：（i）提供聚集的含酪蛋白的材料; （ii）将聚集的含酪蛋白的材料与色谱载体接触，使得存在于聚集的含酪蛋白的材料中的一种或多种可溶蛋白被色谱载体保留; （iii）从包含聚集的酪蛋白的层析载体获得渗透物部分; （iv）任选洗涤色谱载体; （v）使色谱载体经历至少一种洗脱缓冲液，从色谱载体获得至少一种可溶性蛋白质部分; 并且其中所述色谱载体包含能够从所述聚集的含酪蛋白的材料结合所述可溶性蛋白的一种或多种混合模式配体。