公开(公告)号：KR1020040103987A

公开(公告)日：2004-12-10

申请号：KR1020030035330

申请日：2003-06-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 조강희 ,  송관정 ,  황정환 ,  신용억

IPC: C12N15/10

Abstract: PURPOSE: A development method of SCAR marker linked to a gene for columnar growth habit in apple is provided, which method allows for discrimination of the columnar growth habit in young plant of apple. CONSTITUTION: The development method of SCAR(sequence characterized amplified region) marker linked to a gene for columnar growth habit in apple comprises the steps of: cross-fertilization of a standard type apple of "Fuji" with a columnar growth type apple of "Tuscan" to obtain a descendant apple F1, and analyzing properties of the descendant apple F1; selecting a marker linked to a gene for columnar growth habit through RAPD(Random amplified polymorphic DNA) analysis using BSA(bovine serum albumin); and sequencing the cloned RAPD marker, wherein the RAPD marker linked to the gene for columnar growth habit is a nucleotide sequence WB82670 of SEQ ID NO:1; and the SCAR marker is a pair of SCAR primers having the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3.

Abstract translation: 目的：提供一种连接苹果柱状生长习性基因的SCAR标记的开发方法，该方法可以区分苹果幼苗中的柱状生长习性。 构成：与苹果柱状生长习性基因连锁的SCAR（序列特征扩增区）标记物的开发方法包括以下步骤：将“富士”标准型苹果与“托斯卡纳”的柱状生长型苹果相互交叉 “获得后代苹果F1，分析后代苹果F1的属性; 通过使用BSA（牛血清白蛋白）的RAPD（随机扩增多态性DNA）分析，选择与基因进行柱状生长习性连接的标记; 并对克隆的RAPD标记进行测序，其中连接到用于柱状生长习性的基因的RAPD标记是SEQ ID NO：1的核苷酸序列WB82670; SCAR标记是具有SEQ ID NO：2和SEQ ID NO：3的核苷酸序列的一对SCAR引物。