Abstract:
A porous biodegradable polymer transplant for the semilunar cartilage substitution is provided to allow semilunar cartilage to be regenerated effectively and to protect joint cartilage. A porous biodegradable polymer transplant for the semilunar cartilage substitution comprises a porous biodegradable polymer support of semilunar cartilage shape; and the homo-synovium cell disseminated in the support. The porous biodegradable polymer support is polycaprolactone. The porous biodegradable polymer support has a pore size of 100-150 micrometers, a porosity of 80-95%, a compressive strength of 380-460 kPa and a seam strength of 2.0-2.8 kg.
Abstract:
A method for manufacturing a membrane type bioactive ceramic non-woven fabric is provided to allow generating low crystalline apatite which has large specific surface area and good hydrophilic property and is similar to the bone in human body. A method for manufacturing a membrane type bioactive ceramic non-woven fabric comprises: preparing the first reactant by adding diluted alcohol 1 to metal alkoxide(S1); preparing the second reactant by adding water, calcium salt, and acid catalyzer, wherein the water is added in a mol ratio of 1-3(S2); hydrating the metal alkoxide by adding the reactant 2 to the reactant 1(S3); preparing ceramic polymer by condensing the hydrated metal alkoxide through heat treatment and dehydration(S4); and producing non-woven fabric by electro-spinning the prepared ceramic polymer(S5).
Abstract:
본 발명은 표면에 골조직 형성 증진 펩타이드가 고정된 골이식재 및 조직공학용 지지체에 관한 것으로, 보다 구체적으로 세포부착 유도 펩타이드 및/또는 조직성장인자 유래 펩타이드가 표면에 고정된 골이식재 및 조직공학용 지지체에 관한 것이다. 본 발명의 골이식재 및 조직공학용 지지체는 표면에 부착된 골조직 형성 증진 펩타이드에 의해 재생에 관련된 세포의 이행, 증식 및 분화를 촉진하여 최종적으로 조직재생력을 극대화시킬 수 있다. 또한 표면에 고정된 펩타이드는 분자량이 적어 체내에 적용시 면역반응의 위험이 적고, 체내에서 안정한 형태로 존재할 수 있어 약효의 지속화가 가능하여, 치주조직, 치조골재생 및 기타 골조직 재생 수술시 용이하여 수술 편의성이 우수하며 또한 높은 치료효과를 기대할 수 있다. 조직성장인자, 생리활성 펩타이드, 고정화, 골이식재, 지지체, 골재생력
Abstract:
PURPOSE: An olfactory receptor-functionalized transistors for a highly selective bioelectronic nose and a biosensor using the same are provided to implement remarkably improved selectivity and sensitivity by using a nanostructure transistor as an odorant receptor. CONSTITUTION: In a olfactory receptor-functionalized transistors for a highly selective bioelectronic nose and a biosensor using the same, a source electrode and a drain electrode are separated from each other on a substrate. A nanostructure electrically is contacted with the source electrode and drain electrode, and a thin film having an olfactory receptor protein covers the surface of the source electrode and the drain electrode and nanostructure surface. The olfactory receptor protein includes a cysteine residue, and the olfactory receptor protein is changed from a neutral acid form to a negatively-charged base form when being combined with a specific odor material(17).
Abstract:
A method for identifying synovium cell which proliferative or chondrogenic potential is decreased is provided to easily and accurately identify the synovium cell. A kit for identifying or detecting a synovium which proliferative or chondrogenic potential is decreased comprises: an antibody which specifically binds to CD166, CD49a or CD106; and a probe or primer which specifically binds to the sequence of cyclin-dependent kinase suppressor 2A gene, v-Ki-ras 2 gene, thrombospondin 1 gene, mitogen activated protein kinase 9 gene, tumor necrosis factor receptor super family member 6 gene, tumor necrosis factor receptor super family member 10d gene or PRKC apoptosis WT1 regulator (PAWR) gene. A method for identifying or detecting the synovium cell comprises: a step of containing an antibody which specifically binds to a test cell to analyze in CD166, CD49a or CD106; and a step of determining the bind of the antibody on the surface of the cell.
