公开(公告)号：KR1020080025791A

公开(公告)日：2008-03-24

申请号：KR1020060090389

申请日：2006-09-19

Applicant: 박근형 ,  재단법인 전남생물산업진흥원

Inventor： 박근형 ,  지수현 ,  조정기 ,  문제학 ,  정경희

IPC: C07H15/203

CPC classification number: C07H15/04 ,  C07H1/08

Abstract: A novel 3-hydroxy-4-methoxyphenyl-6-O-(4-hydroxylbenzoyl)-beta-D- glucopyranoside isolated from leaves of Camellia japonica is provided to show radical scavenging activity, thereby being used as an antioxidizing agent. A novel material having anti-oxidative activity is 3-hydroxy-4-methoxyphenyl-6-O-(4-hydroxylbenzoyl)-beta-D-glucopyranoside isolated by obtaining a hot-water extract of leaves of Camellia japonica, fractionating the extract into an aqueous solution fraction and an ethylacetate soluble neutral fraction using a buffer solution having a pH of 8.0(5% NaHCO3) and ethylacetate, purifying the ethylacetate soluble neutral fraction through a Sephadex LH-20 column chromatography to purify an active fraction and then isolating the desired product through HPLC using a methanol-water solvent system.

Abstract translation: 提供从山茶花叶分离的新型3-羟基-4-甲氧基苯基-6-O-（4-羟基苯甲酰基）-β-D-吡喃葡萄糖苷，以显示自由基清除活性，从而用作抗氧化剂。 具有抗氧化活性的新型材料是通过获得山茶花叶片的热水提取物分离的3-羟基-4-甲氧基苯基-6-O-（4-羟基苯甲酰基）-β-D-吡喃葡萄糖苷，将提取物分馏 水溶液馏分和乙酸乙酯可溶性中性馏分，使用pH为8.0（5％NaHCO 3）和乙酸乙酯的缓冲溶液，通过Sephadex LH-20柱色谱纯化乙酸乙酯可溶性中性馏分，纯化活性馏分，然后分离 使用甲醇 - 水溶剂系统通过HPLC进行所需产物。

公开(公告)号：KR1020050105910A

公开(公告)日：2005-11-08

申请号：KR1020040032030

申请日：2004-05-03

Applicant: 박근형

Inventor： 박근형 ,  조정용 ,  장미영 ,  문제학 ,  최기현

IPC: A61K31/7048 ,  A61K31/522 ,  A61K36/13

CPC classification number: A61K31/7048 ,  A61K31/522 ,  A61K36/72 ,  A61K2236/33 ,  A61K2236/51

Abstract: 헛개나무(
Hovenia dulcis Thunb) 줄기와 잎의 열수추출물 및 에탄올추출물에서 쿼서틴 3-
O -α- L-람노시드, 캠페롤 3-
O -α- L-람노피라노시드, 캠페롤 3,7-
O -α- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드] 등의 천연항산화 활성물질이 존재함을 발견하였다. 즉, 헛개나무의 줄기와 잎을 열수로 추출하여 얻어진 열수추출물을 동결건조한 다음, 메탄올로 용해하여 얻어진 메탄올 가용 추출물을 다양한 컬럼 크로마토그라피 및 HPLC 등의 단계를 거쳐 쿼서틴 3-
O -α- L-람노시드 및 캠페롤 3-
O -α- L-람노시드를 분리하였다. 또 헛개나무 줄기와 잎의 에탄올 추출물을 용매분획하여 얻어진 부탄올 추출물로부터 캠페롤 3,7-
O -a- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드]를 분리하였다. 분리된 물질의 구조결정은 MS, NMR 분석에 의해 이루어졌으며, 분리된 물질은 항산화 활성을 갖고 있음이 확인되었다. 천연항산화 물질을 포함하는 헛개나무 줄기와 잎의 추출물은 산화에 의한 질병예방 및 노화억제 효력을 갖는 기능성 소재로서의 용도를 제공한다.

公开(公告)号：KR1020040087818A

公开(公告)日：2004-10-15

申请号：KR1020030022810

申请日：2003-04-08

Applicant: 박근형

Inventor： 박근형 ,  국주희 ,  문제학 ,  마승진

IPC: C07D311/62

Abstract: PURPOSE: An antioxidative compound isolated from fruits of Catalpa ovata G. Don and an isolation method thereof are provided. The compound has improved antioxidative activity and may be used as a lead compound of other active materials. CONSTITUTION: The antioxidative compound isolated from fruits of Catalpa ovata G. Don is 7-olefinal-3,4'-dihydroxy-3',5-dimethoxyflavane. The method for isolating 7-olefinal-3,4'-dihydroxy-3',5-dimethoxyflavane comprises the steps of: dipping fruits of Catalpa ovata G. Don in methanol, and concentrating the methanol extract under reduced pressure to obtain methanol removed extract; adding a buffer solution with pH 8.0 and ethylacetate into the extract and fractionating the extract into a water-soluble fraction and ethylacetate-soluble neutral fraction; subjecting the ethylacetate soluble neutral fraction to silica gel adsorption column chromatography using chloroform-methanol to obtain an active fraction; subjecting the active fraction to silica gel adsorption column chromatography using ethylacetate-methanol to obtain an active fraction; subjecting the active fraction to silica gel adsorption column chromatography using hexane-ethylacetate to obtain an active fraction; subjecting the active fraction to Sephadex LH-20 column chromatography using methanol-chloroform to obtain an active fraction; subjecting the active fraction to octadecylsilane column chromatography using water-methanol to obtain an active fraction; and subjecting the active fraction to reverse phase-HPLC using methanol-water.

