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公开(公告)号：KR101808391B1
公开(公告)日：2017-12-12
申请号：KR1020150190588
申请日：2015-12-31
Applicant: 강원대학교산학협력단
IPC: C12Q1/32 , C12N9/04 , C12N9/02 , G01N21/64 , C07D265/38
Abstract: 본발명은 G6PD의활성도측정용조성물, 이를포함하는키트및 G6PD의활성도측정방법에관한것이다.
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公开(公告)号：KR101704914B1
公开(公告)日：2017-02-09
申请号：KR1020150115634
申请日：2015-08-17
Applicant: 강원대학교산학협력단
IPC: A61K31/56 , A61K36/258 , A61K36/68 , A61K9/00
Abstract: 본발명은당뇨성혈관누출(vascular leakage)에의한질병의예방또는치료용약제학적조성물에관한것으로, 본발명에따르면당뇨성망막병증을포함하는당뇨성혈관누출에의한질병의예방또는치료가가능하며, 다른여러당뇨성혈관합병증예방또는치료에도활용할수 있는효과가있다.
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公开(公告)号：KR101338401B1
公开(公告)日：2013-12-10
申请号：KR1020110128102
申请日：2011-12-02
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 피브리노겐 어레이를 사용하여 하나의 샘플에서 혈액응고인자 13(FXIII) 및 트랜스글루타미나제 2 (TG2)의 트랜스아미네이션 활성을 동시에 측정할 수 있는 온-칩 활성도 측정 시스템 및 상기 측정 시스템에 효과적으로 사용될 수 있는 혈액응고인자 13- 및 트랜스글루타미나제 2- 특이적인 활성도 측정에 최적화된 반응 조성물에 관한 것이다.
본 발명자는 버퍼, pH, 디티오트레이톨 및 5-(바이오틴아미도)펜틸아민의 농도와 같은 측정 조건을 최적화하였으며, 시료내 특이적 FXIII 및 TG2 활성들을 결정하는 식을 생성하였다. 본 발명자는 성공적으로 이 측정 시스템을 적용하여 포볼 12-미리스테이트 13-아세테이트 및 인터루킨-4로 분화된 THP-1 단핵세포에서 FXIII 및 TG2 활성의 변화를 모니터하였다. 본 활성 측정법은 FXIII 및 TG2 활성의 고속대량 결정을 위해 민감하고 적합하기 때문에, 세포 신호전달 및 심장혈관 병태생리학 연구에 있어 이들 이소자임들의 차별적 기능들을 조사하는데 강한 잠재력을 가지고 있다.-
公开(公告)号：KR1020100054378A
公开(公告)日：2010-05-25
申请号：KR1020080113283
申请日：2008-11-14
Applicant: 강원대학교산학협력단
IPC: C12Q1/52
CPC classification number: C12Q1/52 , C12Y203/02013 , G01N33/533
Abstract: PURPOSE: A method for measuring in situ activity of transglutaminase is provided to simultaneously analyze plural cells and tissues and to remarkably shorten analysis time. CONSTITUTION: A method for measuring situ activity of transglutaminase comprises: a step of preparing an array chip in which 3-aminopropyltrimethoxy silane is exposed; a step of treating with 5-(biotinamido)penthylamine to the live cells or tissues; a step of performing an amino exchange reaction to extract a cell extract containing a biotin-binding protein; a step of reacting the cell extract on a spot of an array chip; and a step of measuring in situ transglutaminase activity.