Abstract:
A manufacturing method of a bone grafting substitute using a horse bone is provided to remove effectively fat and organic matters from horse bone that has a structure similar to that of a human. A manufacturing method of a bone grafting substitute using a horse bone includes the steps of: boiling horse bone to remove fat and protein and drying the horse bone; pulverizing the dried bone and precipitating the bone powder in organic solvent; removing the organic solvent and drying; making the bone powder treated with sodium hypochlorite solution having a density of 2-20%; removing the sodium hypochlorite solution and impurities and drying the bone powder; adding purified water to the bone powder, performing compression and hydrothermal treatment at 120-124deg.C for 30 minutes to 5 hours and drying the bone powder; and performing thermal treatment of bone powder at 500-1,000deg.C for 1-24hours.
Abstract:
본 발명은 표면에 골조직 형성 증진 펩타이드가 고정된 골이식재 및 조직공학용 지지체에 관한 것으로, 보다 구체적으로 세포부착 유도 펩타이드 및/또는 조직성장인자 유래 펩타이드가 표면에 고정된 골이식재 및 조직공학용 지지체에 관한 것이다. 본 발명의 골이식재 및 조직공학용 지지체는 표면에 부착된 골조직 형성 증진 펩타이드에 의해 재생에 관련된 세포의 이행, 증식 및 분화를 촉진하여 최종적으로 조직재생력을 극대화시킬 수 있다. 또한 표면에 고정된 펩타이드는 분자량이 적어 체내에 적용시 면역반응의 위험이 적고, 체내에서 안정한 형태로 존재할 수 있어 약효의 지속화가 가능하여, 치주조직, 치조골재생 및 기타 골조직 재생 수술시 용이하여 수술 편의성이 우수하며 또한 높은 치료효과를 기대할 수 있다. 조직성장인자, 생리활성 펩타이드, 고정화, 골이식재, 지지체, 골재생력
Abstract:
본 발명은 (a) CD166, CD49a 또는 CD106에 특이적으로 결합하는 항체; 또는 (b) 사이클린-의존성 키나아제 억제자 2A 유전자, v-Ki-ras 2 유전자, 트롬보스폰딘 1 유전자, 마이토젠-활성 단백질 키나아제 9 유전자, 엑토뉴클레오타이드 파이로포스파타아제/포스포다이에스터라아제 1 유전자, 종양괴사인자 수용체 슈퍼패밀리 멤머 6 유전자, 종양괴사인자 수용체 슈퍼패밀리 멤버 10d 유전자, 종양괴사인자 수용체 슈퍼패밀리 멤머 10b 유전자, 종양괴사인자 수용체 슈퍼패밀리 11b 유전자, TNF 수용체-연관 인자 4 유전자 또는 PRKC 아폽토시스 WT1 조절자(PAWR) 유전자 서열에 특이적으로 결합하는 프라이머 또는 프로브를 포함하는 증식능 또는 연골생성능이 감소된 활액막 세포의 동정 또는 검출용 키트를 제공한다. 본 발명에 따르면, 증식능 및/또는 연골생성능이 감소된 활액막 세포를 용이하면서도 정확하게 동정할 수 있으며, 본 발명은 활액막 세포를 이용한 관절연골의 손상 치료에 새로운 단서를 제공한다.
Abstract:
PURPOSE: A method for identifying synovial cells with reduced proliferation ability or chondrogenecity is provided to accurately and easily identify synobial cells. CONSTITUTION: A kit for identifying or detecting synovial cells with reduced proliferation ability or chondrogenecity contains a primer or probe which specifically binds to PRKC apoptosis WT1 regulator(PAWR) gene sequence. A method for identifying or detecting synovial cells comprises: a step of isolating total RNA from synobial cells; a step of synthesizing cDNA from the total RNA; a step of hybridizing the cDNA to the probe; and a step of confirming hybridization.
Abstract:
PURPOSE: A method for identifying synovial cells with reduced proliferation ability or chondrogenecity is provided to easily and accurately identify synovial cells. CONSTITUTION: A kit for identifying or detecting synovial cells with proliferation ability or chondrogenecity contains a primer or probe which specifically binds to TNF receptor super family 11n gene sequence. A method for identifying or detecting synovial cells with reduce proliferation ability or chondrogenecity comprises: a step of preparing total RNA from synovial cells; a step of synthesizing cDNA from the total RNA; a step of hybridizing the synovial cell-derived cDNA to the probe; and a step of confirming hybridization.