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公开(公告)号：WO2011049334A9
公开(公告)日：2011-04-28
申请号：PCT/KR2010/007117
申请日：2010-10-18
Abstract: 본 발명은 벼 유래의 OsLRP ( Oryza sativa leucine rich repeat protein)유전자를 포함하는 재조합 벡터로 식물 세포를 형질전환시켜 식물병 저항성을 증진시키는 방법, 상기 재조합 벡터로 형질전환된 식물병에 대한 저항성이 증진된 식물체 및 이의 종자, 벼 유래의 OsLRP 유전자를 포함하는 식물병 저항성 증진용 조성물 및 상기 재조합 벡터로 식물 세포를 형질전환시켜 식물체의 생산성을 향상시키는 방법에 관한 것이다.
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公开(公告)号：KR101459532B1
公开(公告)日：2014-11-12
申请号：KR1020130006540
申请日：2013-01-21
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 무름병 저항성이 증가한 형질전환 식물체에 대한 것이다. 보다 상세하게는 BrWRKY12 유전자를 포함하는 형질전환용 벡터로 식물세포를 형질전환하여 BrWRKY12 유전자를 과발현하여 무름병에 대해 높은 저항성을 가진 형질전환 식물체에 대한 것이다.
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公开(公告)号：KR101424101B1
公开(公告)日：2014-07-28
申请号：KR1020120069757
申请日：2012-06-28
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 특이도 및 민감도가 우수한 프라이머 세트 및 프로브를 포함하는 벼 도열병균 검출용 조성물 및 이를 이용해 벼 도열병균을 정량적으로 검출하는 방법을 제공한다. 본 발명에 따른 상기 벼 도열병균 검출용 조성물은 벼 도열병의 분자생물학적 예찰 시스템을 정착시킬 수 있고 벼 도열병균 감염의 객관적인 수치화가 가능하도록 한다.
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公开(公告)号：KR1020130054545A
公开(公告)日：2013-05-27
申请号：KR1020110119974
申请日：2011-11-17
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8281 , C07K14/415 , C12N15/8205 , C12N15/8216 , C12N15/8251
Abstract: PURPOSE: A transgenic plant which is prepared by transforming with a bacterial wilt resistant gene is provided to grow a variety with bacterial wilt resistance and to remarkably improve productivity of plants, especially solanaceae plants. CONSTITUTION: A method for improving bacterial wilt resistance in a plant comprises a step of increasing intracellular level of a polypeptide with an amino acid sequence of sequence number 2. The method also comprises a step of overexpressing the polypeptide. A gene encoding the polypeptide has a base sequence of sequence number 1. A method for producing a plant with resistance to bacterial wilt comprises a step of transforming a recombinant expression vector containing a gene encoding the polypeptide into a plant.
Abstract translation: 目的：提供通过用抗病性基因转化制备的转基因植物，以生长具有抗病性的抗性品种，并显着提高植物，特别是茄科植物的生产力。 构成：用于提高植物中抗病性的方法包括用序列号2的氨基酸序列增加多肽的细胞内水平的步骤。该方法还包括过表达多肽的步骤。 编码多肽的基因具有序列号1的碱基序列。用于产生对细菌性枯萎病具有抗性的植物的方法包括将含有编码该多肽的基因的重组表达载体转化到植物中的步骤。
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公开(公告)号：KR101258698B1
公开(公告)日：2013-05-13
申请号：KR1020110001902
申请日：2011-01-07
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은무름병저항성을증진시키는방법에관한것으로서, RTP1(Resistance to1) 유전자를포함하는형질전환용벡터로식물세포를형질전환하여 RTP1 유전자를과발현시키는단계를포함하는것을특징으로한다. 본발명에따른형질전환체는무름병에대한높은저항성을가질수 있다.
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Patent Agency Ranking
公开(公告)号：KR101024112B1
公开(公告)日：2011-03-29
申请号：KR1020090001958
申请日：2009-01-09
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물병 저항성을 증진시키는 방법으로서, 더욱 상세하게는 서열번호 1의 염기서열을 갖는 OsHRL(Oryza sativa HR-like lesion-inducing gene) 유전자를 포함하는 형질전환용 벡터로 식물 세포를 형질전환하여 OsHRL 유전자를 과발현시키는 단계를 포함하는 식물병 저항성을 증진시키는 방법에 관한 것이다.
본 발명에 따르면, 병 저항성 작물개발을 위한 분자육종소재로 이용가능하며 타 작물에도 이용가능할 것으로 기대된다. 또한, 병 저항성 품종육성으로 농약사용절감과 생산성 향상 및 안전한 농산물 공급이 가능하다는 이점이 있다.-
公开(公告)号：KR1020100082587A
公开(公告)日：2010-07-19
申请号：KR1020090001958
申请日：2009-01-09
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for enhancing plant disease resistance is provided to use in cultivating other crops and to improve crop productivity. CONSTITUTION: A method for enhancing plant disease resistance comprises a step of transforming plant cells with a vector containing OsHRL(Oryza sativa HR-like lesion-inducing gene) gene of sequence number 1 and a step of overexpressing OsHRL gene. The plant disease is Bacterial blight.
