公开(公告)号：KR1020040087356A

公开(公告)日：2004-10-14

申请号：KR1020030021537

申请日：2003-04-07

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이문희 ,  노영민 ,  최수준 ,  방옥선 ,  정진하

IPC: C12N9/64

CPC classification number: C12N9/6472 ,  C12N15/74 ,  C12Y304/22068

Abstract: PURPOSE: SUMO-specific protease, mUlpB from mouse brain and gene thereof are provided, which are useful for development of anticancer drugs including acute leukemia. CONSTITUTION: The SUMO-specific protease, mUlpB is isolated from mouse brain, wherein the SUMO-specific protease digests protein or peptide bound to SUMO; the SUMO-specific protease has the total or portion of the amino acid sequence of SEQ ID NO:2; and the SUMO-specific protease has molecular weight of 55 kDa. The SUMO-specific protease gene has the total or portion of the nucleotide sequence of SEQ ID NO:1. The expression plasmids pET32a-mUIpB and pCMV-Tag-mUIpB contain the total or portion of the nucleotide sequence of SEQ ID NO:1. A transformant is produced by transforming microorganism BL21 with the expression plasmid pET32a-mUIpB.

Abstract translation: 目的：提供SUMO特异性蛋白酶，来自小鼠脑的mUlpB及其基因，其可用于开发抗癌药物，包括急性白血病。 构成：SUMO特异性蛋白酶mUlpB从小鼠脑中分离，其中SUMO特异性蛋白酶消化与SUMO结合的蛋白质或肽; SUMO特异性蛋白酶具有SEQ ID NO：2的氨基酸序列的全部或部分; 并且SUMO特异性蛋白酶的分子量为55kDa。 SUMO特异性蛋白酶基因具有SEQ ID NO：1的核苷酸序列的全部或部分。 表达质粒pET32a-mUIpB和pCMV-Tag-mUIpB含有SEQ ID NO：1的核苷酸序列的全部或部分。 通过用表达质粒pET32a-mUIpB转化微生物BL21产生转化体。