Abstract translation: 目的：提供一种测定转谷氨酰胺酶原位活性的方法，以同时分析多种细胞和组织，并显着缩短分析时间。 构成：测定转谷氨酰胺酶的原位活性的方法，其特征在于，制备其中露出3-氨基丙基三甲氧基硅烷的阵列芯片的步骤; 用5-（生物素酰氨基）戊胺处理活细胞或组织的步骤; 进行氨基交换反应以提取含有生物素结合蛋白的细胞提取物的步骤; 使细胞提取物在阵列芯片的点上反应的步骤; 以及测量原位转谷氨酰胺酶活性的步骤。
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公开(公告)号：KR1020170079719A
公开(公告)日：2017-07-10
申请号：KR1020150190588
申请日：2015-12-31
Applicant: 강원대학교산학협력단
IPC: C12Q1/32 , C12N9/04 , C12N9/02 , G01N21/64 , C07D265/38
Abstract: 본발명은 G6PD의활성도측정용조성물, 이를포함하는키트및 G6PD의활성도측정방법에관한것이다.
Abstract translation: 本发明涉及一种用于测量含G6PD组合物的活性，在此测得的G6PD活性的试剂盒和方法。
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Patent Agency Ranking
公开(公告)号：KR1020130061838A
公开(公告)日：2013-06-12
申请号：KR1020110128102
申请日：2011-12-02
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: A method for simultaneously measuring activities of blood coagulation factor 13 and transglutaminase 2 is provided to measure the activities of blood coagulation factor 13 and transglutaminase 2 in one sample. CONSTITUTION: A method for simultaneously measuring activities of blood coagulation factor 13 and transglutaminase 2 comprises: a step of preparing a standard array(array 1) for measuring activity of blood coagulation factor 13, a standard array(array 2) for measuring activity of transglutaminase 2, and an array(array 3) for simultaneously measuring blood coagulation factor 13 and transglutaminase 2 of a sample, in which fibrinogen is fixed on a plurality of spots; a step of performing transamidation between 5-(biotinamido)pentylamine(BAPA) and glutamine of fibrinogen; a step of introducing a fluorescence-labeled material to biotin and measuring fluorescence intensity; a step of making a standard curve of activity of blood coagulation factor 13 from the fluorescence intensity of array 1; a step of making a standard curve of activity of transglutaminase 2 from the fluorescence intensity of array 2; and a step of applying the standard curves to equation, and quantitatively measuring activity of blood coagulation factor 13 and transglutaminase 2.
Abstract translation: 目的：同时测定血液凝固因子13和转谷氨酰胺酶2活性的方法，用于测定一个样品中凝血因子13和转谷氨酰胺酶2的活性。 构成：同时测量凝血因子13和转谷氨酰胺酶2的活性的方法包括：制备用于测定凝血因子13的活性的标准阵列（阵列1）的步骤，用于测量转谷氨酰胺酶活性的标准阵列（阵列2） 2和用于同时测量样品的凝血因子13和转谷氨酰胺酶2的阵列（阵列3），其中纤维蛋白原固定在多个斑点上; 在5-（生物素酰氨基）戊胺（BAPA）和纤维蛋白原的谷氨酰胺之间进行酰胺化的步骤; 将荧光标记的材料引入生物素并测量荧光强度的步骤; 从阵列1的荧光强度制作凝血因子13活性的标准曲线的步骤; 从阵列2的荧光强度制作转谷氨酰胺酶2活性的标准曲线的步骤; 并将标准曲线应用于方程，并定量测定凝血因子13和转谷氨酰胺酶2的活性。
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公开(公告)号：KR1020100105254A
公开(公告)日：2010-09-29
申请号：KR1020090024188
申请日：2009-03-20
Applicant: 강원대학교산학협력단
IPC: G01N33/573 , G01N33/68 , G01N33/533
Abstract: PURPOSE: A protein chip for measuring MMP activation, in which a protein is fixed on a solid substrate and a method for measuring MMP activation using the same are provided. CONSTITUTION: A method for measuring MMP(matrix metalloproteinase) activation comprises: a step of preparing the protein chip; a step of contacting a sample containing MMP with the array chip; and a step of confirming substrate amount of decomposed MMP by SPR signal measurement to measure MMP activation. The MMP is selected from MMP-1 to MMP-23. The sample additionally contains Brij-35, Ca2^+ and Zn^2+. The MMP substrate is gelatin, proteoglycan, fibronectin, laminin, or elastin. The SPR signal measurement is performed using SPR biosensor or SPR(surface plasmon resonance imaging).