Abstract translation: 目的：提高植物抗病性的方法，用于培育其他作物和提高作物生产力。 构成：增强植物抗病性的方法包括用含有序列1的OsHRL（水稻HR样病变诱导基因）基因和OsHRL基因过表达的载体转化植物细胞的步骤。 植物病是细菌病。
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8.发明公开안토시아닌 생합성 억제 활성을 가진 ＭＹＢ６０ 유전자와상기 유전자가 도입된 형질전환 상추 및 그 제조방법 有权用于抑制蒽醌生物合成的MYB60基因，MYB60基因被转移的转基因及其制备方法
公开(公告)号：KR1020070110615A
公开(公告)日：2007-11-20
申请号：KR1020060043300
申请日：2006-05-15
Applicant: 대한민국(농촌진흥청장)
Abstract: A MYB60 gene is provided to inhibit biosynthesis of anthocyanin, so that it is useful for change of flower color of flower plants and for development of new varieties of flower plants. A transgenic lettuce containing the same MYB60 gene is also provided to verify anthocyanin biosynthesis-inhibiting effects of the gene through phenotype by using a plant system. A MYB60 gene inhibiting biosynthesis of anthocyanin has the nucleotide sequence of SEQ ID NO:1. A vector 22103-MYB60 contains the MYB60 gene. A transformed Agrobacterium tumefaciens EHA105(KACC 95045P) is produced with the vector 22103-MYB60. A method for preparing a transgenic lettuce comprises the steps of: (a) preparing an expression vector containing the MYB60 gene and a basta resistant bar gene as a selection marker; (b) producing Agrobacterium tumefaciens EHA105(KACC 95045P) by transforming with the expression vector; (c) co-culturing cotyledon of a lettuce plant with the transformed Agrobacterium tumefaciens EHA105(KACC 95045P); (d) selecting shoots of lettuce plant from a selection medium and inducing plants from the shoots in plant-inducing medium; and (e) transplanting the induced lettuce plants to an in vitro medium and the soil to acclimate them.
Abstract translation: 提供了一种MYB60基因来抑制花青素的生物合成，因此可用于改变花卉植物的花色和开发新品种的花卉植物。 还提供了含有相同MYB60基因的转基因莴苣，以通过使用植物系统来验证基因通过表型的花青素生物合成抑制作用。 抑制花青素生物合成的MYB60基因具有SEQ ID NO：1的核苷酸序列。 载体22103-MYB60含有MYB60基因。 用载体22103-MYB60生产转化的根癌土壤杆菌EHA105（KACC 95045P）。 一种制备转基因莴苣的方法包括以下步骤：（a）制备含有MYB60基因和巴斯坦耐药基因作为选择标记的表达载体; （b）通过用表达载体转化产生根癌土壤杆菌EHA105（KACC 95045P）; （c）用转化的根癌土壤杆菌EHA105（KACC 95045P）共同培养莴苣植物的子叶; （d）从选择培养基中选择莴苣植物芽，并诱导植物诱导培养基中的芽; 和（e）将诱导的莴苣植物移植到体外培养基和土壤以适应它们。
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公开(公告)号：KR1020040050634A
公开(公告)日：2004-06-16
申请号：KR1020020078495
申请日：2002-12-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
CPC classification number: C12N15/8273
Abstract: PURPOSE: A gene related to dehydration tolerance of potato and dehydration-tolerant plant using the same are provided, thereby amplifying the expression of the gene when the plant is faced with dehydration, so that the plant can grow under dehydration condition. CONSTITUTION: The gene StRD22 related to dehydration tolerance of potato is isolated from potato and has the nucleotide sequence set forth in SEQ ID NO: 1, wherein the gene StRD22 is isolated by the steps of: freezing potato treated at 4 deg. C for 8 hours and untreated potato and pulverizing them; extracting RNA from the pulverized potatoes; isolating poly A+RNA from the extracted total RNA; subjecting the poly A+RNA to RNase H-reverse transcriptase to synthesize first strand cDNA; removing RNA using RNase and synthesizing second strand cDNA using DNA polymerase I; inserting the synthesized cDNA into Uni-zap XR vector; in vitro packaging the cDNA inserted vector; infecting E. coli with the cDNA inserted vector to determine Phage titer and amplify the genes; and sequencing the genes.
Abstract translation: 目的：提供一种与马铃薯和脱水耐受性相关的脱水耐性相关基因，从而在植物面临脱水时扩增基因的表达，使植物在脱水条件下生长。 构成：与马铃薯的脱水耐性相关的基因StRD22与马铃薯分离，具有SEQ ID NO：1所示的核苷酸序列，其中通过以下步骤分离基因StRD22：将4℃处理的马铃薯冷冻。 8小时，未处理的马铃薯并粉碎; 从粉碎的马铃薯中提取RNA; 从提取的总RNA中分离聚A + RNA; 将聚A + RNA转染至RNA酶H逆转录酶合成第一链cDNA; 使用RNA酶除去RNA并使用DNA聚合酶I合成第二链cDNA; 将合成的cDNA插入到Uni-zap XR载体中; 体外包装cDNA插入载体; 用cDNA插入载体感染大肠杆菌以确定噬菌体滴度并扩增基因; 并对基因进行测序。
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公开(公告)号：KR101642772B1
公开(公告)日：2016-07-27
申请号：KR1020140158533
申请日：2014-11-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은고추탄저병검출용조성물및 이를이용한고추탄저병의검출방법에관한것이다. 본발명에따른고추탄저병검출용조성물은고추탄저병유전체에존재하는세포자가포식유전자(AUTOPHAGY8 gene; CaATG8)를특이적으로검출하여고추탄저병의진전량을객관적으로수치화할수 있으므로, 고추탄저병의분자생물학적진단방법으로유용하게이용할수 있다.
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