公开(公告)号：KR1020140013389A

公开(公告)日：2014-02-05

申请号：KR1020120080203

申请日：2012-07-23

Applicant: 아주대학교산학협력단

Inventor： 심우영 ,  이광 ,  백만정 ,  김재호 ,  현상미 ,  최기령 ,  박성수 ,  강진석 ,  심점순 ,  뉴엔덕도안

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6837 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/5014

Abstract: The present invention relates to a biomarker composition for diagnosing toxicity of nanoparticles, wherein the biomarker composition comprises at least one gene selected from aldehyde dehydrogenase, glutamic-pyruvate transaminase, glutamate dehydrogenase, glutamic oxaloacetic transaminase, glutamic acid decarboxylase, and glutamate-ammonia ligase, and causes a change in expression due to the exposure to nanoparticles. A biomarker according to the present invention is a genetic marker having a high correlation with the toxicity of nanoparticles. The biomarker of the present invention is used to confirm the toxicity of nanoparticles at a degree of more accurate and excellent detection. Therefore, the biomarker of the present invention is useful for monitoring or evaluating the toxicity of nanoparticles and useful as a tool for establishing effects on various diseases or the health, which is caused by the exposure to the nanoparticles.

Abstract translation: 本发明涉及一种用于诊断纳米颗粒的毒性的生物标志物组合物，其中该生物标志物组合物包含至少一种选自醛脱氢酶，谷氨酸 - 丙酮酸转氨酶，谷氨酸脱氢酶，谷氨酰氧乙酸转氨酶，谷氨酸脱羧酶和谷氨酸 - 氨连接酶的基因， 并且由于暴露于纳米颗粒而导致表达的改变。 根据本发明的生物标志物是与纳米颗粒的毒性高度相关的遗传标记。 本发明的生物标志物用于在更精确和优异的检测程度上确认纳米颗粒的毒性。 因此，本发明的生物标志物可用于监测或评估纳米颗粒的毒性，并且可用作建立对暴露于纳米颗粒引起的各种疾病或健康的作用的工具。

公开(公告)号：KR1020140013390A

公开(公告)日：2014-02-05

申请号：KR1020120080204

申请日：2012-07-23

Applicant: 아주대학교산학협력단

Inventor： 심우영 ,  이광 ,  백만정 ,  김재호 ,  현상미 ,  최기령 ,  박성수 ,  강진석 ,  심점순 ,  뉴엔덕도안

IPC: G01N33/00 ,  C12Q1/02 ,  G01N33/48

Abstract: The present invention relates to a method for evaluating toxicity of nanoparticles, and more particularly, to a method for evaluating toxicity of nanoparticles comprising the steps of: obtaining a sample of tissues or cells of a mammal exposed to nanoparticles; irradiating at least one selected from a group consisting of a glutamic acid content, a pyruvate content, whether mitochondria within a cell is damages or not, a reactive oxygen species (ROS) content and an ATP content within the sample; comparing with a control group. The method for evaluating toxicity of nanoparticles according to the present invention analyzes factors having high a correlation with the toxicity of nanoparticles. By using this, it is possible to more precisely detect the toxicity of nanoparticles with excellent detection sensitivity, and through this, it can be effectively used in monitoring or evaluating harmfulness of the toxicity of nanoparticles, as well as effectively used as a tool for examining various diseases, the effects on health or the like when exposed to nanoparticles.

Abstract translation: 本发明涉及一种用于评价纳米颗粒的毒性的方法，更具体地，涉及一种用于评估纳米颗粒毒性的方法，包括以下步骤：获得暴露于纳米颗粒的哺乳动物的组织或细胞样品; 照射来自由谷氨酸含量，丙酮酸含量组成的组中的至少一种，细胞内的线粒体是否损伤，样品中的活性氧（ROS）含量和ATP含量; 与对照组比较。 根据本发明的纳米颗粒的毒性评估方法分析与纳米颗粒的毒性高度相关的因素。 通过使用这种方法，可以更精确地检测具有优异检测灵敏度的纳米颗粒的毒性，通过这种方法，可以有效地用于监测或评估纳米颗粒毒性的有害性，并有效地用作检查的工具 各种疾病，当暴露于纳米颗粒时对健康等的影响。