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公开(公告)号：KR1020170053287A
公开(公告)日：2017-05-16
申请号：KR1020150155588
申请日：2015-11-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은다수의채집용기를자동으로노출시켜, 채집을수행할수 있는멀티타입미생물포자채집장치에관한것으로, 본발명은, 일측이개구된노출부가구비되는케이스; 상기케이스내에위치하며, 다수개의채집용기가각각거치되는거치부; 상기거치부일측에수평길이방향으로위치하는수평암; 상기수평암 상에이동가능하게수직길이방향으로위치하는수직암; 및상기수직암 상에이동가능하도록위치하는이송부;를포함하며, 상기다수개의채집용기는상기이송부에의해기설정된순서로이송되어상기노출부로노출되는멀티타입미생물포자채집장치를제공하며, 이에의하면, 여러번의채집이필요할경우에다수의채집용기를 1회의채집이종료될때마다, 일일이교환하는번거로움이없도록한 번에여러개의포자채집용기를설치하고정해진시각에정해진시간동안채집을수행할수 있어야간이나타 업무로인하여접근이어려운경우에도편리하게효율적인미생물의채집이가능하고또한, 서로다른미생물의채집도한번의설정된과정을통하여수행할수 있다는장점을갖는다.
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公开(公告)号：KR1020160058612A
公开(公告)日：2016-05-25
申请号：KR1020140160326
申请日：2014-11-17
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A01N65/20 , A01N25/02 , A01N65/00 , A01N2300/00 , C07H17/07 , Y10S424/10
Abstract: 본발명은장안녹두의조추출물또는이의분획물을유효성분으로포함하는살충용조성물및 이를이용하여해충을살충하는방법에관한것이다. 보다구체적으로, 장안녹두의조추출물또는이의분획물을유효성분으로포함하는발명의조성물은팥바구미및 톱다리개미허리노린재에우수한살충효과를가지고있으며, 식물성천연살충제로서친환경적인바, 완전변태류및 불완전변태류해충에대한살충용조성물로유용하게사용될수 있다.
Abstract translation: 本发明涉及包含绿豆粗提物或其一部分作为活性成分的杀虫组合物，以及通过使用该杀虫组合物来排斥有害昆虫的方法。 更具体地说，根据本发明的包含绿豆粗提取物或其一部分作为活性成分的杀虫剂组合物在排斥中华绒螯蟹和鱼腥草中具有优异的杀虫作用，作为植物性天然杀虫剂是环境友好的，以及 可有效地用作关于美甲酰胺和半双子叶有害昆虫的杀虫组合物。
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公开(公告)号：KR1020150047957A
公开(公告)日：2015-05-06
申请号：KR1020130128070
申请日：2013-10-25
Applicant: 대한민국(농촌진흥청장)
IPC: C07K16/08 , C07K14/005 , G01N33/569
Abstract: 본발명은벼검은줄오갈병의발병원인이되는 RBSDV(Rice black streaked dwarf virus)의외피단백질과결합할수 있는항체또는항혈청, 상기항체또는항혈청이결합할수 있는항원단백질, 상기항체또는항혈청을포함하는벼검은줄오갈병진단용조성물, 상기조성물을포함하는벼검은줄오갈병진단용키트및 상기조성물또는키트를이용하여벼검은줄오갈병의발병여부를진단하는방법에관한것이다. 본발명의항원단백질또는항체를이용하면, 벼검은줄오갈병의원인이되는 RBSDV의외피단백질을검출할수 있으므로, 벼검은줄오갈병의조기진단에널리활용될수 있을것이다.
