Abstract:
The present invention relates to a sequence characterized amplified region (SCAR) marker for identification of Persimmon cultivars and uses thereof, and more specifically to a SCAR marker for identification of persimmon cultivars, a kit for identification of persimmon cultivars comprising a SCAR primer set, and a method for determining the variety of Persimmon cultivars using the SCAR primer set.
Abstract:
본 발명은 포도의 품종 판별용 SCAR 마커 및 이의 용도에 관한 것이다. 본 발명의 분자 마커를 이용하는 경우, 포도의 품종을 명확하게 구별할 수 있으므로 품종 육성권자에 대한 보호를 강화할 수 있고, 포도 육묘 및 재배시 제기되는 품종 혼입 문제를 해결하는데 기여할 수 있으며, 포도 품종의 분자 유전학적 연구 및 주요 형태적 특성 분석에도 이용될 수 있다.
Abstract:
PURPOSE: A sequence characterized amplified region (SCAR) marker for determining the variety of grapes is provided to definitely distinguish the variety of grapes, to strengthen protection of plant variety right holders, and to be used in a research of molecular genetics and morphological characteristics of the variety. CONSTITUTION: A SCAR marker for determining the variety of grapes contains one or more selected from the group consisting of G509-243 (sequence number 35), GG05_281 (sequence number 36), GU19_283 (sequence number 37), G116_319 (sequence number 38), G507_334 (sequence number 39), G427_348 (sequence number 40), GU13_380 (sequence number 41), G427_382 (sequence number 42), G119_411 (sequence number 43), GV01_424 (sequence number 44), GW04_463 (sequence number 45), G169_515 (sequence number 46), G116_539 (sequence number 47), GV04_618 (sequence number 48), GV01_678 (sequence number 49), GG05_689 (sequence number 50), and GA07_775 (sequence number 51). The SCAR marker is acquired by random amplified polymorphic DNA (RAPD) analysis. A method for determining the variety of grapes comprises the steps of: isolating genome DNA from a grape; amplifying a target sequence by amplification using an oligonucleotide SCAR primer set and the genome DNA as a template; and detecting the amplified product.
Abstract:
본 고안은 지주홀더(24) 및 화분(30)의 상단부에 마련된 지주 지지대 다리 고정부(34)에 하단이 고정되고, 지주(10)가 관통하는 상기 지주홀더(24)에 상단이 고정되는 하나 이상의 지주 지지대 다리(22)를 포함하는 화분용 지주 지지대(20)로서, 상기 지주 지지대 다리(22)는 식물 종의 성장 높이에 따라 화분용 지주 지지대(20)의 지주 고정 높이를 조절할 수 있도록 길이를 신장시키거나 단축시키는 것이 가능한 것임을 특징으로 하는 화분용 지주 지지대에 관한 것이다.
Abstract:
PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.
Abstract:
PURPOSE: A primer for diagnosing apple mosaic virus(ApMV) infection using SYBR Green I is provided to accurately diagnose the infection and to enhance detection efficiency. CONSTITUTION: A primer set for diagnosing apple mosaic virus(ApMV) infection contains a primer of sequence number 1 and a primer of sequence number 2. A method for real-time detection method for ApMV comprises: a step of performing real-time PCR using sample DNA or partial DNA using a marker and primer set; and a step of analyzing curve of the real-time PCR products. The marker is SYBR green I.
Abstract translation:目的:提供用于诊断使用SYBR Green I的苹果花叶病毒(ApMV)感染的引物,以准确诊断感染并提高检测效率。 构成:用于诊断苹果花叶病毒(ApMV)感染的引物组包含序列号1的引物和序列号为2的引物。用于ApMV的实时检测方法的方法包括:使用 使用标记物和引物组对样本DNA或部分DNA进行检测; 以及分析实时PCR产物曲线的步骤。 标记是SYBR绿I。