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公开(公告)号：WO2010027233A3
公开(公告)日：2010-03-11
申请号：PCT/KR2009/005060
申请日：2009-09-07
Abstract: 본 발명은 Thermococcus 속에 속하는 고호열성 신균주로부터 분리한 신규한 수소화효소(hydrogenase), 이를 암호화하는 유전자 및 이들을 이용하여 수소를 생 산하는 방법에 관한 것이다. 본 발명의 수소생산방법에 따르면 상기 균주를 특정 배양조건으로 배양하는 것만으로 많은 양의 수소를 생산할 수 있으므로, 기존의 수 소생산방법에 비하여 경제적이고 효율적이며, 고온에서도 수소를 생산할 수 있는 장점이 있다.
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2.发明公开Ｆｕｓｏｂａｃｔｅｒｉａ ｓｐ. ＰＮＢ1으로부터의 신규한저온성 단백질분해효소 및 이를 암호화하는 유전자 无效来自FUSOBACTERIA SP的新型PSYCHROPHILIC PROTEASES PNB1及其基因
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公开(公告)号：KR1020090060147A
公开(公告)日：2009-06-11
申请号：KR1020080116102
申请日：2008-11-21
Applicant: 한국해양연구원
Abstract: Five phosphatases are provided to efficiently detect a specific protein or DAN object. A phosphatase is produced by hyperthermophilic strain, thermococcus onnurineus NA1 (KCTC 10859BP). The phosphatase comprises an amino acide of the sequences 6-10. A gene coding the phosphatase comprises a base of sequences 1-5.
Abstract translation: 提供五种磷酸酶以有效检测特定蛋白质或DAN对象。 磷酸酶由超嗜热菌株，热球菌NA1（KCTC 10859BP）产生。 磷酸酶包含序列6-10的氨基酸。 编码磷酸酶的基因包含序列1-5的碱基。
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Patent Agency Ranking
公开(公告)号：KR101203920B1
公开(公告)日：2012-11-22
申请号：KR1020080116102
申请日：2008-11-21
Applicant: 한국해양연구원
Abstract: 본 발명은
Thermococcus 속에 속하는 고호열성 신균주로부터 분리한 신규한 포스파타제(Phosphatase) 및 이를 암호화하는 유전자에 관한 것이다. 본 발명의 포스파타제는 고온에서도 열안정성을 갖기 때문에 특정 단백질이나 DNA 대상체를 검출하기 위한 마커 등으로 널리 이용될 수 있다.
포스파타제, HAD 슈퍼패밀리-
公开(公告)号：KR1020110069283A
公开(公告)日：2011-06-23
申请号：KR1020090125952
申请日：2009-12-17
Applicant: 한국해양연구원
Abstract: PURPOSE: A gene encoding a protein isolated from Thermococcus sp. NA1 is provided to be applied to textile, food, paper, and distillation industries and clinical and pharmaceutical fields. CONSTITUTION: A useful gene isolated from Thermococcus sp. NA1 is selected from sequence numbers 1-2031. A useful protein isolated from Thermococcus sp. NA1 is selected from sequence numbers 2032-4062. A recombinant vector contains the useful gene. A transformed cell is obtained by transforming host cells of prokaryote, fungi, plant cells, and animal cells using the recombinant vector. The host cell is E.coli. The useful protein is prepared by culturing the transformed cells.
Abstract translation: 目的：编码从Thermococcus sp。分离的蛋白质的基因。 NA1适用于纺织，食品，造纸，蒸馏工业以及临床和制药领域。 构成：从Thermococcus sp。分离的有用基因。 NA1从序列号1-2031中选择。 从Thermococcus sp。分离的有用蛋白质 NA1选自序列号2032-4062。 重组载体含有有用的基因。 通过使用重组载体转化原核生物，真菌，植物细胞和动物细胞的宿主细胞获得转化的细胞。 宿主细胞是大肠杆菌。 通过培养转化细胞制备有用的蛋白质。
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公开(公告)号：KR100825279B1
公开(公告)日：2008-04-25
申请号：KR1020060119613
申请日：2006-11-30
Applicant: 한국해양연구원
IPC: C07K14/195
CPC classification number: C12N9/16
Abstract: A HAM-1 like protein is provided to increase the processivity of DNA polymerase when used with the DNA polymerase, thereby being used for all reactions requiring DNA polymerization and applied to DNA amplification requiring high sensitivity and rapidity. A protein is described as SEQ ID : NO. 1 and is characterized in that it increases DNA polymerization processivity. A nucleic acid sequence encodes the protein and is described as SEQ ID : NO. 2. The protein is used in order to increase DNA polymerization by DNA polymerase. A host cell transformed by a recombinant vector including a gene described as SEQ ID : NO. 2 is used in order to produce the protein. The DNA polymerase and the protein are used in order to increase DNA replication.