Abstract translation: 本发明涉及可与水稻黑条纹矮病毒（RBSDV）的皮层蛋白结合的抗体或抗血清，其引起稻黑条纹矮秆，可与抗体或抗血清组合的抗原蛋白，用于诊断的组合物 含有抗体或抗血清的黑色黑条纹矮秆，用于诊断含有该组合物的米黑条纹矮秆的试剂盒，以及通过使用该组合物或试剂盒发现水稻黑条纹矮化病的诊断方法。 本发明的抗原蛋白质或抗体可以检测作为水稻黑条纹矮秆的原因的RBSDV的皮质蛋白，从而用于早期诊断水稻黑条纹矮秆。
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公开(公告)号：KR1020090061845A
公开(公告)日：2009-06-17
申请号：KR1020070128814
申请日：2007-12-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A transformation method of soy bean is provided to produce the transgenic plant regardless of bean species and germination period, simplify the transformation process and save the expense. A method for transforming a soybean comprises: a step of culturing an agrobacterium which an expression vector containing a foreign target gene and marker gene for transforming the soybean is introduced and preparing suspension; a step of inoculating the suspension into embryo axis cross section of the soybean germination seed and co-culturing; a step of inducing transformed shoot in the co-cultured embryo axis cross section and selecting; a step of extending the selected shoot; and a step of inducing and purifying a root from the extended shoot.
Abstract translation: 提供大豆的转化方法，无论豆类和萌发期如何，都能生产转基因植物，简化转化过程，节省费用。 一种大豆转化方法包括：引入含有外来靶基因的表达载体和转化大豆的标记基因的农杆菌的培养步骤，制备悬浮液; 将悬浮液接种到大豆萌发种子的胚轴横截面并共培养的步骤; 在共培养胚轴切面诱导转化芽的选择; 扩展所选拍摄的步骤; 以及从延伸芽诱导和净化根的步骤。
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公开(公告)号：KR100496017B1
公开(公告)日：2005-06-16
申请号：KR1020020075497
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본 발명은 오이모자이크바이러스, 오이녹반모자이크바이러스, 큐리녹반모자이크바이러스, 수박모자이크바이러스2 및 쥬키니황화모자이크바이러스를 동시진단하기 위한 프라이머 조합을 개시한다.
본 발명에 의하면 항혈청을 이용한 진단방법보다 1,000배 이상 검출한계가 높으며, 지금까지 알려진 박과작물 5종 바이러스의 모든 계통에 대한 동시검출이 가능하다.-
Patent Agency Ranking
公开(公告)号：KR1020040047336A
公开(公告)日：2004-06-05
申请号：KR1020020075497
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: A primer combination for simultaneously detecting five cucurbit-infecting viruses is provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, and simultaneously detecting all kinds of cucurbit-infecting viruses. CONSTITUTION: The primer combination for simultaneously detecting five cucurbit-infecting viruses including cucumber mosaic virus(CMV), cucumber green mottle mosaic virus(CGMMV), kyuri green mottle mosaic virus(CGMMV), watermelon mosaic virus 2(WMV2) and zucchini yellow mosaic virus(ZYMV) comprises a combination primers consisting of the nucleotide sequences set forth in SEQ ID NOs: 5 and 11, the nucleotide sequences set forth in SEQ ID NOs: 15 and 22, the nucleotide sequences set forth in SEQ ID NOs: 27 and 29, the nucleotide sequences set forth in SEQ ID NOs: 34 and 40, and the nucleotide sequences set forth in SEQ ID NOs: 49 and 51, complementary nucleotide sequences with the nucleotide sequence of the combination primers, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.
