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    SEPARATION PROCESSES FOR SOY PROTEIN
    3.
    发明申请
    SEPARATION PROCESSES FOR SOY PROTEIN 审中-公开
    大豆蛋白的分离过程

    公开(公告)号：WO2014064132A1

    公开(公告)日：2014-05-01

    申请号：PCT/EP2013/072128

    申请日：2013-10-23

    Applicant: UPFRONT CHROMATOGRAPHY A/S

    Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  PONTOPPIDAN, Martin

    IPC: C07K1/22 ,  C07K14/415

    CPC classification number: C07K1/22 ,  A23J1/14 ,  C07K1/1136 ,  C07K1/14 ,  C07K14/415 ,  C08L89/00

    Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.

    Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于水提取物或大豆蛋白溶液，其通过至少一个膨胀床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。

    SYSTEM AND METHOD FOR PRODUCING BEADS
    4.
    发明申请
    SYSTEM AND METHOD FOR PRODUCING BEADS 审中-公开
    用于生产珠粒的系统和方法

    公开(公告)号：WO2009071560A1

    公开(公告)日：2009-06-11

    申请号：PCT/EP2008/066659

    申请日：2008-12-02

    Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  HOLTE, René Oehlenschlaeger ,  LARSEN, Brian

    Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix ,  HOLTE, René Oehlenschlaeger ,  LARSEN, Brian

    IPC: B01J13/04

    CPC classification number: B01J2/08 ,  B01J13/046

    Abstract: The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilising the droplets by transferring the emulsion from the first reactor to a stabilisation reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.

    Abstract translation: 本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）将（a）包含能够凝胶化的材料的液体组合物组合; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够 凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经历凝胶化手段以在5分钟或更短时间内获得凝胶化，并形成珠粒。

    EXPANDED BED COLUMN AND DISPOSABLE CHROMATOGRAPHY
    6.
    发明申请
    EXPANDED BED COLUMN AND DISPOSABLE CHROMATOGRAPHY 审中-公开
    膨化床柱和可排除的色谱

    公开(公告)号：WO2008116935A1

    公开(公告)日：2008-10-02

    申请号：PCT/EP2008/053732

    申请日：2008-03-28

    Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan ,  HOLTE, René, Oehlenschläger ,  JENSEN, Kurt, Hauge ,  CHRISTENSEN, Tony

    Inventor： LIHME, Allan ,  HOLTE, René, Oehlenschläger ,  JENSEN, Kurt, Hauge ,  CHRISTENSEN, Tony

    IPC: B01D15/18 ,  G01N30/38

    CPC classification number: B01D15/1807 ,  C07K1/22 ,  G01N30/38

    Abstract: In the field of expanded bed adsorption chromatography, with particular but not exclusive relevance to disposable expanded bed chromatography columns, a method of conducting upward flow expanded bed chromatography comprising: supplying a liquid via an inlet to a stationary phase medium contained in a column, allowing adsorption of at least one component from the liquid by the stationary phase medium, withdrawing the liquid from the column via an outlet, regulating the expansion of the stationary phase medium by regulation of the flow rate of the liquid through at least the inlet, and restricting any overpressure in the headspace of the column to not more than the outside pressure plus 0.1 bar, is provided. In addition, apparatus for use in said method, in particular columns for use in expanded bed chromatography, are provided.

    Abstract translation: 在膨胀床吸附色谱领域中，与一次性膨胀床色谱柱特别但不排他地相关，一种向上流扩张床层析的方法，包括：通过入口将液体供给到容纳在柱中的固定相介质，允许 通过固定相介质从液体中吸收至少一种组分，通过出口从柱中排出液体，通过至少通过入口限制液体的流速来调节固定相介质的膨胀，并限制 提供柱的顶部空间中的任何超压力不超过外部压力加上0.1巴。 此外，提供了用于所述方法的设备，特别是用于扩展床层析的色谱柱。

    PRODUCTION OF BIOFUEL AND PROTEIN FROM A RAW MATERIAL
    7.
    发明申请
    PRODUCTION OF BIOFUEL AND PROTEIN FROM A RAW MATERIAL 审中-公开
    从原料生产生物和蛋白质

    公开(公告)号：WO2008086811A1

    公开(公告)日：2008-07-24

    申请号：PCT/DK2008/050007

    申请日：2008-01-15

    Applicant: Upfront Chromatography A/S ,  LIHME, Allan Otto Fog

    Inventor： LIHME, Allan Otto Fog

    IPC: B01D15/02 ,  C12P7/10 ,  C07K1/22 ,  A23J1/00 ,  B01D15/00 ,  C10L1/02 ,  C10L1/18

    CPC classification number: C10L1/1802 ,  B01D15/1807 ,  C07K1/16 ,  C12P3/00 ,  C12P5/023 ,  C12P7/02 ,  C12P7/08 ,  C12P7/16 ,  C12P7/62 ,  C12P7/649 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

    Abstract: The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspensionfrom step (iii) to an expanded bed adsorption process obtaining the purified protein product.

