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公开(公告)号：KR100447535B1
公开(公告)日：2004-09-08
申请号：KR1020020010324
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
Abstract: PURPOSE: A recombinant bacterial system for producing P(3HB-co-3HA) is provided, thereby mass-producing poly(3-hydroxyalkanoate) (PHA) containing a large amount of 3HB having similar properties to the conventional synthetic polymer. CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, beta-ketothiolase synthesizing gene phaA, and NADPH dependent acetoacetyl-CoA reductase synthesizing gene phaB is provided, wherein the recombinant vector is p104613C2ReABstb. A transformant transformed with the recombinant expression vector p104613C2ReABstb is provided, wherein the transformant is Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb). A method for producing P(3HB-co-3HA) comprises culturing a transformant selected from Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb) in a medium containing fatty acid or both fatty acid and carbohydrate.
Abstract translation: 目的：提供一种用于生产P（3HB-共-3HA）的重组细菌系统，由此批量生产含有大量3HB的聚（3-羟基链烷酸酯）（PHA），其具有与常规合成聚合物相似的性质。 构成：提供了含有PHA合成基因phaC，β-酮硫解酶合成基因phaA和NADPH依赖性乙酰乙酰-CoA还原酶合成基因phaB的重组表达载体，其中重组载体是p104613C2ReABstb。 提供了用重组表达载体p104613C2ReABstb转化的转化体，其中转化体是大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）。 一种P（3HB-co-3HA）的制造方法，其特征在于，在含有脂肪酸或脂肪酸和脂肪酸的培养基中培养选自大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）的转化体 糖类。
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公开(公告)号：KR100680132B1
公开(公告)日：2007-02-07
申请号：KR1020040032278
申请日：2004-05-07
Applicant: 한국과학기술원
Abstract: 본 발명은 카르복실기가 노출된 탄소나노튜브(carbon nanotubes; CNT)에 자기성 물질을 결합시키고, 상기 자기성 물질이 고정된 CNT를 기질 상에 위치시킨 다음, 수직 또는 수평 방향으로 자기장을 인가하여 CNT를 기질상에 어레이하는 것을 특징으로 하는 수직 또는 수평으로 정렬된 CNT 어레이의 제조방법에 관한 것이다. 본 발명은 또한, 상기의 CNT 어레이에 바이오 리셉터를 결합시키는 것을 특징으로 하는 CNT-바이오칩의 제조방법 및 이에 의해 제조된 CNT-바이오칩을 이용하는 것을 특징으로 하는 바이오 리셉터와 결합하거나 반응하는 표적 바이오물질 또는 유기화합물의 검출방법에 관한 것이다.
본 발명에 따르면, 종래의 화학합성방법(chemical vapor deposition)에 의한 성장 또는 마이크로 패턴(micro pattern)에 의한 어레이 방법에 비하여 결함이 적으면서도 고도로 CNT를 수직 또는 수평 방향으로 어레이할 수 있다.
탄소나노튜브(CNT), 어레이, 자기장, 바이오칩, 검출방법, 바이오물질-
公开(公告)号：KR100481390B1
公开(公告)日：2005-04-11
申请号：KR1020030015966
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C07K19/00
Abstract: 본 발명은 폴리하이드록시알카노이트 (PHA) 마이크로스피어에 목적단백질 또는 목적리간드가 부착되어 있는 결합체에 있어서, 목적단백질 또는 목적리간드와 PHA 마이크로스피어 사이에 PHA 분해효소의 기질결합 영역이 링커로 삽입되어 있어 목적단백질 또는 목적리간드가 상기 링커를 통해 PHA 마이크로스피어에 연결되어 있는 결합체에 관한 것이다. 본 발명에서 제공되는 PHA 마이크로스피어의 결합체는 목적단백질-반응단백질간의 반응, 목적단백질-반응리간드간의 반응, 목적리간드-반응단백질간의 반응 또는 목적리간드-반응리간드 간의 반응을 효율적으로 검출하는 데 유용하다.
