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公开(公告)号：WO2017222180A1
公开(公告)日：2017-12-28
申请号：PCT/KR2017/004948
申请日：2017-05-12
Applicant: 아주대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/025 , C12Q1/6837 , C12Q1/6881 , C12Q1/6883 , C12Q2600/106 , C12Q2600/112 , C12Q2600/142 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
Abstract: 본 발명은 나노입자의 신경독성 여부 진단용 바이오마커 조성물에 관한 것으로서, MNPs@SiO 2 (RITC)와 같은 나노입자는 세포내 폴리아민 대사체, 예를들어 푸트레신은 증가시키고 스페르미딘과 스페르민은 감소시키며, 또한 폴리아민의 대사와 관련된 유전자인 ODC1, SAT1, PAOX 및 SRM1의 발현을 증가시켜 봉입체 형성을 증가시키고 신경 부전을 야기할 수 있는 바, 나노입자에 의한 세포내 응집체 형성을 통한 나노신경독성을 판단하는 지표로서 폴리아민 대사체 및 폴리아민 대사와 관련된 유전자를 이용하여 나노 신경독성 생지표를 제공할 수 있다.
Abstract translation: 本发明涉及用于诊断纳米颗粒和纳米颗粒如MNPs @ SiO 2（RITC）的神经毒性的生物标记组合物， 例如，傅托盘磨损和最小化的费米迪安和精胺减少，也可以增加ODC1的基因，SAT1，涉及到聚胺的代谢和PAOX SRM1的表达增加所形成的包涵体并引起神经功能障碍 棒，它可以通过使用与多胺代谢物和多胺代谢作为判断通过由纳米颗粒形成的细胞内聚集体的纳米神经毒性，以提供纳米神经毒性寿命指示器的索引相关联的基因。
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公开(公告)号：WO2021029673A1
公开(公告)日：2021-02-18
申请号：PCT/KR2020/010687
申请日：2020-08-12
Applicant: 아주대학교산학협력단
Abstract: 본 발명은 나노입자 및 환경에서 발생되는 미세입자에 대한 독성을 저해하는 조성물에 관한 것으로, 나노입자 또는 환경 유래 미세 입자로 유발된 세포 내 ATP 감소, 세포 생존율 감소, 세포의 염증 유발성 형태 변화 및 세포의 활성화가 본 발명에 따른 조성물에 의해 저해되는 것이 확인됨에 따라, 상기 조성물은 나노입자 및 미세입자의 독성에 대한 저감 물질로 유용하게 사용될 수 있는 가능성이 있다.
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公开(公告)号：KR101885135B1
公开(公告)日：2018-08-06
申请号：KR1020160076570
申请日：2016-06-20
Applicant: 아주대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/68
Abstract: 본발명은나노입자의신경독성여부진단용바이오마커조성물에관한것으로서, MNPs@SiO(RITC)와같은나노입자는세포내폴리아민대사체, 예를들어푸트레신은증가시키고스페르미딘과스페르민은감소시키며, 또한폴리아민의대사와관련된유전자인 ODC1, SAT1, PAOX 및 SRM1의발현을증가시켜봉입체형성을증가시키고신경부전을야기할수 있는바, 나노입자에의한세포내응집체형성을통한나노신경독성을판단하는지표로서폴리아민대사체및 폴리아민대사와관련된유전자를이용하여나노신경독성생지표를제공할수 있다.
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公开(公告)号：KR1020170062191A
公开(公告)日：2017-06-07
申请号：KR1020150167693
申请日：2015-11-27
Applicant: 아주대학교산학협력단 , 경상대학교산학협력단
Abstract: 본발명은, 기판, 상기기판의상면에안착배치되고일측에는응력인가부가형성된본체, 상기응력인가부에연결되도록상기본체에형성되며, 상기응력인가부의마주보는양측으로세포를포함하는균일한양의점탄성유체를공급되게가이드하는유입채널, 상기유입채널에직교형상으로배치되도록상기응력인가부의마주보는양측으로연결되게상기본체에형성되며, 상기응력인가부로유입된점탄성유체및 상기세포를배출하는배출채널을포함하는세포손상분석용미세유체소자를제공하여, 유입채널내측으로유입되는세포가혼합된점탄성유체를본체의응력인가부의마주보는양측에균일한양으로공급되게하고, 응력인가부로공급된점탄성유체는다시배출채널을통해유입채널에직교되는방향으로균일하게배출이이루어지게된다. 이때, 유입채널을통해응력인가부로유입되는점탄성유체의유동흐름에의해발생되는신장응력이가해진세포의손상되는조건을확인할수 있게하는바, 바이오리액터를이용한세포의안정적인배양을위한조건을결정할수 있게한다.