Abstract translation: 提供了一种类似HAM-1的蛋白质，以增加DNA聚合酶与DNA聚合酶的连续性，从而被用于需要DNA聚合的所有反应，并应用于需要高灵敏度和快速度的DNA扩增。 蛋白质描述为SEQ ID NO： 1，其特征在于其增加DNA聚合反应活性。 核酸序列编码该蛋白质并描述为SEQ ID NO： 2.蛋白质用于通过DNA聚合酶增加DNA聚合。 通过重组载体转化的宿主细胞，其包含如SEQ ID NO： 2用于产生蛋白质。 使用DNA聚合酶和蛋白质以增加DNA复制。
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公开(公告)号：KR100777230B1
公开(公告)日：2007-11-28
申请号：KR1020060119612
申请日：2006-11-30
Applicant: 한국해양연구원
IPC: C12N9/12
CPC classification number: C12N9/1252
Abstract: A mutant DNA polymerase is provided to increase the processivity by inducing mutation at an exonuclease active portion compared to a conventional wild-type DNA polymerase, thereby being widely applied to various molecular genetics in PCR. A DNA polymerase includes at least one amino acid sequence selected from the group consisting of amino acid sequences consisting of 3rd to 9th sequences of SEQ ID : NO. 1, SEQ ID : NO. 2, SEQ ID : NO. 4, SEQ ID : NO. 6, SEQ ID : NO. 7, SEQ ID : NO. 8, SEQ ID : NO. 9, SEQ ID : NO. 10, SEQ ID : NO. 11, and SEQ ID : NO. 12. A nucleic acid sequence encodes at least one amino acid sequence selected from the group consisting of SEQ ID : NOs. 13 to 24. A method for preparing the polymerase comprises a step of culturing the host cell and inducing the expression of a recombinant protein to isolate the polymerase protein.
Abstract translation: 提供突变型DNA聚合酶以通过在外切核酸酶活性部分诱导与常规野生型DNA聚合酶相比的突变来增加活性，从而广泛应用于PCR中的各种分子遗传学。 DNA聚合酶包括至少一个选自由SEQ ID NO：3的第3至第9个序列组成的组的氨基酸序列的氨基酸序列。 1，SEQ ID NO： 2，SEQ ID：NO。 4，SEQ ID NO： 6，SEQ ID NO： 7，SEQ ID NO： 8，SEQ ID NO： 9，SEQ ID NO： 10，SEQ ID NO： 11和SEQ ID NO： 核酸序列编码至少一个选自SEQ ID NO：1的氨基酸序列。 一种制备聚合酶的方法包括培养宿主细胞并诱导重组蛋白的表达以分离聚合酶蛋白的步骤。
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8.发明公开Ｔｈｅｒｍｏｃｏｃｃｕｓ ｓｐｐ.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效从热精球菌中纯化的新型氢化物，编码基因的方法以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020090060124A
公开(公告)日：2009-06-11
申请号：KR1020080087806
申请日：2008-09-05
Applicant: 한국해양연구원
Abstract: A hydrogenase which is isolated from a Thermococcus spp. is provided to economically and efficiently produce hydrogen by culturing a hyperthermophilic strain under the specific culture condition. A hydrogenase comprises an amino acid selected from the sequence numbers 1-112. A gene coding the hydrogenase comprises base of the sequence numbers 113-225. A method for producing a hydrogen comprises culturing Thermococcus spp. The Thermococcus spp. is Thermococcus onnurineus NA1(KCTC 10859BP). The culture is culturing in a medium of anaerobic condition supplying carbon monoxide.
Abstract translation: 从热球菌属中分离的氢化酶。 通过在特定培养条件下培养超嗜热菌株来经济地和有效地产生氢。 氢化酶包含选自序列号1-112的氨基酸。 编码氢化酶的基因包含序列号113-225的碱基。 一种生产氢的方法包括培养Thermococcus spp。 热球菌属 是Thermococcus onnurineus NA1（KCTC 10859BP）。 培养物在一氧化碳的厌氧条件培养基中培养。
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