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公开(公告)号：KR1020110069283A
公开(公告)日：2011-06-23
申请号：KR1020090125952
申请日：2009-12-17
Applicant: 한국해양연구원
Abstract: PURPOSE: A gene encoding a protein isolated from Thermococcus sp. NA1 is provided to be applied to textile, food, paper, and distillation industries and clinical and pharmaceutical fields. CONSTITUTION: A useful gene isolated from Thermococcus sp. NA1 is selected from sequence numbers 1-2031. A useful protein isolated from Thermococcus sp. NA1 is selected from sequence numbers 2032-4062. A recombinant vector contains the useful gene. A transformed cell is obtained by transforming host cells of prokaryote, fungi, plant cells, and animal cells using the recombinant vector. The host cell is E.coli. The useful protein is prepared by culturing the transformed cells.
Abstract translation: 目的：编码从Thermococcus sp。分离的蛋白质的基因。 NA1适用于纺织，食品，造纸，蒸馏工业以及临床和制药领域。 构成：从Thermococcus sp。分离的有用基因。 NA1从序列号1-2031中选择。 从Thermococcus sp。分离的有用蛋白质 NA1选自序列号2032-4062。 重组载体含有有用的基因。 通过使用重组载体转化原核生物，真菌，植物细胞和动物细胞的宿主细胞获得转化的细胞。 宿主细胞是大肠杆菌。 通过培养转化细胞制备有用的蛋白质。
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公开(公告)号：KR1020110046412A
公开(公告)日：2011-05-04
申请号：KR1020110025582
申请日：2011-03-22
Applicant: 한국해양연구원
Abstract: PURPOSE: A novel phosphatase isolated from Thermococcus strain and a gene encoding the same are provided to ensure thermal stability and to be used as a marker. CONSTITUTION: A novel phosphatase has an amino acid of sequence number 9. The novel phosphatase is produced from Thermococcus onnurineus NA1(deposit number: KCTC 10859BP) which generates hydrogen under anaerobic condition. A gene encoding the phosphatase contains a base sequence of sequence number 4. The novel phosphatase is produced by culturing Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：提供从Thermococcus菌株分离的新型磷酸酶及其编码基因，以确保热稳定性并用作标记物。 构成：新型磷酸酶具有序列号9的氨基酸。新型磷酸酶由嗜热厌氧条件下产生氢的Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）产生。 编码磷酸酶的基因含有序列号4的碱基序列。新型磷酸酶是通过培养嗜热热球菌NA1（保藏号：KCTC 10859BP）产生的。
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公开(公告)号：KR1020110046411A
公开(公告)日：2011-05-04
申请号：KR1020110025581
申请日：2011-03-22
Applicant: 한국해양연구원
Abstract: PURPOSE: A novel phosphatase isolated from Thermococcus onnurineus NA1 and agene encoding the same are provided to be used as a marker for detecting DNA object or certain protein. CONSTITUTION: A phosphatase isolated from Thermococcus onnurineus NA1 contains an amino acid of sequence number 8. The Thermococcus onnurineus NA1 has deposit number KCTC 10859BP. The novel strain is isolated from deep seawater in East Manus Basin. The novel phosphatase is produced by culturing Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：提供从Thermococcus Onnurineus NA1分离的新型磷酸酶和编码该蛋白的Agene，用作检测DNA物质或某些蛋白质的标记物。 构成：从Thermococcus onnurineus NA1分离的磷酸酶含有序列号8的氨基酸。Thermococcus onnurineus NA1具有保藏号KCTC 10859BP。 新型菌株从东马努斯盆地的深海水中分离出来。 新型磷酸酶通过培养嗜热热球菌NA1（保藏号：KCTC 10859BP）产生。
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公开(公告)号：KR100825279B1
公开(公告)日：2008-04-25
申请号：KR1020060119613
申请日：2006-11-30
Applicant: 한국해양연구원
IPC: C07K14/195
CPC classification number: C12N9/16
Abstract: A HAM-1 like protein is provided to increase the processivity of DNA polymerase when used with the DNA polymerase, thereby being used for all reactions requiring DNA polymerization and applied to DNA amplification requiring high sensitivity and rapidity. A protein is described as SEQ ID : NO. 1 and is characterized in that it increases DNA polymerization processivity. A nucleic acid sequence encodes the protein and is described as SEQ ID : NO. 2. The protein is used in order to increase DNA polymerization by DNA polymerase. A host cell transformed by a recombinant vector including a gene described as SEQ ID : NO. 2 is used in order to produce the protein. The DNA polymerase and the protein are used in order to increase DNA replication.
