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公开(公告)号：KR101139802B1
公开(公告)日：2012-06-27
申请号：KR1020090106557
申请日：2009-11-05
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
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公开(公告)号：KR1020140109050A
公开(公告)日：2014-09-15
申请号：KR1020130023217
申请日：2013-03-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6888 , C12Q1/701 , C12Q2561/113 , C12Q2600/16
Abstract: Provided is a single tube-multiplex PCR primer set for diagnosing Tomato yellow leaf curl virus (TYLCV) comprising a PCR primer set of sequence number 1 to 2 and a PCR primer set of sequence number 3 to 4 to enable accurate diagnosis of TYLCV using tomato juice without separating nucleic acid of tomato by developing one primer set specifically detecting TYLCV infecting tomatoes and one tomato β-tubulin gene primer set for determining whether or not PCR works normally.
Abstract translation: 提供了一种用于诊断番茄黄叶卷曲病毒（TYLCV）的单管多重PCR引物组，其包含序列号1至2的PCR引物组和序列号3至4的PCR引物组，以使得能够使用番茄准确诊断TYLCV 通过开发一种特异性检测TYLCV感染番茄的引物组和一种用于确定PCR是否正常工作的番茄β-微管蛋白基因引物组，不分离番茄核酸的果汁。
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Patent Agency Ranking
公开(公告)号：KR1020130104078A
公开(公告)日：2013-09-25
申请号：KR1020120025233
申请日：2012-03-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12N15/8205 , C12N15/8283 , C12Q2600/13
Abstract: PURPOSE: A method for determining a variety with resistance to tomato yellow leaf curl virus (TYLCV) is provided to enhance convenience and precision in resistivity evaluation and to cheaply and repetitively evaluate resistance. CONSTITUTION: A method for determining a variety with resistance to TYLCV comprises the step of infecting plant leaves with TYLCV strains. The method also comprises the step of measuring TYLCV-Ct/Actin-Ct value. The method additionally comprises the step of determining a resistant variety when the TYLCV-Ct/Actin-Ct value is 0.7 or greater. TYLCV includes at least three among TYLCV-IS, TYLCV-NS, TYLCV-YS, TYLCV-GJ, and TYLCV-Cgh. [Reference numerals] (AA) Bacchus; (BB) Supper sunroad; (CC) Ten ten
Abstract translation: 目的：提供一种测定番茄黄叶卷曲病毒（TYLCV）耐药性的方法，以提高电阻率评估的便利性和精确性，并且便宜且重复地评估抗性。 构成：用于确定对TYLCV具有抗性的品种的方法包括用TYLCV毒株感染植物叶的步骤。 该方法还包括测量TYLCV-Ct / Actin-Ct值的步骤。 该方法还包括当TYLCV-Ct / Actin-Ct值为0.7或更大时确定抗性品种的步骤。 TYLCV包括TYLCV-IS，TYLCV-NS，TYLCV-YS，TYLCV-GJ和TYLCV-Cgh中的至少三个。 （附图标记）（AA）酒神; （BB）晚餐太阳; （CC）十十
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公开(公告)号：KR1020200144909A
公开(公告)日：2020-12-30
申请号：KR1020190073135
申请日：2019-06-19
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70
Abstract: BBWV2(Broad bean wilt virus 2), CYNMV(Chinese yam necrotic mosaic virus), 및 JYMV(Japanese yam mosaic virus) 3종의마(Diascorea spp.) 바이러스를동시에검출하기위한프라이머세트가개시된다.
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16.发明授权멜론괴저반점바이러스(ＭＮＳＶ)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트 및 이를 포함하는 진단 키트 有权用于检测来自梅隆和水的梅隆NECROTIC SPOT病毒的检测装置及其包装盒
公开(公告)号：KR101248059B1
公开(公告)日：2013-03-27
申请号：KR1020100122770
申请日：2010-12-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트, 이를 포함하는 진단 키트 및 이를 이용한 진단방법에 관한 것이다. 보다 자세하게는 서열번호 1 및 2로 표시되는 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트를 통하여 멜론 분리주 및 수박 분리주를 명확하게 구분하여 진단할 수 있다.
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公开(公告)号：KR1020110049513A
公开(公告)日：2011-05-12
申请号：KR1020090106557
申请日：2009-11-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12N15/11 , C12Q1/70
Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
Abstract translation: 目的：提供用于检测兰花病毒的引物和使用其的检测方法，以简单地检测ORSV，CyMV和OFV的感染。 构成：用于检测兰花病毒的引物组含有：序列号1的ORSV-420F引物; 序列号2的ORSV-420R引物; 序列号3的CyMV-620F引物; 序列号4的CyMV-620R引物，序列号5的OFV-873F引物; 或序列号6的OFV-873R引物。用于检测兰花病毒的方法包括：使用从样品分离的引物和RNA进行RT-PCR和PCR的步骤; 以及通过PCR产物的大小确定病毒检测的步骤。
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