公开(公告)号：KR1020140013390A

公开(公告)日：2014-02-05

申请号：KR1020120080204

申请日：2012-07-23

Applicant: 아주대학교산학협력단

Inventor： 심우영 ,  이광 ,  백만정 ,  김재호 ,  현상미 ,  최기령 ,  박성수 ,  강진석 ,  심점순 ,  뉴엔덕도안

IPC: G01N33/00 ,  C12Q1/02 ,  G01N33/48

Abstract: The present invention relates to a method for evaluating toxicity of nanoparticles, and more particularly, to a method for evaluating toxicity of nanoparticles comprising the steps of: obtaining a sample of tissues or cells of a mammal exposed to nanoparticles; irradiating at least one selected from a group consisting of a glutamic acid content, a pyruvate content, whether mitochondria within a cell is damages or not, a reactive oxygen species (ROS) content and an ATP content within the sample; comparing with a control group. The method for evaluating toxicity of nanoparticles according to the present invention analyzes factors having high a correlation with the toxicity of nanoparticles. By using this, it is possible to more precisely detect the toxicity of nanoparticles with excellent detection sensitivity, and through this, it can be effectively used in monitoring or evaluating harmfulness of the toxicity of nanoparticles, as well as effectively used as a tool for examining various diseases, the effects on health or the like when exposed to nanoparticles.

Abstract translation: 本发明涉及一种用于评价纳米颗粒的毒性的方法，更具体地，涉及一种用于评估纳米颗粒毒性的方法，包括以下步骤：获得暴露于纳米颗粒的哺乳动物的组织或细胞样品; 照射来自由谷氨酸含量，丙酮酸含量组成的组中的至少一种，细胞内的线粒体是否损伤，样品中的活性氧（ROS）含量和ATP含量; 与对照组比较。 根据本发明的纳米颗粒的毒性评估方法分析与纳米颗粒的毒性高度相关的因素。 通过使用这种方法，可以更精确地检测具有优异检测灵敏度的纳米颗粒的毒性，通过这种方法，可以有效地用于监测或评估纳米颗粒毒性的有害性，并有效地用作检查的工具 各种疾病，当暴露于纳米颗粒时对健康等的影响。

公开(公告)号：KR101171774B1

公开(公告)日：2012-08-06

申请号：KR1020090116465

申请日：2009-11-30

Applicant: 아주대학교산학협력단

Inventor： 김재호 ,  이광 ,  이재혁 ,  심우영

IPC: C12M3/00 ,  C12N11/02 ,  C12N5/07

Abstract: 줄기세포를 배양하는 지지체가 개시된다. 본 발명에 따른 망상구조체 탄소나노튜브 박막을 지지체로 사용하여 줄기세포를 성장 및 분화시키는 방법과 탄소나노튜브의 막 두께와 표면 거칠기를 조절하여 줄기세포의 성장 및 분화를 조절하고, 막 두께와 표면 거칠기를 조절하여 성장 및 분화시키는 줄기세포의 개체수를 조절하고, 상기 요소들을 포함하는 배양 지지체에 관한 것이다.
탄소나노튜브, 중간엽 줄기세포, 랭뮤어-블로젯 방법

公开(公告)号：KR1020110129364A

公开(公告)日：2011-12-01

申请号：KR1020110049772

申请日：2011-05-25

Applicant: 아주대학교산학협력단

Inventor： 김재호 ,  이광 ,  심우영 ,  이재혁 ,  이민수 ,  강원석

IPC: B82B3/00 ,  H01L21/027

CPC classification number: B82B3/0004 ,  B82B3/0009 ,  G03F7/165 ,  H01L21/0274

Abstract: PURPOSE: A method for forming patterns using a carbon nano-tube langmuir-blodgett(LB) thin film and the growth and differentiation control of stem cells using the same are provided to pattern the carbon nano-tube LB thin film on a substrate using a micro-contact printing method or a lift-off method. CONSTITUTION: A method for forming patterns using a carbon nano-tube LB thin film includes the following: A carbon nano-tube LB thin film is patterned on a substrate using a micro-contact printing method or a lift-off method. The micro-contact printing method transfers the carbon nano-tube LB thin film to a patterned mold. The transferred thin film is in contact with a substrate in order to transfer the patterned thin film to the substrate. The lift-off method transfers entire carbon nano-tube LB thin film to the substrate and to a patterned mold. The transferred thin film is lifted off from the substrate. Stem cells are cultivated on the patterns of the substrate.

Abstract translation: 目的：提供一种使用碳纳米管薄膜（LB）薄膜形成图案的方法，并使用该方法对使用其的干细胞进行生长和分化控制，以使用碳纳米管LB薄膜 微接触印刷法或剥离法。 构成：使用碳纳米管LB薄膜形成图案的方法包括以下：使用微接触印刷法或剥离法将碳纳米管LB薄膜图案化在基板上。 微接触印刷法将碳纳米管LB薄膜转印到图案化的模具上。 转印的薄膜与基底接触，以将图案化的薄膜转移到基底上。 剥离方法将整个碳纳米管LB薄膜转移到基底和图案化的模具上。 将转移的薄膜从衬底上提起。 干细胞在基质的图案上培养。