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公开(公告)号：KR101408239B1
公开(公告)日：2014-06-17
申请号：KR1020110084880
申请日：2011-08-25
Applicant: 한국에너지기술연구원
Abstract: 본 발명은 미세조류 광생물반응기 및 이를 이용한 미세조류 생산방법에 관한 것으로, 보다 상세하게는 미세조류 및 배양액을 수용하기 위한 비닐백을 연속 공급하여 미세조류의 배양 및 회수를 용이하게 할 수 있는 미세조류 광생물반응기 및 이를 이용한 미세조류 생산방법에 관한 것이다.
본 발명에 따른 미세조류 광생물반응기는, 지면으로부터 일정높이 이격 설치된 걸이대에 연속공급이 가능한 비닐백이 설치되고 상기 비닐백에서 인출된 비닐백 하부에는 이산화탄소공급부와 산소배출부가 형성된 밀폐수단이 결합되는 것을 특징으로 한다.-
公开(公告)号：KR1020140042411A
公开(公告)日：2014-04-07
申请号：KR1020120109148
申请日：2012-09-28
Applicant: 한국에너지기술연구원
Abstract: A vinyl sheet type photobio-reactor having a spout is disclosed. The vinyl sheet type photobio-reactor having a spout comprises: a reacting sheet where a culture space to accommodate photo-bio organisms and a culture medium is formed and a plurality of penetration units are formed as a front and a rear surface are adhered; and a gas supply unit for supplying gas into the culture space of the reaction sheet. The gas supply unit comprises a spout which is interposed to the front and the rear surface of the reacting sheet thereby being combined to the reacting sheet.
Abstract translation: 公开了一种具有喷嘴的乙烯基片式光电反应器。 具有喷口的乙烯基类型光生反应器包括：反应片，其中形成培养空间以容纳光生物生物体和培养基，并且形成多个穿透单元作为前表面和后表面; 以及用于将气体供给到反应片的培养空间中的气体供给单元。 气体供给单元包括插入反应片的前表面和后表面的喷口，从而与反应片组合。
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公开(公告)号：KR1020130120019A
公开(公告)日：2013-11-04
申请号：KR1020120042958
申请日：2012-04-25
Applicant: 한국에너지기술연구원
Abstract: In order to effectively extract bio-oil from raw materials which can be used for manufacturing biodiesel, less moisture improves oil extraction efficiency. However, in order to remove moisture from oil containing microorganism, additional processing costs are required, so a new oil extracting method of which the oil extraction efficiency is excellent is required. The present invention relates to a bio-oil manufacturing method capable of extracting moistened oil comprising the following steps: adding clay into a culture medium including oil containing microorganism and depositing the microorganism; adding peroxides into the deposited microorganism and degrading cell walls; and recovering an oil layer discharged from the microorganism. According to the bio-oil manufacturing method capable of extracting moistened oil, the high efficiency extraction of bio-oil which is a raw material for biodiesel from oil containing microorganism is possible, and the method can be utilized in a manufacturing process of fatty acid such as omega-3 fatty acid containing DHA and EPA. [Reference numerals] (AA) Oil extract efficiency (%);(BB) Peroxide concentration (%)
Abstract translation: 为了有效提取可用于生产生物柴油的原料的生物油，较少的水分提高了油提取效率。 然而，为了从含油微生物中除去水分，需要额外的加工成本，因此需要油提取效率优异的新的油提取方法。 本发明涉及一种能够提取润湿油的生物油制造方法，包括以下步骤：将粘土添加到包含含微生物的培养基中并沉积微生物; 向沉积的微生物中加入过氧化物并降解细胞壁; 并回收从微生物排出的油层。 根据能够提取润湿油的生物油制造方法，可以从作为含油微生物的生物柴油的原料中高效提取生物油，该方法可用于脂肪酸的制造过程 作为含有DHA和EPA的ω-3脂肪酸。 （AA）油提取效率（％）;（BB）过氧化物浓度（％）
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公开(公告)号：KR101315886B1
公开(公告)日：2013-10-15
申请号：KR1020110084881
申请日：2011-08-25
Applicant: 한국에너지기술연구원
Abstract: 본 발명은 다면체로 이루어진 광생물반응기에 관한 것으로, 보다 상세하게는 비닐 재질의 시트(sheet)를 이용하여 다면체 구조로 형성함으로써 빛의 조사 면적을 향상시키는 다면체로 이루어진 광생물반응기에 관한 것이다.