Abstract translation: 提供了一种类似HAM-1的蛋白质，以增加DNA聚合酶与DNA聚合酶的连续性，从而被用于需要DNA聚合的所有反应，并应用于需要高灵敏度和快速度的DNA扩增。 蛋白质描述为SEQ ID NO： 1，其特征在于其增加DNA聚合反应活性。 核酸序列编码该蛋白质并描述为SEQ ID NO： 2.蛋白质用于通过DNA聚合酶增加DNA聚合。 通过重组载体转化的宿主细胞，其包含如SEQ ID NO： 2用于产生蛋白质。 使用DNA聚合酶和蛋白质以增加DNA复制。
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公开(公告)号：KR1020080030889A
公开(公告)日：2008-04-07
申请号：KR1020060097450
申请日：2006-10-02
Applicant: 한국해양연구원
CPC classification number: C12N9/1252
Abstract: Mutant DNA polymerases are provided to change both of exonuclease activity and inosine sensing function in normal DNA polymerases isolated from Thermococcus sp., so that the mutant DNA polymerases are useful in polymerase chain reaction(PCR) using primers containing inosine. The mutant DNA polymerase contains 91st-106th amino acids and 205th-220th amino acids in the amino acid sequence of SEQ ID NO:1; 91st-106th amino acids, 133rd-148th amino acids, 205th-220th amino acids and 300th-315th amino acids in the amino acid sequence of SEQ ID NO:2; or 91st-106th amino acids, 107th-122nd amino acids, 133rd-148th amino acids, 205th-220th amino acids and 300th-315th amino acids in the amino acid sequence of SEQ ID NO:3. A gene encodes the mutant DNA polymerase having the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3. A recombinant vector contains the gene encoding the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3. A transformed host cell is produced by the recombinant vector.
Abstract translation: 提供突变DNA聚合酶以改变从Thermococcus sp。分离的正常DNA聚合酶中的核酸外切酶活性和肌苷感应功能，使得突变型DNA聚合酶可用于使用含有肌苷的引物的聚合酶链反应（PCR）。 突变型DNA聚合酶在SEQ ID NO：1的氨基酸序列中含有91st-106th氨基酸和205-22个氨基酸; SEQ ID NO：2的氨基酸序列中的第91位至第106位氨基酸，第133位-148位氨基酸，第205-第220位氨基酸和第300-第315位氨基酸; 或SEQ ID NO：3的氨基酸序列中的第91个至第106个氨基酸，第107-122个氨基酸，133-148个氨基酸，第205个至第220个氨基酸和第300-315个氨基酸。 A基因编码具有SEQ ID NO：1，SEQ ID NO：2或SEQ ID NO：3的氨基酸序列的突变型DNA聚合酶。 重组载体含有编码SEQ ID NO：1，SEQ ID NO：2或SEQ ID NO：3的氨基酸序列的基因。 通过重组载体产生转化的宿主细胞。
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Patent Agency Ranking
公开(公告)号：KR100777230B1
公开(公告)日：2007-11-28
申请号：KR1020060119612
申请日：2006-11-30
Applicant: 한국해양연구원
IPC: C12N9/12
CPC classification number: C12N9/1252
Abstract: A mutant DNA polymerase is provided to increase the processivity by inducing mutation at an exonuclease active portion compared to a conventional wild-type DNA polymerase, thereby being widely applied to various molecular genetics in PCR. A DNA polymerase includes at least one amino acid sequence selected from the group consisting of amino acid sequences consisting of 3rd to 9th sequences of SEQ ID : NO. 1, SEQ ID : NO. 2, SEQ ID : NO. 4, SEQ ID : NO. 6, SEQ ID : NO. 7, SEQ ID : NO. 8, SEQ ID : NO. 9, SEQ ID : NO. 10, SEQ ID : NO. 11, and SEQ ID : NO. 12. A nucleic acid sequence encodes at least one amino acid sequence selected from the group consisting of SEQ ID : NOs. 13 to 24. A method for preparing the polymerase comprises a step of culturing the host cell and inducing the expression of a recombinant protein to isolate the polymerase protein.