Abstract translation: 本发明包括衬底，形成于所述主体，以便定位在所述基板的上表面上就位，并且具有应力形成施加部主体，连接到所述应力施加一侧，均匀汉阳包括将单元电池具有相对的两侧的应力 被放置在正交形状的入口通道，用于供给使引导到被连接到相对的两侧的粘弹性流体的入口通道的应力部分设置在主体形成，施加用于排出粘弹性流体和细胞入口部放电的应力 对于细胞损伤分析提供的微流体装置包括信道到，粘弹性流体的细胞混合以被引入到内入口通道到相对的两侧应力施加在主体的部分和将被供应到均匀汉阳，粘弹性供给应力施加部 流体再次通过出口通道在垂直于入口通道的方向上均匀地排出。 此时，可以判断条件为巴的稳定培养物，使用生物反应器，其使您能够确定的拉伸应力施加由通过入口通道施加的粘弹性流体流动部的应力的流动流引起的细胞损伤的条件的小区 我们来做吧。
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公开(公告)号：KR1020140125662A
公开(公告)日：2014-10-29
申请号：KR1020130043771
申请日：2013-04-19
Applicant: 아주대학교산학협력단
Abstract: A cell culture substrate of the present invention has a micro-structure satisfying several perspectives that should be significantly considered to provide an effective three-dimensional cell culture environment. The micro-structure enables a cell to be cultured and aggregated in a flow-like three-dimensional micro pellet form, promotes redifferentiation of the cell while suppressing dedifferentiation of the cell during cell culture, suppresses development of cytoskeleton, and increases fluidity and mobility of the cell.
Abstract translation: 本发明的细胞培养基质具有满足几个观点的微观结构，应该被认为是提供有效的三维细胞培养环境。 微结构使细胞以流动状的三维微粒形式培养和聚集，促进细胞的再分化，同时抑制细胞培养过程中细胞的去分化，抑制细胞骨架的发育，并增加细胞骨架的流动性和流动性 细胞。
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Patent Agency Ranking6.发明公开
公开(公告)号：KR1020140024025A
公开(公告)日：2014-02-27
申请号：KR1020140009076
申请日：2014-01-24
Applicant: 아주대학교산학협력단
Abstract: The present invention relates to a method for manufacturing patterned substrates which patterns a substrate with a carbon nanotube Langmuir-Blodgett (LB) thin film using a micro contact printing method or a lift-off method, and to growth and differentiation control of stem cells using the same. By using the present invention: manufacturing can be simply completed by attaching and detaching a mold; patterns can be formed on curved surfaces so that patterns can be formed on various shaped substrates; and various shaped patterns can be formed so that the method for manufacturing patterned substrates can be applied in various industries. In addition, the present invention controls growth and differentiation of the stem cells through patterning of the carbon nanotube LB thin film so that the stem cells can be grew in short time and differentiation can be enhanced. [Reference numerals] (AA) Barrier; (BB) Carbon nanotube; (CC) Water; (DD) Transfer carbon nanotube LB thin film to PDMS mold; (EE) PDMS mold; (FF) Carbon nanotube; (GG) Heat at 80°C for 5 minutes after attaching on a substrate; (HH) Substrate; (II) Remove mold; (JJ) Carbon nanotube LB thinfilm pattern; (KK) Transfer the carbon nanotube LB thin film to the substrate; (LL) Carbon nanotube LB thin film; (MM) Heat at 80°C for 5 minutes after attaching the PDMS mold to the thin film; (NN) Remove mold
Abstract translation: 本发明涉及使用微接触印刷法或剥离法利用碳纳米管Langmuir-Blodgett（LB）薄膜对基板进行图案化的图案化基板的制造方法，以及使用 一样。 通过使用本发明：可以通过附接和分离模具简单地完成制造; 可以在弯曲表面上形成图案，使得可以在各种成形基板上形成图案; 并且可以形成各种形状的图案，使得用于制造图案化基板的方法可以应用于各种行业。 此外，本发明通过图案化碳纳米管LB薄膜来控制干细胞的生长和分化，使得干细胞可以在短时间内生长并且可以增强分化。 （附图标记）（AA）屏障; （BB）碳纳米管; （CC）水; （DD）将碳纳米管LB薄膜转移至PDMS模具; （EE）PDMS模具; （FF）碳纳米管; （GG）附着在基板上80℃加热5分钟; （HH）基板; （二）去除模具; （JJ）碳纳米管LB薄膜图案; （KK）将碳纳米管LB薄膜转移到基板上; （LL）碳纳米管LB薄膜; （MM）将PDMS模具附着在薄膜上之后，在80℃下加热5分钟; （NN）去除模具
