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11.发明公开멜론괴저반점바이러스(ＭＮＳＶ)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트 및 이를 포함하는 진단 키트 有权用于检测来自梅隆和水的梅隆NECROTIC SPOT病毒的检测装置及其包装盒
公开(公告)号：KR1020120061449A
公开(公告)日：2012-06-13
申请号：KR1020100122770
申请日：2010-12-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/68 , C12Q2600/16
Abstract: PURPOSE: A primer set for diagnosing MNSV from melon and watermelon and a kit are provided to definitely diagnose MNSV developed in melon and watermealon. CONSTITUTION: A primer set for diagnosing MNSV from melon and watermelon is denoted by sequence numbers 1 and 2. A primer set for simultaneously diagnosing MNSV from melon and watermelon has a primer set of sequence numbers 1 and 2 and sequence number 3 and 4. The primer set has a primer set of sequence numbers 1 and 2 and sequence number 5 and 6. A kit for diagnosing MNSV from melon and watermelon contains the primer set.
Abstract translation: 目的：用于诊断来自西瓜和西瓜的MNSV的引物组和试剂盒，以确定诊断在瓜和威瓜中开发的MNSV。 构成：用于从瓜类和西瓜诊断MNSV的引物组由序列号1和2表示。用于同时诊断来自甜瓜和西瓜的MNSV的引物组具有序列号1和2以及序列号3和4的引物组。 引物组具有序列号1和2以及序列号5和6的引物组。用于诊断来自瓜和西瓜的MNSV的试剂盒含有引物组。
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公开(公告)号：KR1020110072681A
公开(公告)日：2011-06-29
申请号：KR1020090129716
申请日：2009-12-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q2521/101 , C12Q2521/107 , C12Q2527/101 , C12Q2537/143
Abstract: PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.
Abstract translation: 目的：提供诊断INSV和TSWV的双引物，专门诊断INSV和TSWV，提高检测限。 构成：用于诊断INSV（凤仙桃坏死斑点病毒）和TSWV（番茄斑点枯萎病毒）的双链体引物含有寡核苷酸引物组，其含有序列号为15以上的连续核苷酸片段的15个以上连续核苷酸片段的寡核苷酸，15个以上序列号的连续核苷酸片段 2.用于同时诊断INSV和TSWV的试剂盒含有用于逆转录和扩增的引物组和试剂。
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公开(公告)号：KR1020200144909A
公开(公告)日：2020-12-30
申请号：KR1020190073135
申请日：2019-06-19
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70
Abstract: BBWV2(Broad bean wilt virus 2), CYNMV(Chinese yam necrotic mosaic virus), 및 JYMV(Japanese yam mosaic virus) 3종의마(Diascorea spp.) 바이러스를동시에검출하기위한프라이머세트가개시된다.
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15.发明授权멜론괴저반점바이러스(ＭＮＳＶ)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트 및 이를 포함하는 진단 키트 有权用于检测来自梅隆和水的梅隆NECROTIC SPOT病毒的检测装置及其包装盒
公开(公告)号：KR101248059B1
公开(公告)日：2013-03-27
申请号：KR1020100122770
申请日：2010-12-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트, 이를 포함하는 진단 키트 및 이를 이용한 진단방법에 관한 것이다. 보다 자세하게는 서열번호 1 및 2로 표시되는 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트를 통하여 멜론 분리주 및 수박 분리주를 명확하게 구분하여 진단할 수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020110049513A
公开(公告)日：2011-05-12
申请号：KR1020090106557
申请日：2009-11-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12N15/11 , C12Q1/70
Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
Abstract translation: 目的：提供用于检测兰花病毒的引物和使用其的检测方法，以简单地检测ORSV，CyMV和OFV的感染。 构成：用于检测兰花病毒的引物组含有：序列号1的ORSV-420F引物; 序列号2的ORSV-420R引物; 序列号3的CyMV-620F引物; 序列号4的CyMV-620R引物，序列号5的OFV-873F引物; 或序列号6的OFV-873R引物。用于检测兰花病毒的方法包括：使用从样品分离的引物和RNA进行RT-PCR和PCR的步骤; 以及通过PCR产物的大小确定病毒检测的步骤。
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公开(公告)号：KR101491788B1
公开(公告)日：2015-02-13
申请号：KR1020130023217
申请日：2013-03-05
Applicant: 대한민국(농촌진흥청장)
Abstract: 토마토에 발생하는 TYLCV를 특이적으로 진단할 수 있는 프라이머 1종 세트와 PCR의 정상 작동 유무를 판단할 수 있는 토마토 베타튜불린 (β-tubulin) 유전자 프라이머 1종 세트를 개발하여 토마토의 핵산 분리 없이 토마토 즙액을 가지고 바로 TYLCV의 진단을 정확하게 가능하도록 하고자 서열목록 1 내지 2의 PCR 프라이머 세트 및 서열번호 3 내지 4의 PCR 프라이머 세트로 구성된 토마토황화잎말림 바이러스 진단을 위한 단일 튜브-멀티플렉스 PCR 프라이머 세트를 제공한다.
