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Patent Agency Ranking
公开(公告)号：KR1019910000464B1
公开(公告)日：1991-01-25
申请号：KR1019880016979
申请日：1988-12-19
Applicant: 주식회사 삼양제넥스 , 한국과학기술원
IPC: C12P21/00
Abstract: Single cell protein(SCP) was produced by continously culturing a yeast under aerobic condition. The growth rate of yeast is decreased gradually by continously feeding aq. medium to a fermenter. The growth of yeast is 0.35-0.20/hr for the easly fermentation period (ohr. to 14hr), 0.20-0.07/hr fer the middle period (14-20hr), 0.07-0.03/hr for the later period (20-28hr). The process reduces the cost for producing single cell protein.
Abstract translation: 单细胞蛋白（SCP）是在有氧条件下连续培养酵母产生的。 酵母菌的生长速度逐渐下降。 中等至发酵罐。 发酵时间（ohr。至14hr），中期（14-20hr）为0.20-0.07 / hr，后期（20-28hr）为0.07-0.03 / hr，酵母的生长为0.35-0.20 ）。 该方法降低了生产单细胞蛋白质的成本。
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公开(公告)号：KR100312456B1
公开(公告)日：2001-11-03
申请号：KR1019990008512
申请日：1999-03-13
Applicant: 한국과학기술원
CPC classification number: C07K14/21
Abstract: 본발명은미생물에서외래단백질을발현시켜세포밖 배양배지로효율적으로분비시킬수 있도록하는단백질을코딩하는슈도모나스플루오레슨스에서유래한분비촉진유전자, 그를포함하는재조합발현벡터와, 전기발현벡터에의하여형질전환된형질전환체, 및전기발현벡터로형질전환된미생물을배양하고외래단백질의발현을유도하여, 발현된외래단백질이세포밖 배양액으로분비되도록유도하는단계를포함하는외래단백질의생산방법에관한것이다. 본발명에따라일반분비경로에비하여훨씬간단하고효과적으로외래단백질을세포밖으로분비하게할 수있는분비촉진유전자에의한분비시스템이새롭게개발되었으며, 특히슈도모나스플루오레슨스에서외래단백질을세포밖으로분비시키는기능을담당하는분비촉진유전자를규명함으로써, 이분비시스템을이용하여외래단백질을효율적으로발현시키고, 이를산업적으로다양하게이용하는목적에유용하게사용할수 있다.
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公开(公告)号：KR1020000060322A
公开(公告)日：2000-10-16
申请号：KR1019990008512
申请日：1999-03-13
Applicant: 한국과학기술원
CPC classification number: C07K14/21
Abstract: PURPOSE: A novel method for producing a pseudomonas fluorescence derived gene which promotes exporting of exogenous protein, is provided to give convenience in secreting the protein effectively out of a cell. CONSTITUTION: The gene promoting exporting of exogenous protein is characterized by being derived from pseudomonas fluorescence, and comprising nucleotides of sequence number 4, number 5 and number 6 orderly. In particular, the method thereof comprises identifying the gene which promotes exporting of exogenous protein from pseudomonas fluorescence SIK W1(KCTC 2689P) outwardly to environment rather than the subject cell. Also, an expression vector of the gene, pABC-ACYC, is characterized by having specific gene map, as shown in figure 1. Further, provided is E.coli XL1-Blue/pABC-ACYC (KCTC 0586BP) transformed by the expression vector pABC-ACYC.
Abstract translation: 目的：提供促进外源蛋白质输出的假单胞菌荧光衍生基因的新方法，以便有效地从细胞中分泌蛋白质。 构成：促进外源蛋白质输出的基因的特征在于衍生自假单胞菌荧光，其序列号为4，5和6的核苷酸有序。 特别地，其方法包括鉴定促进外源蛋白质从假单胞菌荧光SIK W1（KCTC 2689P）向外输出到环境而不是主体细胞的基因。 此外，基因表达载体pABC-ACYC的特征在于具有特异性基因图，如图1所示。此外，提供了通过表达载体转化的大肠杆菌XL1-Blue / pABC-ACYC（KCTC 0586BP） PABC-ACYC。
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