본 발명에 따른 다면체로 이루어진 광생물반응기는, 외부에 일정 높이로 다수개의 고정수단을 서로 교호되게 고정시켜 상,하 및 좌,우로 각각의 삼각형상으로 상호 대칭되게 형성되며 그 내부에 미세조류 및 배양액이 수용되는 반응시트와; 상기 반응시트의 내부에 이산화탄소를 공급하는 이산화탄소공급부와; 상기 반응시트의 내부로 공급되었던 이산화탄소에서 미세조류의 광합성작용에 의해 분해된 산소를 배출시키는 산소배출부를 포함하여 구성되는 것을 특징으로 한다.-
公开(公告)号：KR1020130023854A
公开(公告)日：2013-03-08
申请号：KR1020110086914
申请日：2011-08-30
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: Chlorella N113 with an excellent carbon dioxide fixation ability is provided to effectively remove carbon dioxide and to produce biodiesel. CONSTITUTION: A method for fixing carbon dioxide comprises a step of fixing carbon dioxide contained in coal-fired exhaust gas into Chlorella sp. N113. The coal-fired exhaust gas contains 10-20 wt% of carbon dioxide, 100-300 ppm of CO, 150-350 ppm of SOX, 150-350 ppm of NOX, and 5-15 mg/m^3 of dust. [Reference numerals] (AA) Air(0.035%); (BB) Synthetic CO_2(10%); (CC) Coal exhaust gas(CO_2 ~15%); (DD) KCTC strain 46 kinds + KR-1; (EE) KCTC strain 24 kinds + KR-1; (FF) KCTC strain 16 kinds + KR-1; (GG) Strain with superior CO_2 and temperature resistance
Abstract translation: 目的：提供具有优异二氧化碳固定能力的小球藻N113，有效去除二氧化碳并生产生物柴油。 构成：固定二氧化碳的方法包括将包含在燃煤废气中的二氧化碳固定在小球藻中的步骤。 N113。 燃煤废气含有10-20重量％的二氧化碳，100-300ppm的CO，150-350ppm的SOX，150-350ppm的NOX和5-15mg / m 3的粉尘。 （标号）（AA）空气（0.035％）; （BB）合成CO_2（10％）; （CC）煤废气（CO_2〜15％）; （DD）KCTC菌株46种+ KR-1; （EE）KCTC菌株24种+ KR-1; （FF）KCTC菌株16种+ KR-1; （GG）具有优异CO_2和耐温性的菌株
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Patent Agency Ranking
公开(公告)号：KR1020130022112A
公开(公告)日：2013-03-06
申请号：KR1020110084879
申请日：2011-08-25
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A photo-bioreator with a carbon dioxide mixing and guiding device, and a method for culturing microalgae using the same are provided to enhance photosynthesis efficiency by activating the mixture of carbon dioxide with the microalgae in a chamber. CONSTITUTION: A photo-bioreactor with a carbon dioxide mixing and guiding device comprises a chamber(1), a carbon dioxide supply unit(2), an oxygen discharge unit(3), and the carbon dioxide mixing and guiding device. The chamber has a cover(11) at the upper portion, and contains microalgae and a culture medium. The carbon dioxide supply unit supplies carbon dioxide into the chamber. The oxygen discharge unit discharges oxygen generated from the carbon dioxide inside the chamber through the photosynthesis of the microalgae. The carbon dioxide mixing and guiding device induces the mixture of the carbon dioxide and the microalgae.
Abstract translation: 目的：提供一种具有二氧化碳混合和引导装置的光生物反应器，以及使用其的微藻培养方法，以通过在室中活化二氧化碳与微藻的混合物来提高光合作用效率。 构成：具有二氧化碳混合引导装置的光生物反应器包括室（1），二氧化碳供应单元（2），氧气排放单元（3）和二氧化碳混合引导装置。 该室在上部具有盖（11），并且包含微藻和培养基。 二氧化碳供应单元向室提供二氧化碳。 氧气放电单元通过微藻的光合作用来释放室内二氧化碳产生的氧气。 二氧化碳混合引导装置引起二氧化碳和微藻的混合物。
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公开(公告)号：KR1020120054973A
公开(公告)日：2012-05-31
申请号：KR1020100116397
申请日：2010-11-22
Applicant: 한국에너지기술연구원
CPC classification number: C10L5/44 , C10B53/02 , C10B57/02 , C10L1/02 , C10L9/083 , C11B1/02 , Y02E50/10 , Y02E50/14 , Y02E50/15 , Y02E50/30
Abstract: PURPOSE: A collecting method of lipids from microalgae is provided to effectively collect lipids among various components in microalgae by simple processes, and to manufacture a part of pure hydrocarbon consisting of only carbon and hydrogen by decomposing lipids during thermal decomposition. CONSTITUTION: A collecting method of lipids from microalgae comprises a step of primary thermal-decomposing microalgae biomass at 250-400 °C; a step of removing saccharides and proteins from the microalgae biomass; and a step of obtaining primary decomposed product; a step of secondary decomposing the primary decomposed product and collecting only lipids, which are able to be converted to fuel easily.
