Abstract:
본 발명은 표면장력으로 제어되는 미세유체소자에 관한 것으로, 상부 기판 및 하부 기판이 결합되어 유체를 제어하고 반응시키는 미세유체소자에 있어서, 하부 기판은, 제1 유체 및 제2 유체가 각각 저장되는 제1 저장 챔버 및 제2 저장 챔버와, 제1 저장 챔버 및 제2 저장 챔버와 연결되는 감지 챔버와, 제1 유체 또는 제2 유체의 이동을 정지시키는 제1 유동 정지부 및 제2 유동 정지부와, 제1 유체 또는 제2 유체의 이동 속도를 감소시키는 유동 지연부와, 반응이 완료된 제1 유체 또는 제2 유체가 폐기되는 폐기 챔버 및 유체가 이동되도록 유동 지연부와 폐기 챔버 사이를 연결하는 유로를 포함하고, 상부 기판은 감지 챔버 내의 생화학반응을 측정하는 감지부를 포함하며, 제1 유체가 모세관 힘에 의해 감지 챔버로 이동하여 1차 생화학 반응이 일어나고, 소정 시간 경과 후 제2 유체가 모세관 힘에 의해 감지 챔버로 유입되면 제1 유체의 교체 및 2 차 생화학반응이 일어나는 것을 특징으로 한다. 따라서, 추가적인 장치 및 전원공급이 필요 없게 되어, 장치의 소형화, 휴대화가 가능하고, 제조비를 낮춤과 동시에 제조 수율을 높일 수 있을 뿐만 아니라, 사용 시 고장이 거의 없는 효과가 있다.
Abstract:
PURPOSE: A microfluidic device applied to a biochip is provided to mix microfluid by disposing hot wires in a reaction chamber to form layers and heating or cooling stored fluids. CONSTITUTION: The microfluidic device for mixing microfluid comprises a first flow channel delivering a first fluid(106); a second flow channel delivering a second fluid(108); a reaction chamber connected to ends of the first and second flow channels and having hot wires(104) for heating the inside; and a part for stopping flow connected to end of the reaction chamber so as to prevent the first and second fluids from moving. When the first and second fluids meet in the reaction chamber and form layers, the first and second fluids are heated or cooled using the hot wires to generate the density difference, thereby mixing the first and second fluids by gravity.
Abstract:
PURPOSE: A fluorescence detecting device for detecting biomolecule using amorphous-silicon thin film transistor and a method for detecting the fluorescence using the same are provided, thereby reducing the size and costs of DNA chip because optical devices are not used, and requiring no fluorescence stimulating light blocking filter by using a transparent substrate. CONSTITUTION: A fluorescence detecting device for detecting biomolecule using amorphous-silicon thin film transistor comprises a first substrate with a probe biomolecule; a transparent substrate(102) having a first domain(I), a second domain(II) and a third domain(III), and having a first surface(102a) where a light is irradiated and a second surface(102b) arranged to face the first substrate; a light thin film transistor(TFToptic) producing electric charge responding to the fluorescence produced from the biomolecule on the second surface(102b) and positioned in the first domain(I); a capacity(CAP) storing the electric charge produced from the light thin film transistor(TFToptic) and positioned in parallel to the light thin film transistor(TFToptic) in the second domain(II); and a transfer thin film transistor(TFTtrans) transferring the electric charge to an additional analysis system and positioned in parallel to the capacity(CAP) in the third domain(III).
Abstract:
PURPOSE: A surface plasmon resonance sensor system is provided to prevent a metal layer from being deformed by forming a transparent thin film on the metal layer and bonding the transparent thin film to the metal layer by using an adhesive layer. CONSTITUTION: A surface plasmon resonance sensor system includes a transparent substrate(13a). A first adhesive layer, a conductive thin film(13b), a second adhesive layer(13d), and a transparent layer(13e) are sequentially deposited on the transparent substrate(13a), thereby forming a sensor section of a sensor chip(13). A sample to be measured is positioned at an upper portion of the sensor chip(13). A prism(12) is attached to a lower portion of the sensor chip(13) in order to create a plasmon resonance by reacting with a surface of the conductive thin film(13b). A light source(11) is provided to supply light to the sensor chip(13) through the prism(12). A light receiving section(14) receives light reflected from the sensor chip(13).
Abstract:
본 발명은, 기판에 부착된 프로브 생체 분자에 분석 대상 생체 분자를 결합시키고, 결합된 생체 분자에 광을 조사하여 발생되는 형광을 검출하는 생체 분자 감지를 위한 형광 검출 소자에 관한 것이다. 이 형광 검출 소자는, 투명 기판, 광 박막 트랜지스터, 커패시터 및 전달 박막 트랜지스터를 포함한다. 투명 기판은, 제1 영역, 제2 영역 및 제3 영역을 가지며, 제1 표면으로 광이 조사되고 제1 표면과 반대의 제2 표면은 제1 기판의 프로브 생체 분자가 부착된 면과 대향하도록 배치된다. 광 박막 트랜지스터는, 제1 영역에서 투명 기판의 제2 표면 위에 배치되어 결합된 생체 분자로부터 발생되는 형광에 대응하는 전하를 발생시킨다. 커패시터는, 제2 영역에서 투명 기판의 제2 표면 위에 광 박막 트랜지스터와 나란하게 배치되어 광 박막 트랜지스터로부터 발생된 전하를 저장한다. 그리고 전달 박막 트랜지스터는, 제3 영역에서 투명 기판의 제2 표면 위에 커패시터와 나란하게 배치되어 커패시터에 저장된 전하를 별도의 분석 시스템에 전달한다.