Abstract translation: 目的：提供同时检测五种葫芦科感染病毒的引物组合，从而提高检测限度和灵敏度，比使用抗血清高约1000倍，同时检测各种葫芦科感染病毒。 构成：用于同时检测黄瓜花叶病毒（CMV），黄瓜绿斑驳花叶病毒（CGMMV），kyuri绿色斑马花叶病毒（CGMMV），西瓜花叶病毒2（WMV2）和西葫芦黄色马赛克的五种葫芦科感染病毒的引物组合 病毒（ZYMV）包含由SEQ ID NO：5和11所示的核苷酸序列，SEQ ID NO：15和22所示的核苷酸序列，SEQ ID NO：27所示的核苷酸序列和SEQ ID NO： 29，SEQ ID NO：34和40所示的核苷酸序列，以及SEQ ID NO：49和51所示的核苷酸序列，与组合引物的核苷酸序列的互补核苷酸序列及其突变的核苷酸序列，其中 突变选自碱基的缺失，碱的加入，碱的取代或其组合。
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公开(公告)号：KR101771963B1
公开(公告)日：2017-08-28
申请号：KR1020150156765
申请日：2015-11-09
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은옥수수깨씨무늬병(southern corn leaf blight)에특이적인프라이머쌍 및옥수수둥근무늬병(northern corn leaf spot)에특이적인프라이머쌍을포함하는, 옥수수깨씨무늬병및 둥근무늬병의동시진단용조성물, 옥수수깨씨무늬병및 둥근무늬병의진단용프라이머쌍, 상기프라이머쌍을포함하는옥수수깨씨무늬병및 둥근무늬병동시진단용키트및 상기프라이머쌍을이용하여옥수수로부터분리된 DNA를주형으로멀티플렉스 PCR을수행하여판별하는단계를포함하는, 옥수수깨씨무늬병및 둥근무늬병의동시진단방법에관한것이다. 본발명의주요 2종옥수수깨씨무늬병및 옥수수둥근무늬병의동시진단키트를이용한진단방법을이용하면, 같은바이폴라리스() 속에속하여유사한형태적및 균학적특징으로인해병원균의동정이쉽지않은상기 2 종의옥수수진균병에대하여간섭현상없이동시진단이가능하므로시간및 노력을절감할수 있으며, 이로써상기 2종진균병의신속한조기진단이가능하게함으로써옥수수깨씨무늬병및 옥수수둥근무늬병의예방, 종합관리체계확립및 건전종자확보에기여할수 있다.
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公开(公告)号：KR101740568B1
公开(公告)日：2017-05-29
申请号：KR1020150155588
申请日：2015-11-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은다수의채집용기를자동으로노출시켜, 채집을수행할수 있는멀티타입미생물포자채집장치에관한것으로, 본발명은, 일측이개구된노출부가구비되는케이스; 상기케이스내에위치하며, 다수개의채집용기가각각거치되는거치부; 상기거치부일측에수평길이방향으로위치하는수평암; 상기수평암 상에이동가능하게수직길이방향으로위치하는수직암; 및상기수직암 상에이동가능하도록위치하는이송부;를포함하며, 상기다수개의채집용기는상기이송부에의해기설정된순서로이송되어상기노출부로노출되는멀티타입미생물포자채집장치를제공하며, 이에의하면, 여러번의채집이필요할경우에다수의채집용기를 1회의채집이종료될때마다, 일일이교환하는번거로움이없도록한 번에여러개의포자채집용기를설치하고정해진시각에정해진시간동안채집을수행할수 있어야간이나타 업무로인하여접근이어려운경우에도편리하게효율적인미생물의채집이가능하고또한, 서로다른미생물의채집도한번의설정된과정을통하여수행할수 있다는장점을갖는다.
