公开(公告)号：KR1020050055202A

公开(公告)日：2005-06-13

申请号：KR1020030088257

申请日：2003-12-05

Applicant: 한국전자통신연구원

Inventor： 강선길 ,  양해식 ,  신동호 ,  김효겸 ,  김윤태

IPC: G01N27/327

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: 본 발명은 체내삽입 연속측정 바이오센서의 미세 기준전극, 그 제조방법 및 이 기준전극을 이용한 체내삽입형 연속측정 혈당센서에 관한 것으로, 절연기판 상에 형성된 전극용 금속막 및 전극용 금속막 상에 형성된 산화이리듐막을 포함하는 체내삽입형 연속측정 바이오센서의 기준전극 및 그 제조방법을 제공한다. 본 발명에 의하면, 산화이리듐막 기준전극은 제조공정이 간단하며 반도체 일괄공정을 사용하여 제작할 수 있는 효과가 있다.

公开(公告)号：KR1020040046175A

公开(公告)日：2004-06-05

申请号：KR1020020074014

申请日：2002-11-26

Applicant: 한국전자통신연구원

Inventor： 이대식 ,  양해식 ,  고종수 ,  윤태환 ,  김효겸 ,  신용범 ,  김성진 ,  박세호 ,  김규원 ,  김윤태

IPC: C12M1/00 ,  G01N27/26

Abstract: PURPOSE: A low power consumption microfabricated thermal cycler and a method for fabrication of the same are provided, thereby accurately and minutely controlling the temperature of the reaction occurring area, so that it can be applied to various bio chips including PCR chip, protein chip, DNA chip, drug delivery system, micro biological/chemical reactor and lab-on-a-chip. CONSTITUTION: The low power consumption microfabricated thermal cycler comprises an upper board(112) and a lower board(100), wherein the upper board(112) comprises an fluid inlet(114) and outlet, a reaction chamber(118), and a fluid channel(116) connecting the inlet(114) and outlet to the reaction chamber(118); and the lower board(100) comprises an insulated heating thin layer(106) formed on the lower board(100), a heating means(102) formed on the insulated heating thin layer(106), a temperature sensor(104) formed on the insulated heating thin layer(106), and an insulating layer(108) covering the heating means(102) and the temperature sensor(104); and the insulated heating thin layer(106) is composed of Si3N4, SiO2, Si3N4/SiO2/Si3N4 or SiO2/Si3N4/SiO2 and has thickness of 0.1 to 10 micrometer.

Abstract translation: 目的：提供一种低功耗微加热热循环仪及其制造方法，从而准确，精确地控制反应发生区域的温度，从而可应用于各种生物芯片，包括PCR芯片，蛋白芯片， DNA芯片，药物输送系统，微生物/化学反应器和芯片实验室。 构成：低功耗微加热热循环仪包括上板（112）和下板（100），其中上板（112）包括流体入口（114）和出口，反应室（118）和 将入口（114）和出口连接到反应室（118）的流体通道（116）; 并且所述下板（100）包括形成在所述下板（100）上的绝缘加热薄层（106），形成在所述绝缘加热薄层（106）上的加热装置（102），形成在所述绝缘加热薄层 绝缘加热薄层（106）和覆盖加热装置（102）和温度传感器（104）的绝缘层（108）。 绝缘加热薄层（106）由Si3N4，SiO2，Si3N4 / SiO2 / Si3N4或SiO2 / Si3N4 / SiO2组成，厚度为0.1〜10微米。

公开(公告)号：KR1020040035478A

公开(公告)日：2004-04-29

申请号：KR1020020064638

申请日：2002-10-22

Applicant: 한국전자통신연구원

Inventor： 신동호 ,  강선길 ,  김효겸 ,  양해식 ,  김윤태

IPC: G01N33/544

Abstract: PURPOSE: A method for forming an outermost layer of enzymatic electrode sensor is provided, thereby effectively preventing denaturation of an enzyme, so that activity of the enzyme can be maintained. CONSTITUTION: A method for forming an outermost layer of enzymatic electrode sensor consisting of an electrode, an enzyme layer and an outermost layer comprises the steps of: and forming a first layer on the enzyme layer for preventing the enzyme denaturation; forming a second layer prepared by dissolving polymer or monomer in organic solvent, wherein the first layer selectively reacts with the polymer or monomer dissolved in organic solvent to form gel, wherein the polymer is hydrogel; hydrogel is selected from poly(2-hydroxyethyl methacrylate), polyvinyl alcohol, polyacryl amide, poly(N-vinyl 2-pyrrolidone), polyethylene oxide, polyethyleneglycol and polyethylene glycol monomethyl ether; the organic solvent is selected from methanol, ethanol, tetrahydrofuran and N,N-methylformamide; and the monomer is selected from ethylene glycol, hydroxyethyl methacrylate and derivatives thereof.

Abstract translation: 目的：提供形成酶电极传感器的最外层的方法，从而有效地防止酶的变性，从而可以保持酶的活性。 构成：由电极，酶层和最外层构成的酶电极传感器的最外层的方法包括以下步骤：在酶层上形成防止酶变性的第一层; 形成通过将聚合物或单体溶解在有机溶剂中制备的第二层，其中第一层选择性地与溶解在有机溶剂中的聚合物或单体反应形成凝胶，其中聚合物是水凝胶; 水凝胶选自聚（甲基丙烯酸2-羟乙酯），聚乙烯醇，聚丙烯酰胺，聚（N-乙烯基-2-吡咯烷酮），聚环氧乙烷，聚乙二醇和聚乙二醇单甲醚; 有机溶剂选自甲醇，乙醇，四氢呋喃和N，N-甲基甲酰胺; 单体选自乙二醇，甲基丙烯酸羟乙酯及其衍生物。