公开(公告)号：KR100552618B1

公开(公告)日：2006-02-15

申请号：KR1020030064437

申请日：2003-09-17

Applicant: 대한민국(농촌진흥청장)

Inventor： 김정희 ,  송관정 ,  황정환 ,  신용억

IPC: A01H1/00

Abstract: 본 발명은 국내 사과 재배의 주 품종인 '후지'를, 아그로박테리움(Agrobacterium)을 이용하여 형질 전환하는 기술에 관한 것이다. 보다 상세하게는, 외래 유전자가 도입된 아그로박테리움을 이용해 착색이 증진된 사과 '후지' 품종의 우량계통을 양성하여, 종래 착색에 소요되었던 노동력과 비용의 절감 효과를 가져와 생산의 효율성을 높일 수 있는 형질 전환 기술에 대한 것이다.
사과, 후지, 착색조절, 형질전환, 재분화

公开(公告)号：KR1020040103987A

公开(公告)日：2004-12-10

申请号：KR1020030035330

申请日：2003-06-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 조강희 ,  송관정 ,  황정환 ,  신용억

IPC: C12N15/10

Abstract: PURPOSE: A development method of SCAR marker linked to a gene for columnar growth habit in apple is provided, which method allows for discrimination of the columnar growth habit in young plant of apple. CONSTITUTION: The development method of SCAR(sequence characterized amplified region) marker linked to a gene for columnar growth habit in apple comprises the steps of: cross-fertilization of a standard type apple of "Fuji" with a columnar growth type apple of "Tuscan" to obtain a descendant apple F1, and analyzing properties of the descendant apple F1; selecting a marker linked to a gene for columnar growth habit through RAPD(Random amplified polymorphic DNA) analysis using BSA(bovine serum albumin); and sequencing the cloned RAPD marker, wherein the RAPD marker linked to the gene for columnar growth habit is a nucleotide sequence WB82670 of SEQ ID NO:1; and the SCAR marker is a pair of SCAR primers having the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3.

Abstract translation: 目的：提供一种连接苹果柱状生长习性基因的SCAR标记的开发方法，该方法可以区分苹果幼苗中的柱状生长习性。 构成：与苹果柱状生长习性基因连锁的SCAR（序列特征扩增区）标记物的开发方法包括以下步骤：将“富士”标准型苹果与“托斯卡纳”的柱状生长型苹果相互交叉 “获得后代苹果F1，分析后代苹果F1的属性; 通过使用BSA（牛血清白蛋白）的RAPD（随机扩增多态性DNA）分析，选择与基因进行柱状生长习性连接的标记; 并对克隆的RAPD标记进行测序，其中连接到用于柱状生长习性的基因的RAPD标记是SEQ ID NO：1的核苷酸序列WB82670; SCAR标记是具有SEQ ID NO：2和SEQ ID NO：3的核苷酸序列的一对SCAR引物。

公开(公告)号：KR1020050028329A

公开(公告)日：2005-03-23

申请号：KR1020030064437

申请日：2003-09-17

Applicant: 대한민국(농촌진흥청장)

Inventor： 김정희 ,  송관정 ,  황정환 ,  신용억

IPC: A01H1/00

CPC classification number: C12N15/8205 ,  C12N5/0025 ,  C12N15/8209 ,  C12N15/825 ,  C12N2500/34 ,  C12N2501/30

Abstract: A method for genetic transformation of 'Fuji' apple using Agrobacterium tumefaciens is provided, thereby improving color development in apples, and reducing labor and costs required for color development of apples, so that production efficiency of apples can be improved. The method for genetic transformation of 'Fuji' apple using Agrobacterium tumefaciens comprises the steps of: (1) culturing apple shoots and rooting the cultured apple shoots in a rooting medium; (2) culturing Agrobacterium tumefaciens containing a foreign gene; (3) inoculating the cultured Agrobacterium tumefaciens to the young leaf cuttings of the apple shoots; (4) culturing the Agrobacterium tumefaciens inoculated young leaf cuttings of the apple shoots in a co-culturing medium; and (5) regenerating the cultured young leaf cuttings of the apple shoots in a regeneration selection medium, wherein the foreign gene is DFR or B-peru.

Abstract translation: 提供了使用根癌农杆菌对“富士”苹果进行遗传转化的方法，从而改善了苹果的发色，降低了苹果发色所需的劳动和成本，从而可以提高苹果的生产效率。 使用根癌农杆菌对“富士”苹果进行遗传转化的方法包括以下步骤：（1）在生根培养基中培养苹果芽，培养苹果芽; （2）培养含有外来基因的根癌土壤杆菌; （3）将培养的根癌土壤杆菌接种到苹果芽的幼叶片中; （4）在共培养培养基中培养根癌土壤杆菌接种苹果芽的幼叶片; 和（5）在再生选择培养基中再生培养的苹果芽幼嫩叶片，其中外源基因是DFR或B-Peru。