Abstract translation: 目的：提供从枸杞子果实中分离的抗氧化化合物及其分离方法。 该化合物具有改善的抗氧化活性，可用作其它活性物质的铅化合物。 构成：从梓草果实中分离的抗氧化化合物G.Ton是7-烯丙基-3,4'-二羟基-3'，5-二甲氧基黄烷。 分离7-烯丙基-3,4'-二羟基-3'，5-二甲氧基黄烷的方法包括以下步骤：将枸杞子的瓜果浸入甲醇中，减压浓缩甲醇提取物，得到甲醇去除的提取物 ; 向提取物中加入pH 8.0的缓冲溶液和乙酸乙酯，并将提取物分馏成水溶性级分和乙酸乙酯可溶性中性馏分; 使用氯仿 - 甲醇使乙酸乙酯可溶性中性部分进行硅胶吸附柱层析，得到活性部分; 使用乙酸乙酯 - 甲醇使活性级分进行硅胶吸附柱层析，得到活性级分; 使用己烷 - 乙酸乙酯使活性级分进行硅胶吸附柱层析，得到活性级分; 使用甲醇 - 氯仿将活性级分进行Sephadex LH-20柱层析，得到活性级分; 使用水 - 甲醇使活性部分进行十八烷基硅烷柱色谱，得到活性部分; 并使用甲醇 - 水对活性部分进行反相HPLC。

公开(公告)号：KR100388357B1

公开(公告)日：2003-06-19

申请号：KR1020000072613

申请日：2000-12-01

Applicant: 박근형

Inventor： 박근형 ,  조정용 ,  문제학 ,  최기현

IPC: C07D213/62

Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.

Abstract translation: 用途：提供一种从枳st leaf和叶中分离出的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗微生物物质hovenidine B6。 本发明的新颖天然抗菌物质Hovenidine B6是通过以下步骤从枳st st和叶中分离出来的：热榨枳st st和叶; 离心提取物并冷冻干燥上清液以获得提取物粉末; 减压浓缩甲醇滤液得到甲醇提取物; 向甲醇提取液中加入弱碱缓冲液和乙酸乙酯，分液体部分和乙酸乙酯中性部分，向液体部分中加入乙酸乙酯，得到乙酸乙酯酸部分; 使用乙酸乙酯 - 甲醇溶液将上述级分进行硅胶吸附柱色谱法以获得活性级分; 用Sephadex LH-20柱对活性部分进行凝胶过滤层析; 并使用甲醇 - 水溶剂使纯化级分进行反相HPLC以分离Hovenidine B6。

公开(公告)号：KR1020010090122A

公开(公告)日：2001-10-18

申请号：KR1020000014753

申请日：2000-03-23

Applicant: 박근형

Inventor： 박근형 ,  문제학 ,  마승진

IPC: C07C50/02

Abstract: PURPOSE: A novel natural antibacterial compound separated from the root of Pulsatilla Koreana Nakai and its separation method are provided, wherein the compound is named after pulsaquinone and can be used as a lead compound for preparing the various active material. CONSTITUTION: The antibacterial compound named pulsaquinone is represented by the formula 1. The separation method comprises the steps of: vacuum-evaporating the methanol extract of the dry root of Pulsatilla Koreana Nakai to obtain the extract to collect the aqueous part; partitioning the extract with weak alkali buffer and ethyl acetate; controlling the aqueous part to be weak acidic; partitioning the aqueous part with ethyl acetate; eluting the active partition from the ethyl acetate extract by the silica gel adsorption chromatography with ethyl acetate and methanol with increasing the content of methanol gradually; eluting the active material from the active partition by the silica gel adsorption chromatography with ethyl acetate and chloroform; and obtaining pulsaquinone from the active material by carrying out the Sephadex LH-20 chromatography, the silica gel partition column chromatography, the reverse HPLC using methanol-water gradient system and the reverse HPLC in order.

Abstract translation: 目的：提供从白头翁根中分离的新型天然抗菌化合物及其分离方法，其中化合物以脉络嗪命名，可用作制备各种活性物质的铅化合物。 构成：称为脉络洛芬的抗菌化合物由式1表示。分离方法包括以下步骤：真空蒸发白芥白粉干根的甲醇提取物，得到提取物收集含水部分; 用弱碱缓冲液和乙酸乙酯分配提取物; 控制水性部分为弱酸性; 用乙酸乙酯分配水性部分; 用乙酸乙酯和甲醇通过硅胶吸附色谱法从乙酸乙酯萃取液中洗脱活性分配物，逐渐增加甲醇含量; 通过硅胶吸附色谱法用乙酸乙酯和氯仿从活性隔板中洗脱活性物质; 通过进行Sephadex LH-20色谱，硅胶分配柱层析，反相HPLC，使用甲醇 - 水梯度系统和反相HPLC，从活性物质中获得脉冲醌。