난과식물 바이러스 검정용 프라이머 및 이를 이용한 바이러스 검출방법
    11.
    发明授权
    난과식물 바이러스 검정용 프라이머 및 이를 이용한 바이러스 검출방법 有权
    用于检测ORCHID感染病毒的筛选程序和使用其的病毒筛选方法

    公开(公告)号:KR101139802B1

    公开(公告)日:2012-06-27

    申请号:KR1020090106557

    申请日:2009-11-05

    Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.

    토마토 황화 잎 말림 바이러스에 대한 저항성 품종을 판별하는 방법
    13.
    发明公开
    토마토 황화 잎 말림 바이러스에 대한 저항성 품종을 판별하는 방법 有权
    用于评估对TYLCV耐药性的方法

    公开(公告)号:KR1020130104078A

    公开(公告)日:2013-09-25

    申请号:KR1020120025233

    申请日:2012-03-12

    CPC classification number: C12Q1/6895 C12N15/8205 C12N15/8283 C12Q2600/13

    Abstract: PURPOSE: A method for determining a variety with resistance to tomato yellow leaf curl virus (TYLCV) is provided to enhance convenience and precision in resistivity evaluation and to cheaply and repetitively evaluate resistance. CONSTITUTION: A method for determining a variety with resistance to TYLCV comprises the step of infecting plant leaves with TYLCV strains. The method also comprises the step of measuring TYLCV-Ct/Actin-Ct value. The method additionally comprises the step of determining a resistant variety when the TYLCV-Ct/Actin-Ct value is 0.7 or greater. TYLCV includes at least three among TYLCV-IS, TYLCV-NS, TYLCV-YS, TYLCV-GJ, and TYLCV-Cgh. [Reference numerals] (AA) Bacchus; (BB) Supper sunroad; (CC) Ten ten

    Abstract translation: 目的:提供一种测定番茄黄叶卷曲病毒(TYLCV)耐药性的方法,以提高电阻率评估的便利性和精确性,并且便宜且重复地评估抗性。 构成:用于确定对TYLCV具有抗性的品种的方法包括用TYLCV毒株感染植物叶的步骤。 该方法还包括测量TYLCV-Ct / Actin-Ct值的步骤。 该方法还包括当TYLCV-Ct / Actin-Ct值为0.7或更大时确定抗性品种的步骤。 TYLCV包括TYLCV-IS,TYLCV-NS,TYLCV-YS,TYLCV-GJ和TYLCV-Cgh中的至少三个。 (附图标记)(AA)酒神; (BB)晚餐太阳; (CC)十十

    난과식물 바이러스 검정용 프라이머 및 이를 이용한 바이러스 검출방법
    17.
    发明公开
    난과식물 바이러스 검정용 프라이머 및 이를 이용한 바이러스 검출방법 有权
    用于检测ORCHID感染病毒的筛选程序和使用其的病毒筛选方法

    公开(公告)号:KR1020110049513A

    公开(公告)日:2011-05-12

    申请号:KR1020090106557

    申请日:2009-11-05

    CPC classification number: C12Q1/6844 C12N15/11 C12Q1/70

    Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.

    Abstract translation: 目的:提供用于检测兰花病毒的引物和使用其的检测方法,以简单地检测ORSV,CyMV和OFV的感染。 构成:用于检测兰花病毒的引物组含有:序列号1的ORSV-420F引物; 序列号2的ORSV-420R引物; 序列号3的CyMV-620F引物; 序列号4的CyMV-620R引物,序列号5的OFV-873F引物; 或序列号6的OFV-873R引物。用于检测兰花病毒的方法包括:使用从样品分离的引物和RNA进行RT-PCR和PCR的步骤; 以及通过PCR产物的大小确定病毒检测的步骤。

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