Abstract translation: 多型微生物孢子捕集装置技术领域本发明涉及一种能够自动地收集并收集多个收集容器的多型微生物孢子捕集装置。 分别安装在壳体中的多个收集容器， 水平臂，沿水平方向设置在安装部分的一侧; 垂直臂，垂直方向可移动地设置在水平臂上; 和垂直传送其被定位成是对臂可移动的，包括所述多个收集容器在由sanggiyi发射预定的顺序被输送和提供的多类型微生物孢子曝光的收集装置外露部分，由此 据，可以在每个时间时的多次收集需要单个集合结束多个收集容器，以便不必更换一次安装多个孢子收集容器和一个固定的时间内执行的收集上的固定时间的不便 必须收集的可能，而且，收集到的微生物高效方便地访问不同的微生物，即使肝脏是困难的，因为演艺事业也有，你可以通过重新设置在执行过程中的优势。
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公开(公告)号：KR1020140082079A
公开(公告)日：2014-07-02
申请号：KR1020120151352
申请日：2012-12-21
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12Q2537/143 , C12Q2563/113
Abstract: The present invention relates to a composition including a primer pair specific to rice dwarf virus (RDV), a primer pair specific to rice black-streaked dwarf virus (RBSDV) and a primer pair specific to rice stripe virus (RSV) for diagnosing rice viral diseases; a primer pair for diagnosing a rice viral disease; a kit including the primer pair for diagnosing a rice viral disease; and a method for simultaneous diagnosis of RDV, RBSDV and RSV including a determination step by performing multiplex RT-PCR using RNA separated from rice or insect vectors as a template using the primer pair. Using the diagnosis method using the kit for simultaneous diagnosis of major three kinds of rice viral diseases of the present invention enables simultaneous diagnosis of a complex infection contagion plant such that time and efforts can be reduced, thereby allowing a rapid early diagnosis of rice viral diseases, and thus contributing to establishment of a general management system of rice viral diseases and maintenance of high quality seeds.
Abstract translation: 本发明涉及包含对水稻矮化病毒（RDV）特异性的引物对，水稻黑条纹矮病毒（RBSDV）特异性引物对和用于诊断水稻病毒的水稻条纹病毒（RSV）特异性引物对）的组合物 疾病; 用于诊断水稻病毒性疾病的引物对; 包括用于诊断水稻病毒病的引物对的试剂盒; 以及通过使用引物对使用从稻或昆虫载体分离的RNA作为模板进行多重RT-PCR的用于同时诊断RDV，RBSDV和RSV的方法，包括确定步骤。 利用本发明的三种水稻病毒病毒同时诊断试剂盒的诊断方法，能够同时诊断复杂的感染传染病植物，从而可以减少时间和精力，从而可以快速早期诊断水稻病毒性疾病 ，从而有助于建立水稻病毒性疾病综合管理制度和维护优质种子。
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公开(公告)号：KR1020140081512A
公开(公告)日：2014-07-01
申请号：KR1020120151353
申请日：2012-12-21
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12Q2537/143 , C12Q2563/113
Abstract: The present invention relates to a composition including a primer pair specific to bacterial grain rot, a primer pair specific to bacterial brown streak and a primer pair specific to bacterial leaf blight for diagnosing rice bacterial diseases; a primer pair for diagnosing rice bacterial diseases; a kit including the primer pair for diagnosing rice bacterial diseases; and a method for simultaneous diagnosis of bacterial grain rot, bacterial brown streak and bacterial leaf blight, including a determination step by performing multiple PCRs using DNA separated from rice as a template using the primer pairs. The diagnosis method using the kit for simultaneous diagnosis of major three rice bacterial diseases of the present invention enables simultaneous diagnosis of a complex infection contagion plant such that time and efforts can be reduced, rapid early diagnosis of rice bacterial diseases can be possible, and thus the method can contribute to establishment of a general management system of rice bacterial diseases and maintain high quality seeds.
Abstract translation: 本发明涉及一种组合物，其特征在于，包括细菌颗粒腐烂特异性的引物对，细菌褐色条纹特异的引物对和用于诊断水稻细菌病的细菌性叶枯病特异性引物对; 用于诊断水稻细菌性疾病的引物对; 包括用于诊断水稻细菌性疾病的引物对的试剂盒; 以及用于同时诊断细菌颗粒腐烂，细菌褐色条纹和细菌性叶枯病的方法，包括使用引物对使用从水稻分离的DNA作为模板进行多个PCR的测定步骤。 使用本发明的三大水稻细菌性疾病同时诊断试剂盒的诊断方法能够同时诊断复杂的感染传染病植物，从而可以减少时间和努力，可以快速早期诊断水稻细菌病，因此 该方法可有助于建立水稻细菌性疾病综合管理体系，保持优质种子。
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