Abstract translation: 目的：提供微藻类脂质的收集方法，通过简单的方法有效收集微藻各种成分之间的脂质，并通过在热分解过程中分解脂质制备一部分仅由碳和氢组成的纯烃。 构成：来自微藻的脂质的收集方法包括在250-400℃下初步热分解微藻生物质的步骤; 从微藻生物量中除去糖类和蛋白质的步骤; 并获得初级分解产物的步骤; 二次分解初级分解产物并仅收集能够容易地转化为燃料的脂质的步骤。
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18.发明公开ｇｌｇＡ１ 유전자와 이를 포함하는 재조합벡터와 이로부터 형질전환된 숙주세포와 이의 생합성 제어를 통한 남세균 총지질 함량 조절 방법 有权糖原合成酶1基因和重组载体，其包含通过糖原合成酶1缺陷型蓝藻综合症PCC 6803的载体和脂肪酸组成和含量的转化的基因和宿主细胞
公开(公告)号：KR1020110045865A
公开(公告)日：2011-05-04
申请号：KR1020090102599
申请日：2009-10-28
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A gene gIgA1 encoding glycogen synthase 1 from cyanobacteria is provided to control total content of cyanobacteria by controlling biosynthesis. CONSTITUTION: A gIgA1 gene contains a base sequence of sequence number 1. A recombinant vector contains the gIgA1 gene relating to glycogen biosynthesis of cyanobateria. A host cell which is transformed by the recombinant vector is cyanobacteria PCC6803. A method for preparing transformed cyanobacteria comprises: a step of extracting chromosomal DNA from cyanobacteria Synechocystis PCC6803 wild type; a step of performing PCT using upstream and downstream of the isolated gIgA1 gene a primer; a step of performing PCR of the PCR product and T vector as a template together with antibiotic reactant; a step of cloning mutant cassette to pTOP V2 blunt vector (Enzynomics, Korea); and a step of transforming Synechocystis extract.
Abstract translation: 目的：通过控制生物合成提供从蓝细菌编码糖原合成酶1的gIgA1基因，以控制蓝细菌的总含量。 构成：gIgA1基因含有序列号1的碱基序列。重组载体含有与青花碱的糖原生物合成有关的gIgA1基因。 由重组载体转化的宿主细胞是蓝细菌PCC6803。 制备转化蓝细菌的方法包括：从蓝细菌集胞藻PCC6803野生型提取染色体DNA的步骤; 在分离的gIgA1基因的上游和下游进行PCT的一个引物; 将PCR产物和T载体作为模板与抗生素反应物一起进行PCR的步骤; 将突变体盒克隆到pTOP V2钝化载体（Enzynomics，Korea）的步骤; 和转化集胞藻提取物的步骤。
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公开(公告)号：KR100794384B1
公开(公告)日：2008-01-15
申请号：KR1020060073104
申请日：2006-08-02
Applicant: 한국에너지기술연구원
IPC: C12N9/02
Abstract: A method for producing and purifying hydrogenase is provided to produce the highly concentrated hydrogenase by culturing Thiocapsa roseopersicina in an acetate-added culture medium and purify the hydrogenase with stable activity from a culture material of the Thiocapsa roseopersicina. A method for producing and purifying hydrogenase comprises the steps of: (a) treating fungi bodies obtained by centrifuging a culture solution obtained by culturing Thiocapsa roseopersicina with ultrasonic wave to lyse the fungi bodies; (b) precipitating the fungi body lysate using 60% ammonium sulfate to obtain an inactive hydrogenase fraction; (c) heat-treating the fraction at a temperature of 55-65 deg.C for 15-25 minutes; (d) subjecting the fraction to an anion exchange chromatography using KCl as a solvent; (e) subjecting the fraction to a hydrophobic interaction chromatography using a 2M ammonium sulfate-added potassium phosphate buffer solution(50mM, pH 7.0); (f) subjecting the fraction to an anion exchange chromatography using 20mM Tris-HCl buffer solution as a solvent; (g) subjecting the fraction to a gel filtration chromatography using a phosphate buffer(50 mMpH 7.0) including 150 mM KCl; and (h) subjecting the fraction to a gel electrophoresis using 10% polyacrylamide gel.
Abstract translation: 提供氢化酶的制备和纯化方法，通过在乙酸盐加入的培养基中培养玫瑰聚糖硫杆菌来生产高度浓缩的氢化酶，并且从硫柳塞氏菌的培养物中纯化具有稳定活性的氢化酶。 一种生产和纯化氢化酶的方法，包括以下步骤：（a）处理通过离心通过用超声波培养玫瑰新西兰薄荷蜡获得的培养液而获得的真菌，以裂解真菌体; （b）使用60％硫酸铵沉淀真菌体裂解液以获得无活性氢化酶级分; （c）在55-65℃的温度下热处理15-25分钟; （d）使用KCl作为溶剂进行阴离子交换层析; （e）使用2M硫酸铵加入的磷酸钾缓冲溶液（50mM，pH7.0）对所述级分进行疏水相互作用层析; （f）使用20mM Tris-HCl缓冲溶液作为溶剂对该级分进行阴离子交换层析; （g）使用包含150mM KCl的磷酸盐缓冲液（50mMpH 7.0）对该级分进行凝胶过滤层析; 和（h）使用10％聚丙烯酰胺凝胶对级分进行凝胶电泳。
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