Abstract translation: 目的：提供一种用于测量MMP活化的蛋白质芯片，其中蛋白质固定在固体底物上，并提供了使用其测定MMP活化的方法。 构成：测量MMP（基质金属蛋白酶）活化的方法包括：制备蛋白质芯片的步骤; 将含有MMP的样品与阵列芯片接触的步骤; 以及通过SPR信号测定来确认分解的MMP的底物量以测量MMP活化的步骤。 MMP选自MMP-1至MMP-23。 样品另外含有Brij-35，Ca2 +和Zn2 +。 MMP底物是明胶，蛋白聚糖，纤连蛋白，层粘连蛋白或弹性蛋白。 使用SPR生物传感器或SPR（表面等离子体共振成像）进行SPR信号测量。
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公开(公告)号：KR1020080085517A
公开(公告)日：2008-09-24
申请号：KR1020070027178
申请日：2007-03-20
Applicant: 강원대학교산학협력단
Abstract: An analysis method of blood proteins is provided to reduce the analysis time by immediately analyzing the blood proteins through comparison with the pre-labeled standard proteins without labeling process, and rapidly read the analysis results, and simultaneously analyze multiple bloods with one standard sample. An analysis method of blood proteins comprises the steps of: immobilizing an antibody specifically binding to a protein(antibody) to be analyzed to the surface to prepare a protein chip; preparing a labeled standard protein identical to the protein to be analyzed; mixing the standard protein with a sample containing the protein to be analyzed, and spotting the mixture on the surface of the protein chip with antibody; and analyzing the intensity shown by the competitive immune response between the standard protein and the protein to be analyzed against the antibody of the protein chip with a fluorescence scanner. Further, the labeled standard protein is marked by biotin.
Abstract translation: 提供血液蛋白的分析方法，通过与未标记过程的预先标记的标准蛋白进行比较，立即分析血液蛋白，从而缩短分析时间，快速阅读分析结果，并同时用一个标准样品分析多个血液。 血液蛋白质的分析方法包括以下步骤：将要分析的蛋白质（抗体）特异性结合的抗体固定在表面以制备蛋白质芯片; 制备与待分析的蛋白质相同的标记的标准蛋白质; 将标准蛋白质与含有待分析蛋白质的样品混合，并用抗体将该混合物在蛋白质芯片表面上点样; 并用荧光扫描仪分析标准蛋白质与蛋白质芯片抗体之间的竞争性免疫反应强度。 此外，标记的标准蛋白质被生物素标记。
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公开(公告)号：KR100784463B1
公开(公告)日：2007-12-11
申请号：KR1020060136899
申请日：2006-12-28
Applicant: 강원대학교산학협력단
Abstract: A method for preparing a protein chip is provided to reduce the preparation time of the protein chip significantly, be easy to analyze a protein at low concentration by increasing the binding affinity between proteins through modification of a medium and analyze a plurality of proteins simultaneously by maintaining the hydrophobicity of a free portion of the protein chip. A method for preparing a protein chip comprises the steps of: (a) putting a metal chip obtained by depositing the surface of a glass substrate with gold in a reaction solution including a thiol compound having a terminal acid group to fix the thiol compound on the surface of the metal chip; (b) introducing the diamine compound into the acid group terminal of the thiol compound by reacting the thiol compound-fixed metal chip with a diamine compound such as ethylenediamine, hydrazine, 1,3-diaminopropane and putrescine to prepare a raw material; (c) reacting carboxymethyl-dextran with N-hydroxysuccinimide in the presence of carbodiimide by the following reaction formula(1) to prepare N-hydroxysuccinimide-dextran as a protein reception medium; and (d) reacting the prepared raw material with the protein receptor medium to form an amide-linked N-hydroxysuccinimide-dextran(AL NHS-dextran) at an amine group terminal of the raw material. In the reaction formula(1), n is an integer of at least 1. A protein chip prepared by the method comprises: a raw material prepared by introducing a diamine compound to a terminal(-OH) of an acid group of a thiol compound having the acid group at the terminal fixed on the metal chip; and N-hydroxysuccinimide dextran which is an amide-linked protein reception medium.