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公开(公告)号：KR1020140013389A
公开(公告)日：2014-02-05
申请号：KR1020120080203
申请日：2012-07-23
Applicant: 아주대학교산학협력단
CPC classification number: C12Q1/6883 , C12Q1/6837 , C12Q2600/142 , C12Q2600/158 , G01N33/5014
Abstract: The present invention relates to a biomarker composition for diagnosing toxicity of nanoparticles, wherein the biomarker composition comprises at least one gene selected from aldehyde dehydrogenase, glutamic-pyruvate transaminase, glutamate dehydrogenase, glutamic oxaloacetic transaminase, glutamic acid decarboxylase, and glutamate-ammonia ligase, and causes a change in expression due to the exposure to nanoparticles. A biomarker according to the present invention is a genetic marker having a high correlation with the toxicity of nanoparticles. The biomarker of the present invention is used to confirm the toxicity of nanoparticles at a degree of more accurate and excellent detection. Therefore, the biomarker of the present invention is useful for monitoring or evaluating the toxicity of nanoparticles and useful as a tool for establishing effects on various diseases or the health, which is caused by the exposure to the nanoparticles.
Abstract translation: 本发明涉及一种用于诊断纳米颗粒的毒性的生物标志物组合物，其中该生物标志物组合物包含至少一种选自醛脱氢酶，谷氨酸 - 丙酮酸转氨酶，谷氨酸脱氢酶，谷氨酰氧乙酸转氨酶，谷氨酸脱羧酶和谷氨酸 - 氨连接酶的基因， 并且由于暴露于纳米颗粒而导致表达的改变。 根据本发明的生物标志物是与纳米颗粒的毒性高度相关的遗传标记。 本发明的生物标志物用于在更精确和优异的检测程度上确认纳米颗粒的毒性。 因此，本发明的生物标志物可用于监测或评估纳米颗粒的毒性，并且可用作建立对暴露于纳米颗粒引起的各种疾病或健康的作用的工具。
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公开(公告)号：KR101145057B1
公开(公告)日：2012-05-11
申请号：KR1020100039780
申请日：2010-04-29
Applicant: 아주대학교산학협력단
IPC: G01N30/72 , G01N33/487
Abstract: 본 발명은 기체크로마토그래피-질량분석기를 이용하여, 생체시료로부터 멜라토닌 및 이의 전구체를 동시에 분석하는 방법을 제공하는 것으로, 구체적으로 멜라토닌 및 이의 전구체를 두 번 유도체화 하고 이 때의 반응조건을 조절하여, 생체내에 미량으로 존재하고 있어서 분석이 어려운 멜라토닌과 산화되고 분해되기 쉬운 멜라토닌의 전구체들을 동시에 분석할 수 있는 방법을 제공한다.
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公开(公告)号：KR1020110120390A
公开(公告)日：2011-11-04
申请号：KR1020100039780
申请日：2010-04-29
Applicant: 아주대학교산학협력단
IPC: G01N30/72 , G01N33/487
Abstract: PURPOSE: A method for simultaneously analyzing melatonin and the precursor of the same based on a gas chromatography-mass spectrometry is provided to derivatize the precursor of the melatonin, which is easily oxidized or decomposed, into stable materials. CONSTITUTION: A method for simultaneously analyzing melatonin and the precursor of the same based on a gas chromatography-mass spectrometry includes the following: A mixture containing a bio specimen is reacted with ethyl chlorofomate at pH 6-8. The resultant is reacted with ethyl chlorofomate at pH 11-13. The resultant is extracted using a solvent containing diethylether and ethyl acetate in order to obtain an extract. The extract is reacted with pentafluoropropionic anhydride. The resultant is analyzed.
Abstract translation: 目的：提供一种基于气相色谱 - 质谱法同时分析褪黑素及其前体的方法，将褪黑激素的前体易于氧化或分解成稳定的物质。 构成：基于气相色谱 - 质谱法同时分析褪黑激素及其前体的方法包括以下步骤：将含有生物样品的混合物与pH 6-8的氯化肟酸乙酯反应。 所得物与pH为11-13的氯代甲酸乙酯反应。 使用含有乙醚和乙酸乙酯的溶剂萃取所得物，得到提取物。 萃取液与五氟丙酸酐反应。 分析结果。
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