公开(公告)号：KR1020040048783A

公开(公告)日：2004-06-10

申请号：KR1020020077531

申请日：2002-12-04

Applicant: 박근형

Inventor： 박근형 ,  김성호 ,  서지우 ,  조정용 ,  장미영 ,  문영환

IPC: G01N33/14

Abstract: PURPOSE: A discriminating method of Jindo-Hongju pigment is provided, thereby easily and accurately discriminating whether red pigment of Jindo-Hongju is derived from puccoon (Lithospermum erythrorhizon Sieb. et Zucc), and maintaining constant quality of Jindo-Hongju pigment. CONSTITUTION: The discriminating method of Jindo-Hongju pigment comprises the steps of: mixing Jindo-Hongju pigment with ethylacetate-hexane mixed solvent in a same ratio, or mixing Jindo-Hongju pigment diluted with 10 to 20% of water with ethyl acetate in a same ratio, settling the mixture for a certain time, and observing color of an upper organic layer with the naked eye; determining whether the Jindo-Hongju pigment has a peak with the highest optical density value at 520 nm, and peaks with second and third optical density values at 496 nm and 550 nm using a spectrophotometer; and measuring the elution pattern by subjecting the Jindo-Hongju pigment to HPLC using the octadecylsilane column.

Abstract translation: 目的：提供金道红菊色素的辨别方法，从而容易准确地区分金杜红色素是否源于茯苓（Lithospermum erythrorhizon Sieb。et Zucc），并保持金道红菊色素的质量。 构成：金道红菊颜料的鉴别方法包括以下方法：将金杜红色素与乙酸乙酯 - 己烷混合溶剂以相同的比例混合，或将乙酸乙酯稀释10〜20％的金杜红色素 相同比例，将混合物沉降一定时间，并用肉眼观察上部有机层的颜色; 确定Jindo-Hongju颜料是否具有在520nm处具有最高光密度值的峰值，并且使用分光光度计在496nm和550nm处具有第二和第三光密度值的峰值; 并使用十八烷基硅烷柱对金杜红菊颜料进行HPLC测定。

公开(公告)号：KR1020020076980A

公开(公告)日：2002-10-11

申请号：KR1020010017769

申请日：2001-03-29

Applicant: 박근형

Inventor： 박근형 ,  조정용 ,  문제학 ,  최기현

IPC: A61K36/72 ,  A61P39/06

Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.

Abstract translation: 目的：一种天然的抗氧化物质，含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷， Ho ia is 其被预期用作具有抗衰老功能的食品和药物的材料。 构成：枸杞子的叶子和茎。 在水中萃取，冷冻干燥，然后在甲醇中萃取，随后进行溶剂分馏，得到乙酸乙酯酸性馏分和神经级分。 然后用纯化方法如硅胶柱色谱，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化提取物中所含的活性物质。

公开(公告)号：KR1020050105910A

公开(公告)日：2005-11-08

申请号：KR1020040032030

申请日：2004-05-03

Applicant: 박근형

Inventor： 박근형 ,  조정용 ,  장미영 ,  문제학 ,  최기현

IPC: A61K31/7048 ,  A61K31/522 ,  A61K36/13

CPC classification number: A61K31/7048 ,  A61K31/522 ,  A61K36/72 ,  A61K2236/33 ,  A61K2236/51