Abstract:
PURPOSE: To provide a micro-fluidic device capable of delaying transfer of fluid for a certain time by simple structure and principle. CONSTITUTION: In a micro-fluidic device using capillary phenomenon, the micro-fluidic device capable of controlling flow time of micro-fluid comprises a fluid transferring flow path (310) formed between an upper substrate and a lower substrate or between the upper substrate and the lower substrate and an intermediate substrate; a fluid stopping surface (325) for temporarily stopping a fluid transferred through the flow path; and a flow delay projection (345) formed on a continuous line extended from the fluid stopping surface.
Abstract:
PURPOSE: A low power consumption microfabricated thermal cycler and a method for fabrication of the same are provided, thereby accurately and minutely controlling the temperature of the reaction occurring area, so that it can be applied to various bio chips including PCR chip, protein chip, DNA chip, drug delivery system, micro biological/chemical reactor and lab-on-a-chip. CONSTITUTION: The low power consumption microfabricated thermal cycler comprises an upper board(112) and a lower board(100), wherein the upper board(112) comprises an fluid inlet(114) and outlet, a reaction chamber(118), and a fluid channel(116) connecting the inlet(114) and outlet to the reaction chamber(118); and the lower board(100) comprises an insulated heating thin layer(106) formed on the lower board(100), a heating means(102) formed on the insulated heating thin layer(106), a temperature sensor(104) formed on the insulated heating thin layer(106), and an insulating layer(108) covering the heating means(102) and the temperature sensor(104); and the insulated heating thin layer(106) is composed of Si3N4, SiO2, Si3N4/SiO2/Si3N4 or SiO2/Si3N4/SiO2 and has thickness of 0.1 to 10 micrometer.
Abstract:
PURPOSE: Provided is a surface tension-controlled microfluidic device which moves the fluid only through natural flowing due to the capillary attraction in a controlled manner, and is effectively used in bio chips requiring micro and precise flow control. CONSTITUTION: A surface tension-controlled microfluidic device comprises an upper substrate and a lower substrate(100) for controlling and reacting the fluid. The lower substrate comprises: first and second storage chambers(102,104) for storing first and second fluids, respectively; at least one sensor chamber(106) connected to the first and second storage chambers; first and second flow stoppers(112,114) for stopping the flows of the first and second fluids; a flow delayer(116) for reducing the flow speed of the first or second fluids; a waste chamber(120) for waste-disposing the reacted first or second fluid; and a fluid passage(118) for interconnecting the flow delayer and the waste chamber to flow the fluid. The upper substrate comprises at least one sensor for measuring the biochemical reaction in the sensor chamber. The first fluid moves to the sensor chamber due to the capillary attraction to make the primary biochemical reaction, and after a predetermined time passes by, the second fluid flows into the sensor chamber due to the capillary attraction to replace the first fluid while making the secondary biochemical reaction.
Abstract:
PURPOSE: A hybridization method through periodic heating and cooling and a polynucleotide detection apparatus using the same are provided, thereby reducing the time for hybridization and increasing the specificity of hybridization, so that the disorder of target polynucleotide can be rapidly and accurately detected. CONSTITUTION: A hybridization method through periodic heating and cooling comprises the steps of: supplying a probe with a target polynucleotide; and circulating a high temperature hybridization process, wherein the hybridization is carried out at higher temperature than the melting point of the probe and target polynucleotide hybrid, and a low temperature hybridization process wherein the hybridization is carried out at lower temperature than the melting point of the probe and target polynucleotide hybrid two or more times, wherein the high temperature is at least 20 deg. C as higher as the melting point; and the low temperature is at least 5 deg. C as lower as the melting point.
Abstract:
본 발명은 미세 유체의 이송 시간을 제어할 수 있는 미세 유체 소자에 관한 것으로, 모세관 현상을 이용한 미세 유체 소자에 있어서, 상부 기판과 하부 기판의 사이에 위치한 유체 이송용 유로; 상기 유로를 통하여 이송된 유체가 일시적으로 정지되는 유체정지면; 및 상기 유체 정지면과 연속선상에 있는 유동지연턱을 포함하는 것을 특징으로 하는 미세 유체의 이송 시간을 제어할 수 있는 미세 유체 소자를 제공한다. 본 발명에 따른 미세 유체 소자는 간편한 방법으로 미세 유체의 이송 시간을 제어할 수 있어 매우 유용하다.