公开(公告)号：KR1020000060322A

公开(公告)日：2000-10-16

申请号：KR1019990008512

申请日：1999-03-13

Applicant: 한국과학기술원

Inventor： 이준식 ,  반재구 ,  안정훈

IPC: C12N15/31 ,  C12N15/67

CPC classification number: C07K14/21

Abstract: PURPOSE: A novel method for producing a pseudomonas fluorescence derived gene which promotes exporting of exogenous protein, is provided to give convenience in secreting the protein effectively out of a cell. CONSTITUTION: The gene promoting exporting of exogenous protein is characterized by being derived from pseudomonas fluorescence, and comprising nucleotides of sequence number 4, number 5 and number 6 orderly. In particular, the method thereof comprises identifying the gene which promotes exporting of exogenous protein from pseudomonas fluorescence SIK W1(KCTC 2689P) outwardly to environment rather than the subject cell. Also, an expression vector of the gene, pABC-ACYC, is characterized by having specific gene map, as shown in figure 1. Further, provided is E.coli XL1-Blue/pABC-ACYC (KCTC 0586BP) transformed by the expression vector pABC-ACYC.

Abstract translation: 目的：提供促进外源蛋白质输出的假单胞菌荧光衍生基因的新方法，以便有效地从细胞中分泌蛋白质。 构成：促进外源蛋白质输出的基因的特征在于衍生自假单胞菌荧光，其序列号为4，5和6的核苷酸有序。 特别地，其方法包括鉴定促进外源蛋白质从假单胞菌荧光SIK W1（KCTC 2689P）向外输出到环境而不是主体细胞的基因。 此外，基因表达载体pABC-ACYC的特征在于具有特异性基因图，如图1所示。此外，提供了通过表达载体转化的大肠杆菌XL1-Blue / pABC-ACYC（KCTC 0586BP） PABC-ACYC。