    Abstract translation: 本发明涉及从适合于生产生物燃料的原料或所述原料的衍生物提供分离的生物燃料和纯化的蛋白质产物的方法。 该方法包括以下步骤：（i）使所述原料或所述原料的衍生物经受从原料或所述原料的衍生物释放生物燃料的至少一种第一处理，（ii）将所释放的生物燃料分离 步骤（i）获得分离的生物燃料，（iii）使所述原料或所述原料的衍生物经受提供材料悬浮液的至少一次第二处理，和（iv）使来自步骤（iii）的材料悬浮液经受膨胀床 获得纯化的蛋白质产物的吸附过程。

    ISOLATION OF PEPTIDES, POLYPEPTIDES AND PROTEINS
    8.
    发明申请
    ISOLATION OF PEPTIDES, POLYPEPTIDES AND PROTEINS 审中-公开
    肽，多糖和蛋白质的分离

    公开(公告)号：WO2007063129A2

    公开(公告)日：2007-06-07

    申请号：PCT/EP2006/069208

    申请日：2006-12-01

    Applicant: NOVOZYMES A/S ,  UPFRONT CHROMATOGRAPHY A/S ,  CHRISTENSEN, Bjarke ,  NØRGAARD, Allan

    Inventor： CHRISTENSEN, Bjarke ,  NØRGAARD, Allan

    IPC: C07K14/43

    CPC classification number: C07K14/765 ,  C07K1/20 ,  C07K14/473 ,  C07K14/745 ,  C07K14/8125

    Abstract: The present invention provides a process for the isolation of rHSA from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific protein(s) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more protein(s) from the column.

    Abstract translation: 本发明提供了从蛋白质溶液中分离rHSA的方法。 该方法包括以下步骤：a）提供包含一种或多种特定蛋白质并具有预设pH和预设离子强度或电导率的蛋白质溶液，b）将蛋白质溶液施用于填充床或膨胀床柱，其包含 吸附剂，和c）从所述塔中获得一种或多种蛋白质。

    ISOLATION OF PLASMA OR SERUM PROTEINS
    9.
    发明申请
    ISOLATION OF PLASMA OR SERUM PROTEINS 审中-公开
    血浆或血清蛋白的分离

    公开(公告)号：WO2005121163A2

    公开(公告)日：2005-12-22

    申请号：PCT/DK2005/000378

    申请日：2005-06-07

    Applicant: UPFRONT CHROMATOGRAPHY A/S ,  LIHME, Allan, Otto, Fog

    Inventor： LIHME, Allan, Otto, Fog

    IPC: C07K1/00

    CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

    Abstract: The present invention provides a process for the isolation of one or more proteins) from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific proteins) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been supplemented with an alcohol.

    Abstract translation: 本发明提供了从蛋白质溶液中分离一种或多种蛋白质的方法）。 该方法包括以下步骤：a）提供包含一种或多种特定蛋白质的蛋白质溶液）并具有预设的pH和预设的离子强度或电导率，b）将蛋白质溶液施加到包含吸附剂的填充床或膨胀床柱 ，和c）从所述柱中获得一种或多种蛋白质） 其中所述蛋白质溶液已经被补充了醇。

    SEPARATION OF OLIGOSACCHARIDES
    10.
    发明申请
    SEPARATION OF OLIGOSACCHARIDES 审中-公开

    公开(公告)号：WO2019115770A1

    公开(公告)日：2019-06-20

    申请号：PCT/EP2018/084966

    申请日：2018-12-14

    Applicant: UPFRONT CHROMATOGRAPHY A/S

    Inventor： MEINJOHANNS, Ernst ,  HARLOW, Kenneth

    IPC: B01D15/18 ,  A23C9/14 ,  B01D15/38 ,  A23C7/04

    Abstract: The present invention relates to a method for separating one or more oligosaccharide compound(s) from an oligosaccharide containing mixture, the method comprises the steps of (i) providing the oligosaccharide containing mixture; (ii) contacting the oligosaccharide containing mixture with a chromatographic support allowing one or more oligosaccharide compound(s) present in the oligosaccharide containing mixture to be retained by the chromatographic support; (iii) obtaining a unretained, flow through fraction from the chromatographic support comprising a protein, a cell, a cell debris, a nucleic acid and/or an enzyme; (iv) optionally washing the chromatographic support; (v) subjecting the chromatographic support to at least one elution buffer obtaining one or more oligosaccharide compound(s) from the chromatographic support; and wherein the chromatographic support comprises an adsorbent comprising one or more ligand capable of binding the one or more oligosaccharide compound(s) from the oligosaccharide containing mixture and wherein the one or more ligand comprise an a boronic acid compound, a serotonin compound, or a derivative thereof.

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