公开(公告)号：KR100825279B1

公开(公告)日：2008-04-25

申请号：KR1020060119613

申请日：2006-11-30

Applicant: 한국해양연구원

Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  유용구 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  정인순 ,  권석태

IPC: C07K14/195

CPC classification number: C12N9/16

Abstract: A HAM-1 like protein is provided to increase the processivity of DNA polymerase when used with the DNA polymerase, thereby being used for all reactions requiring DNA polymerization and applied to DNA amplification requiring high sensitivity and rapidity. A protein is described as SEQ ID : NO. 1 and is characterized in that it increases DNA polymerization processivity. A nucleic acid sequence encodes the protein and is described as SEQ ID : NO. 2. The protein is used in order to increase DNA polymerization by DNA polymerase. A host cell transformed by a recombinant vector including a gene described as SEQ ID : NO. 2 is used in order to produce the protein. The DNA polymerase and the protein are used in order to increase DNA replication.

Abstract translation: 提供了一种类似HAM-1的蛋白质，以增加DNA聚合酶与DNA聚合酶的连续性，从而被用于需要DNA聚合的所有反应，并应用于需要高灵敏度和快速度的DNA扩增。 蛋白质描述为SEQ ID NO： 1，其特征在于其增加DNA聚合反应活性。 核酸序列编码该蛋白质并描述为SEQ ID NO： 2.蛋白质用于通过DNA聚合酶增加DNA聚合。 通过重组载体转化的宿主细胞，其包含如SEQ ID NO： 2用于产生蛋白质。 使用DNA聚合酶和蛋白质以增加DNA复制。

公开(公告)号：KR100777230B1

公开(公告)日：2007-11-28

申请号：KR1020060119612

申请日：2006-11-30

Applicant: 한국해양연구원

Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  유용구 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  정인순 ,  권석태 ,  차선신

IPC: C12N9/12

CPC classification number: C12N9/1252

Abstract: A mutant DNA polymerase is provided to increase the processivity by inducing mutation at an exonuclease active portion compared to a conventional wild-type DNA polymerase, thereby being widely applied to various molecular genetics in PCR. A DNA polymerase includes at least one amino acid sequence selected from the group consisting of amino acid sequences consisting of 3rd to 9th sequences of SEQ ID : NO. 1, SEQ ID : NO. 2, SEQ ID : NO. 4, SEQ ID : NO. 6, SEQ ID : NO. 7, SEQ ID : NO. 8, SEQ ID : NO. 9, SEQ ID : NO. 10, SEQ ID : NO. 11, and SEQ ID : NO. 12. A nucleic acid sequence encodes at least one amino acid sequence selected from the group consisting of SEQ ID : NOs. 13 to 24. A method for preparing the polymerase comprises a step of culturing the host cell and inducing the expression of a recombinant protein to isolate the polymerase protein.

Abstract translation: 提供突变型DNA聚合酶以通过在外切核酸酶活性部分诱导与常规野生型DNA聚合酶相比的突变来增加活性，从而广泛应用于PCR中的各种分子遗传学。 DNA聚合酶包括至少一个选自由SEQ ID NO：3的第3至第9个序列组成的组的氨基酸序列的氨基酸序列。 1，SEQ ID NO： 2，SEQ ID：NO。 4，SEQ ID NO： 6，SEQ ID NO： 7，SEQ ID NO： 8，SEQ ID NO： 9，SEQ ID NO： 10，SEQ ID NO： 11和SEQ ID NO： 核酸序列编码至少一个选自SEQ ID NO：1的氨基酸序列。 一种制备聚合酶的方法包括培养宿主细胞并诱导重组蛋白的表达以分离聚合酶蛋白的步骤。