Abstract: 헛개나무(
Hovenia dulcis Thunb) 줄기와 잎의 열수추출물 및 에탄올추출물에서 쿼서틴 3-
O -α- L-람노시드, 캠페롤 3-
O -α- L-람노피라노시드, 캠페롤 3,7-
O -α- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드] 등의 천연항산화 활성물질이 존재함을 발견하였다. 즉, 헛개나무의 줄기와 잎을 열수로 추출하여 얻어진 열수추출물을 동결건조한 다음, 메탄올로 용해하여 얻어진 메탄올 가용 추출물을 다양한 컬럼 크로마토그라피 및 HPLC 등의 단계를 거쳐 쿼서틴 3-
O -α- L-람노시드 및 캠페롤 3-
O -α- L-람노시드를 분리하였다. 또 헛개나무 줄기와 잎의 에탄올 추출물을 용매분획하여 얻어진 부탄올 추출물로부터 캠페롤 3,7-
O -a- L-디람노시드, 카페인, 캠페롤 3-
O -α- L-람노실(1→6)-
O -β- D-글루코실(1→2)-
O -β- D-글루코시드, 그리고 캠페롤 3-
O -α- L-람노시드-7-
O -[β- D-글루코실-(1→3)-α- L-람노시드]를 분리하였다. 분리된 물질의 구조결정은 MS, NMR 분석에 의해 이루어졌으며, 분리된 물질은 항산화 활성을 갖고 있음이 확인되었다. 천연항산화 물질을 포함하는 헛개나무 줄기와 잎의 추출물은 산화에 의한 질병예방 및 노화억제 효력을 갖는 기능성 소재로서의 용도를 제공한다.

公开(公告)号：KR1020040041475A

公开(公告)日：2004-05-17

申请号：KR1020020070532

申请日：2002-11-11

Applicant: 박근형

Inventor： 박근형 ,  김성호 ,  서지우 ,  조정용

IPC: A23L2/04

Abstract: PURPOSE: An enzyme treated welsh onion extract composition containing a natural antioxidant active material obtained by enzymatic treatment of Welsh onion(Allium fistulosum) and then hot air drying or freeze drying thereof is provided. It can be effectively used as an antioxidant functional material having disease preventing and aging inhibiting function. CONSTITUTION: A Welsh onion extract is subjected to an enzymatic treatment with Celluclast(Novo Co.) and Viscozyme(Novo Co.) and then hot air-dried or freeze-dried. The Welsh onion extract composition contains an antioxidant material such as 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, 3,4-dihydroxycinnamic acid having antioxidant activity.

Abstract translation: 目的：提供含有通过酶处理威尔士葱（葱属葱）获得的天然抗氧化活性物质，然后热空气干燥或冷冻干燥的酶处理的威尔士洋葱提取物组合物。 它可以有效地用作具有预防疾病和衰老抑制功能的抗氧化功能材料。 构成：将威尔士洋葱提取物用Celluclast（Novo Co.）和Viscozyme（Novo Co.）进行酶处理，然后热空气干燥或冷冻干燥。 威尔士洋葱提取物组合物含有抗氧化剂，如4-羟基苯甲酸，4-羟基-3-甲氧基苯甲酸，3,4-二羟基苯甲酸，3,4,5-三羟基苯甲酸，具有抗氧化活性的3,4-二羟基肉桂酸 。

公开(公告)号：KR100388357B1

公开(公告)日：2003-06-19

申请号：KR1020000072613

申请日：2000-12-01

Applicant: 박근형

Inventor： 박근형 ,  조정용 ,  문제학 ,  최기현

IPC: C07D213/62

Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.

Abstract translation: 用途：提供一种从枳st leaf和叶中分离出的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗微生物物质hovenidine B6。 本发明的新颖天然抗菌物质Hovenidine B6是通过以下步骤从枳st st和叶中分离出来的：热榨枳st st和叶; 离心提取物并冷冻干燥上清液以获得提取物粉末; 减压浓缩甲醇滤液得到甲醇提取物; 向甲醇提取液中加入弱碱缓冲液和乙酸乙酯，分液体部分和乙酸乙酯中性部分，向液体部分中加入乙酸乙酯，得到乙酸乙酯酸部分; 使用乙酸乙酯 - 甲醇溶液将上述级分进行硅胶吸附柱色谱法以获得活性级分; 用Sephadex LH-20柱对活性部分进行凝胶过滤层析; 并使用甲醇 - 水溶剂使纯化级分进行反相HPLC以分离Hovenidine B6。