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Patent Agency Ranking
公开(公告)号：KR1020040081238A
公开(公告)日：2004-09-21
申请号：KR1020030015966
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C07K19/00
CPC classification number: C07K17/02 , C12N9/16 , C12Y301/01
Abstract: PURPOSE: A conjugate of PHA microsphere and target protein or ligand and a method for detecting reactions are provided, thereby stably and high efficiently detecting a reaction between target protein and reaction protein, a reaction between target protein and reaction ligand, a reaction between target ligand and reaction protein or a reaction between target ligand and reaction ligand, and cheaply producing the PHA microsphere. CONSTITUTION: The conjugate of PHA microsphere and target protein or ligand contains a substrate-binding site of PHA depolymerase as a linker between the PHA microsphere and target protein or ligand, wherein the target protein is fused in the substrate-binding site of PHA depolymerase; the substrate-binding site of PHA depolymerase is fused with the C-terminal, N-terminal or intermediate of the N-C terminals of the target protein; the target protein is an enzyme or an antibody; the target ligand is low molecular weight compound, nucleic acid, peptide, carbohydrate, fatty acid or lipid which reacts with protein or peptide; the PHA is polymer, copolymer or polymer blend containing C3-C12 monomer; and the substrate-binding site of PHA depolymerase is derived from Alcaligenes faecalis. The method for detecting reactions comprises using the conjugate of PHA microsphere and target protein or ligand, wherein the reaction is detected by using fluorescence-labeled antibody.
Abstract translation: 目的：提供PHA微球和靶蛋白或配体的缀合物和检测反应的方法，从而稳定高效地检测靶蛋白与反应蛋白之间的反应，靶蛋白与反应配体之间的反应，靶配体之间的反应 和反应蛋白质或靶配体与反应配体之间的反应，并且廉价地生产PHA微球。 构成：PHA微球和靶蛋白或配体的缀合物含有PHA解聚酶的底物结合位点，作为PHA微球和靶蛋白或配体之间的接头，其中靶蛋白融合在PHA解聚酶的底物结合位点中; PHA解聚酶的底物结合位点与靶蛋白的N末端的C-末端，N-末端或中间体融合; 靶蛋白是酶或抗体; 靶配体是与蛋白质或肽反应的低分子量化合物，核酸，肽，碳水化合物，脂肪酸或脂质; PHA是含有C 3 -C 12单体的聚合物，共聚物或聚合物共混物; PHA解聚酶的底物结合位点来源于粪产碱杆菌。 检测反应的方法包括使用PHA微球和靶蛋白或配体的缀合物，其中通过使用荧光标记的抗体检测反应。
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公开(公告)号：KR100447531B1
公开(公告)日：2004-09-08
申请号：KR1020020010325
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
Abstract: PURPOSE: A recombinant bacterial system for producing MCL-PHA is provided, thereby mass-producing medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA). CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, gene fadF, and/or gene fadD is provided, wherein the recombinant vector is p10499613C2. A transformant transformed with the recombinant expression vector p10499613C2 is provided, wherein the transformant is Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2). A method for producing MCL-PHA comprises culturing a transformant selected from Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2) in a medium containing fatty acid.
Abstract translation: 目的：提供用于生产MCL-PHA的重组细菌系统，由此批量生产中等链长聚（3-羟基链烷酸酯）（MCL-PHA）。 构成：提供了含有PHA合成基因phaC，基因fadF和/或基因fadD的重组表达载体，其中所述重组载体是p10499613C2。 提供了用重组表达载体p10499613C2转化的转化体，其中转化体是大肠杆菌WA101（p10499613C2），大肠杆菌WB101（p10499613C2），大肠杆菌WAB101（p10499613C2），大肠杆菌KA101（p10499613C2），大肠杆菌KB101（p10499613C2） 或大肠杆菌KAB101（p10499613C2）。 制备MCL-PHA的方法包括培养选自大肠杆菌WA101（p10499613C2），大肠杆菌WB101（p10499613C2），大肠杆菌WAB101（p10499613C2），大肠杆菌KA101（p10499613C2），大肠杆菌KB101（p10499613C2）或大肠杆菌（Escherichia coli） KAB101（p10499613C2）在含有脂肪酸的培养基中培养。
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公开(公告)号：KR1020040069386A
公开(公告)日：2004-08-06
申请号：KR1020030005730
申请日：2003-01-29
Applicant: 한국과학기술원
IPC: G01N33/543
Abstract: PURPOSE: A protein chip using substrate specificity of polyhydroxyalkanonate depolymerase is provided, thereby inhibiting nonspecific protein binding, so that cost for production of the protein chip can be reduced. CONSTITUTION: The protein chip using substrate specificity of polyhydroxyalkanonate depolymerase comprises a substrate, PHA(polyhydroxyalkanonate) fixed on the substrate, a substrate binding domain of PHA depolymerase, and a fusion protein of a target protein, wherein the substrate is glass slide; the PHA is spin coated on the substrate; the PHA has 4 to 12 of carbon number; the binding domain of PHA depolymerase is fused with the N-terminal, C-terminal or the intermediate of N-C of a target protein; and the binding domain of PHA depolymerase is isolated from Alcaligenes faecalis.