Abstract translation: 提供突变型DNA聚合酶以通过在外切核酸酶活性部分诱导与常规野生型DNA聚合酶相比的突变来增加活性，从而广泛应用于PCR中的各种分子遗传学。 DNA聚合酶包括至少一个选自由SEQ ID NO：3的第3至第9个序列组成的组的氨基酸序列的氨基酸序列。 1，SEQ ID NO： 2，SEQ ID：NO。 4，SEQ ID NO： 6，SEQ ID NO： 7，SEQ ID NO： 8，SEQ ID NO： 9，SEQ ID NO： 10，SEQ ID NO： 11和SEQ ID NO： 核酸序列编码至少一个选自SEQ ID NO：1的氨基酸序列。 一种制备聚合酶的方法包括培养宿主细胞并诱导重组蛋白的表达以分离聚合酶蛋白的步骤。
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公开(公告)号：KR100777228B1
公开(公告)日：2007-11-28
申请号：KR1020060033297
申请日：2006-04-12
Applicant: 한국해양연구원
Abstract: 본 발명은 고호열성 디유티피아제(dUTPase) 및 이의 제조방법에 관한 것으로서,
Thermococcus sp. 균주로부터 분리되어진 신규의 고호열성 dUTPase 및 이의 기능적 동등물, 이들의 아미노산 서열을 가진 단백질 및 고호열성 dUTPase 생산방법을 제공한다. 본 발명에 따른 dUTPase는 고호열성이며, PCR 반응시 첨가가능하며, PCR 반응액에 존재하는 dUTP로 인한 높은 충실도 DNA 중합효소의 정지현상을 줄여서, 정밀한 PCR이 요구되는 정밀분석, 정밀진단. 동정 등에 매우 유용하게 사용될 수 있다.
디유티피아제, 고호열성-
公开(公告)号：KR100770665B1
公开(公告)日：2007-10-29
申请号：KR1020050103487
申请日：2005-10-31
Applicant: 한국해양연구원
Abstract: 본 발명은 고호열성 프로릴올리고펩티다아제 효소 및 이의 제조방법에 관한 것으로서,
Thermococcus sp. 균주(KCTC 10859BP)로부터 분리되어진 신규의 고호열성 프로릴올리고펩티다아제 및 이의 기능적 동등물, 이들을 암호화하는 신규의 유전자 및 이들의 생산방법을 제공한다. 본 발명에 따른 단백질 분해 효소는 고호열성이다.
단백질분해효소, 프로릴올리고펩티다아제, 고호열성-
公开(公告)号：KR1020070101892A
公开(公告)日：2007-10-18
申请号：KR1020060033297
申请日：2006-04-12
Applicant: 한국해양연구원
Abstract: An enzyme hyperthermophilic dUTPase is provided to increase fidelity of DNA polymerase by converting dUTP(deoxyuridine triphosphate) into dUMP(deoxyuridine monophosphate) and biphosphate in PCR(polymerase chain reaction) reaction solution, thereby reducing dUTP level in the solution, and stably maintain its activity at high temperature, so that it is useful in PCR reaction. A hyperthermophilic dUTPase isolated from Thermococcus sp. NA1 has the amino acid sequence of SEQ ID NO:2 and is encoded by a gene having the nucleotide sequence of SEQ ID NO:1. A method for preparing the hyperthermophilic dUTPase comprises the steps of: constructing a recombinant vector containing a gene encoding the hyperthermophilic dUTPase; transforming a host cell with the recombinant vector; and culturing the transformed cell and separating the hyperthermophilic dUTPase from the cultured cell.
Abstract translation: 通过在PCR（聚合酶链反应）反应溶液中将dUTP（脱氧尿苷三磷酸）转化为dUMP（脱氧尿苷单磷酸酯）和二磷酸酯，从而降低溶液中的dUTP水平，并稳定维持其活性，提供酶超嗜热dUTP酶以提高DNA聚合酶的保真度 在高温下使其在PCR反应中有用。 从Thermococcus sp。分离的超嗜热dUTPase NA1具有SEQ ID NO：2的氨基酸序列，并由具有SEQ ID NO：1的核苷酸序列的基因编码。 制备超嗜热性dUTP酶的方法包括以下步骤：构建含有编码超嗜热性dUTP酶的基因的重组载体; 用重组载体转化宿主细胞; 培养转化的细胞并从培养的细胞中分离超嗜热dUTP酶。
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