Abstract translation: 提供了制备蛋白质芯片的方法，以显着减少蛋白质芯片的制备时间，易于通过增加培养基的蛋白质之间的结合亲和力而分析低浓度的蛋白质，并通过维持同时分析多种蛋白质 蛋白质芯片的游离部分的疏水性。 一种制备蛋白质芯片的方法，包括以下步骤：（a）将金属片放入玻璃基片表面，用金填充在含有末端酸基团的硫醇化合物的反应溶液中，将硫醇化合物固定在 金属片表面; （b）通过硫醇化合物固定金属芯片与二胺化合物如乙二胺，肼，1,3-二氨基丙烷和腐胺反应，将二胺化合物引入硫醇化合物的酸基末端以制备原料; （c）通过以下反应式（1）在碳二亚胺的存在下使羧甲基葡聚糖与N-羟基琥珀酰亚胺反应，制备N-羟基琥珀酰亚胺 - 葡聚糖作为蛋白质接收介质; 和（d）使所制备的原料与蛋白质受体培养基反应，以在原料的胺基末端形成酰胺键合的N-羟基琥珀酰亚胺 - 葡聚糖（AL NHS-葡聚糖）。 在反应式（1）中，n为至少为1的整数。通过该方法制备的蛋白质芯片包括：通过将二胺化合物引入硫醇化合物的酸基的末端（-OH）而制备的原料 端子上的酸基固定在金属芯片上; 和N-羟基琥珀酰亚胺葡聚糖，其是酰胺键的蛋白质接收介质。
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公开(公告)号：KR1020060123835A
公开(公告)日：2006-12-05
申请号：KR1020050045420
申请日：2005-05-30
Applicant: 강원대학교산학협력단
Abstract: A method for analysis of protein chip by using a line-scanning mode is provided to enhance reliability of analysis results compared to the spot scan method by repeatedly analyzing in one spot, and increase analysis speed compared to the total scan method by performing the analysis along the linear line of spot centers. The method for analysis of protein arrays by using the line-scanning mode comprises the steps of: installing the protein arrays having a plurality of protein spots on the x-y stage(8), and mounting the prism(10) on the x-y stage; subjecting the light from a light source(1) to p-polarization; transmitting the p-polarized light to the prism; irradiating the incident light from the prism to the protein arrays along the lines of protein spot centers on the protein arrays; moving the x-y stage in a constant distance, and collecting the light signal reflected from the protein arrays through at least one optical fiber(11) with a spectroscope(12) to obtain the surface resonance plasmon signal; and quantitatively analyzing the surface resonance plasmon signal.
Abstract translation: 提供了一种通过线扫描模式分析蛋白质芯片的方法，通过在一个点重复分析，提高分析结果的可靠性，并通过执行分析沿着总扫描方法提高分析速度 点中心的线性线。 通过使用线扫描模式分析蛋白质阵列的方法包括以下步骤：在x-y台（8）上安装具有多个蛋白质斑点的蛋白质阵列，并将棱镜（10）安装在x-y台上; 对来自光源（1）的光进行p偏振; 将p偏振光透射到棱镜; 沿着蛋白质阵列上的蛋白点心线照射来自棱镜的入射光到蛋白质阵列; 以恒定距离移动x-y平台，并通过具有分光镜（12）的至少一个光纤（11）收集从蛋白质阵列反射的光信号，以获得表面共振等离子体信号; 并定量分析表面共振等离子体信号。
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