公开(公告)号：KR1020040087224A

公开(公告)日：2004-10-13

申请号：KR1020030021504

申请日：2003-04-04

Applicant: 대한민국(농촌진흥청장)

Inventor： 최지영 ,  김근영 ,  김종길 ,  최영철 ,  김남정 ,  제연호

IPC: C12Q1/68

Abstract: PURPOSE: Primers for diagnosing disease by Entomopathogenic microsporidia, and a diagnostic method using the same are provided, thereby diagnosing disease by Entomopathogenic microsporidia at an early stage, and reducing the time and costs for prevention and treatment of disease by Entomopathogenic microsporidia. CONSTITUTION: The PCR primer for diagnosing disease by Entomopathogenic microsporidia is selected from NS1 primer having the nucleotide sequence of SEQ ID NO:1, NS2 primer of SEQ ID NO:2, NS3 primer of SEQ ID NO:3 and NS4 primer of SEQ ID NO:4. The PCR primer set for diagnosing disease by Entomopathogenic microsporidia is selected from NS1 primer having the nucleotide sequence of SEQ ID NO:1, NS2 primer of SEQ ID NO:2, NS3 primer of SEQ ID NO:3 and NS4 primer of SEQ ID NO:4, wherein the primer set is NS1-NS2, NS1-NS4 or NS3-NS4. The diagnostic method of disease by Entomopathogenic microsporidia comprises PCR amplification of a sample of insects or insect excretion using the PCR primer.

Abstract translation: 目的：提供通过昆虫病原微孢子虫诊断疾病的引物和使用其的诊断方法，从而在早期阶段通过昆虫病原微孢子虫诊断疾病，并通过昆虫病原微孢子虫减少预防和治疗疾病的时间和成本。 构成：通过昆虫病原微孢子虫诊断疾病的PCR引物选自具有SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的NS2引物，SEQ ID NO：3的NS3引物和SEQ ID NO：3的NS4引物的NS1引物 NO：4。 通过昆虫病原微孢子虫诊断疾病的PCR引物组选自具有SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的NS2引物，SEQ ID NO：3的NS3引物和SEQ ID NO：3的NS4引物的NS1引物 4，其中引物组为NS1-NS2，NS1-NS4或NS3-NS4。 昆虫病原微孢子虫病的诊断方法包括使用PCR引物对昆虫样品或昆虫排泄物进行PCR扩增。

公开(公告)号：KR100331169B1

公开(公告)日：2002-04-06

申请号：KR1019990054812

申请日：1999-12-03

Applicant: 대한민국(농촌진흥청장)

Inventor： 김근영 ,  서동상 ,  황재삼 ,  이상몽 ,  강석우

IPC: C12N15/00

Abstract: 본발명은미세유리주사침을이용하여 DNA를누에알에직접주입하는방법에관한것이다. 상기의방법은미세유리관끝을사면으로예리하게갈아날카롭게다듬은미세유리주사침으로, 외래유전자를함유하는 DNA용액을누에알껍질을뚫고, 알내부로한번에직접주사하는것으로서, 외래유전자를생식세포에직접발현시키는뛰어난효과가있다.

公开(公告)号：KR1020010054147A

公开(公告)日：2001-07-02

申请号：KR1019990054812

申请日：1999-12-03

Applicant: 대한민국(농촌진흥청장)

Inventor： 김근영 ,  서동상 ,  황재삼 ,  이상몽 ,  강석우

IPC: C12N15/00

Abstract: PURPOSE: A microinjection method of DNA into silkworm eggs using micro glass needle is provided, of which processes are easy to perform and which has superior transformation rate. Therefore, a transformed silkworm is mass-produced. CONSTITUTION: The micro glass needle is produced by cutting and grinding the end of a capillary glass tube. Thereby, DNA solution containing a foreign gene is injected into a silkworm egg with maintaining a certain angle between the egg and the needle, at once, in order to directly express the foreign gene in a reproductive cell. Wherein, the angle between the egg and the needle is preferably 30-50 degree.

Abstract translation: 目的：提供使用微玻璃针将DNA引入蚕卵的显微注射方法，其工艺简单，转化率高。 因此，转化的蚕是大量生产的。 构成：微型玻璃针是通过切割和研磨毛细管玻璃管的末端来生产的。 由此，将含有外来基因的DNA溶液一次注入到具有一定角度的蚕卵中，以便在生殖细胞中直接表达外源基因。 其中，鸡蛋与针之间的角度优选为30-50度。

公开(公告)号：KR100267742B1

公开(公告)日：2000-11-01

申请号：KR1019980000444

申请日：1998-01-10

Applicant: 대한민국(농촌진흥청장)

Inventor： 진병래 ,  윤은영 ,  강석우 ,  윤형주 ,  김근영 ,  김호락 ,  강석권

IPC: C12N15/85

Abstract: PURPOSE: Provided are the fluorescent silkworms which light green fluorescence by illumination of ultraviolet rays. And a producing method thereof using a recombinant baculovirus containing the green fluorescent protein gene is also provided. Wherein, the green fluorescent protein gene can be transferred into the next generation of silkworms. CONSTITUTION: The fluorescent silkworms are produced by the steps of: producing the recombinant autographa californica nuclear polyhedrosis virus(AcNPV) containing the green fluorescent protein gene which is positioned under regulation of polyhedrosis protein gene promoter by inserting the 720 bp of green fluorescent protein gene into baculovirus; and infecting the recombinant AcNPV into silkworms and feeding the silkworms at 25 deg. C, in which the silkworms show the green fluorescence in breast 4 days after injection and show the green fluorescence in total body 7 days after injection.

Abstract translation: 目的：提供通过照射紫外线来发出绿色荧光的荧光蚕。 还提供了使用含有绿色荧光蛋白基因的重组杆状病毒的生产方法。 其中绿色荧光蛋白基因可以转移到下一代的蚕中。 构成：通过以下步骤生产荧光蚕：通过将720bp的绿色荧光蛋白基因插入到含有绿色荧光蛋白基因的重组自体苜蓿核型多角体病毒（AcNPV）中，该绿色荧光蛋白基因位于多形成蛋白基因启动子调节下 杆状病毒; 并将重组AcNPV感染到蚕中，并在25℃下喂养蚕。 C，其中家蚕在注射后4天显示乳腺中的绿色荧光，并在注射后7天显示总体中的绿色荧光。