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公开(公告)号：KR101457946B1
公开(公告)日：2014-11-04
申请号：KR1020120025233
申请日：2012-03-12
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 TYLCV에 대한 저항성 품종을 판정하는 방법에 관한 것으로, 본 발명에 따른 판정 방법을 이용할 경우, 저항성 평가의 편리성 및 정밀성이 증대되고, 분리주의 보전이 용이하여 적은 비용으로 반복적으로 평가할 수 있다.
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19.发明公开
公开(公告)号：KR1020120115498A
公开(公告)日：2012-10-18
申请号：KR1020127015778
申请日：2011-07-14
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8283
Abstract: PURPOSE: A manufacturing method of transformed potato plant with multifariousness virus resistance and a transformed potato plant manufactured by using the same are provided to increase potato productivity and to be used as a virus resistance parent. CONSTITUTION: A transformed potato plant is obtained by being transformed into an expression vector pK7GWIWG 2 which includes a gene fusing more than 3 kinds of virus genes selected from potato mop-top virus gene of the sequence number 5, PVY(Potato virus Y) gene of the sequence number 1(SEQ ID NO:1), PLRV(Potato leafroll virus) gene of the sequence number 2,PVA(Potato virus A) gene of the sequence number 3, or TRV(Tobacco rattle virus) gene of the sequence number 4 in sense and anti-sense direction. A manufacturing method of the multifariousness virus resistant transformed potato plant comprises the following steps: transforming Agrobacterium tumefaciens into an expression vector pK7GWIWG 2(I)/ YLRATRPM including YLRATRPM gene of the sequence number 6; and transforming potato cells by cocltivating agrobacterium and potato cells.
Abstract translation: 目的：提供具有多重抗病毒性的转化马铃薯植物的制造方法和使用该方法制造的转化马铃薯植物，以增加马铃薯的生产力并用作抗病性亲本。 构成：通过转化成表达载体pK7GWIWG2获得转化的马铃薯植物，该表达载体包括融合3种以上的序列号为5的马铃薯蚊虫 - 顶端病毒基因（PVY（马铃薯病毒Y）基因）的病毒基因的基因 序列号2的序列号1（SEQ ID NO：1），PLRV（马铃薯卷叶病毒）基因，序列号3的PVA（马铃薯病毒A）基因或序列的TRV（烟草恙虫病毒）基因 在感觉和反感方向上的数字4。 一种多重病毒抗性转化马铃薯植物的制备方法包括以下步骤：将根癌农杆菌转化成包含序列号6的YLRATRPM基因的表达载体pK7GWIWG 2（I）/ YLRATRPM; 并通过共同诱导农杆菌和马铃薯细胞转化马铃薯细胞。
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公开(公告)号：KR101139802B1
公开(公告)日：2012-06-27
申请号：KR1020090106557
申请日：2009-11-05
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
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