Abstract translation: 目的：提供使用聚羟基链烷酸酯解聚酶的底物特异性的蛋白质芯片，从而抑制非特异性蛋白质结合，从而可以降低生产蛋白质芯片的成本。 构成：使用聚羟基链烷酸酯解聚酶的底物特异性的蛋白质芯片包括固定在底物上的底物PHA（聚羟基卡那酸），PHA解聚酶的底物结合结构域和靶蛋白的融合蛋白，其中所述底物是玻璃载片; 将PHA旋涂在基材上; PHA有4到12个碳数; PHA解聚酶的结合域与靶蛋白的N末端，C-末端或中间体融合; 并且从粪产碱杆菌中分离出PHA解聚酶的结合结构域。
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公开(公告)号：KR1020050051148A
公开(公告)日：2005-06-01
申请号：KR1020030084888
申请日：2003-11-27
Applicant: 한국과학기술원
CPC classification number: B82Y30/00 , G01N33/544
Abstract: 본 발명은 자기조립(self-assembly)물질을 탄소나노튜브 상에 자기조립시키는 것을 특징으로 하는 자기조립물질로 랩핑(wrapping)된 수용성 탄소나노튜브의 제조방법 및 자기조립물질로 랩핑되어 있는 수용성 탄소나노튜브에 관한 것이다.
본 발명에 따른, 자기조립물질로 랩핑된 탄소나노튜브는 수용성 성질을 나타내므로 일반 탄소나노튜브와 비교하여 월등히 우수한 응용성을 가지게 된다. 특히, 상기 자기조립물질로 랩핑된 탄소나노튜브에 표적 바이오물질 혹은 유기화합물과 결합하는 리셉터를 선택적으로 부착하여 바이오센서를 제작하는 것이 가능하다.-
公开(公告)号：KR1020030070789A
公开(公告)日：2003-09-02
申请号：KR1020020010324
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
CPC classification number: C12N15/70 , C12P7/625 , C12Y101/01036 , C12Y203/01016
Abstract: PURPOSE: A recombinant bacterial system for producing P(3HB-co-3HA) is provided, thereby mass-producing poly(3-hydroxyalkanoate) (PHA) containing a large amount of 3HB having similar properties to the conventional synthetic polymer. CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, beta-ketothiolase synthesizing gene phaA, and NADPH dependent acetoacetyl-CoA reductase synthesizing gene phaB is provided, wherein the recombinant vector is p104613C2ReABstb. A transformant transformed with the recombinant expression vector p104613C2ReABstb is provided, wherein the transformant is Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb). A method for producing P(3HB-co-3HA) comprises culturing a transformant selected from Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb) in a medium containing fatty acid or both fatty acid and carbohydrate.
Abstract translation: 目的：提供一种用于生产P（3HB-co-3HA）的重组细菌系统，从而大量生产含有大量与常规合成聚合物具有相似性质的3HB的聚（3-羟基链烷酸酯）（PHA）。 构成：提供了含有PHA合成基因phaC，β-酮硫解酶合成基因phaA和NADPH依赖性乙酰乙酰辅酶A还原酶合成基因phaB的重组表达载体，其中重组载体为p104613C2ReABstb。 提供了用重组表达载体p104613C2ReABstb转化的转化体，其中转化体是大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）。 制备P（3HB-co-3HA）的方法包括在含有脂肪酸或脂肪酸两者的介质中培养选自大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）的转化体， 糖类。
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