brassicicola 의 병원성 관련 유전자인 AbSCD1의 특이적이고 정량적인 검출을 위한 프라이머 및 프로브에 관한 것으로, 더욱 구체적으로는 상기 프라이머 및 프로브를 사용한 실시간 PCR 방법은 검은무늬병원균을 특이적 및 정량적으로 검출 가능하므로, 검은무늬병의 진단 및 병의 진전에 대한 객관적 정량화에 이용될 수 있다.
Abstract:
PURPOSE: A plant which is transformed with a disease and catastrophe resistant gene is provided to improve productivity and to supply safe agricultural products. CONSTITUTION: A method for improving disease and catastrophe resistance in a plant comprises a step of increasing intracellular level of a polypeptide with an amino acid sequence of sequence number 2. A method for overexpressing the polypeptide comprises a step of transforming a recombinant vector in a plant or by introducing a gene encoding the polypeptide into a plant cell. The gene encoding the polypeptide has a base sequence of sequence number 1. The plant includes rice plant, barley, corn, rye, oat, lawn grass, hog millet, sugar cane, ryegrass, or orchard grass. A method for producing a plant with disease and catastrophe resistance comprises a step of transforming the recombinant expression vector to the plant.
Abstract:
PURPOSE: A rice blast resistant OsABP57 genes is provided to develop rice blast resistant breed, and to improve crop productivity. CONSTITUTION: A rice blast resistant protein has amino acid of sequence number 2. A gene encoding the protein has a base of sequence number 1. A transformant with improved rice blast resistance is prepared by transforming with a recombinant expression vector containing the gene of sequence number 1. The rice blast resistance is controlled by regulating protein level in a cell. A method for over-expressing the protein comprises: a step of preparing the recombinant expression vector and transforming a plant with the recombinant expression vector or a step of transducing the gene encoding the protein into plant cells. The plant is Oryza sativa, barley, wheat, rye, oat, grass, hog millet, corn, onion, leek, garlic, Anemarrhenae Rhizoma, Polygonatum falcatum, Veratri Rhizoma, bamboo, Cymbidium goeringii, Cymbidium ssp., Dendrobium phalaenopsis, Neofinetia falcate, Aerides japonicum, Cattleya ssp., Oncidium ssp., Brassia ssp., or Cymbidium Kanran.
Abstract:
The present invention relates to a Rhizoctonia solani AROM (RsAROM) gene-specific primer and to a method for detecting sheath blight by using the same, which can be used as a molecular biological method for diagnosing sheath blight by using a primer that can specifically detect RsAROM gene existing in a sheath blight genome and a probe to objectively quantify the development of sheath blight.