Abstract:
PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
Abstract:
Provided is a single tube-multiplex PCR primer set for diagnosing Tomato yellow leaf curl virus (TYLCV) comprising a PCR primer set of sequence number 1 to 2 and a PCR primer set of sequence number 3 to 4 to enable accurate diagnosis of TYLCV using tomato juice without separating nucleic acid of tomato by developing one primer set specifically detecting TYLCV infecting tomatoes and one tomato β-tubulin gene primer set for determining whether or not PCR works normally.
Abstract:
PURPOSE: A method for determining a variety with resistance to tomato yellow leaf curl virus (TYLCV) is provided to enhance convenience and precision in resistivity evaluation and to cheaply and repetitively evaluate resistance. CONSTITUTION: A method for determining a variety with resistance to TYLCV comprises the step of infecting plant leaves with TYLCV strains. The method also comprises the step of measuring TYLCV-Ct/Actin-Ct value. The method additionally comprises the step of determining a resistant variety when the TYLCV-Ct/Actin-Ct value is 0.7 or greater. TYLCV includes at least three among TYLCV-IS, TYLCV-NS, TYLCV-YS, TYLCV-GJ, and TYLCV-Cgh. [Reference numerals] (AA) Bacchus; (BB) Supper sunroad; (CC) Ten ten
Abstract:
본 발명은 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트, 이를 포함하는 진단 키트 및 이를 이용한 진단방법에 관한 것이다. 보다 자세하게는 서열번호 1 및 2로 표시되는 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트를 통하여 멜론 분리주 및 수박 분리주를 명확하게 구분하여 진단할 수 있다.
Abstract:
PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.