公开(公告)号：FR2860872A1

公开(公告)日：2005-04-15

申请号：FR0350663

申请日：2003-10-09

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  BIJEON JEAN LOUIS ,  ROYER PASCAL ,  ADAM PIERRE MICHEL

IPC: C12Q1/68 ,  G01N21/55 ,  G01N21/64 ,  G01N21/65 ,  G01N13/14 ,  G01N33/53 ,  B81B7/04

Abstract: A surface plasmon microsensor or a nanosensor for chemical or biological species including pads distributed on the surface of a support, the pads including an electrically conductive material and being capable of immobilizing the chemical or biological species. The pads have a dimension less than 1 μm.

公开(公告)号：FR2859781A1

公开(公告)日：2005-03-18

申请号：FR0350560

申请日：2003-09-17

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： PETIT JEROME ,  CHATON PATRICK ,  GRAND GILLES ,  LEROUX THIERRY

IPC: G01B11/06 ,  G01B11/24 ,  G01B11/30 ,  G01N21/956 ,  G01B11/00

Abstract: The device has a measuring objective (2) to form an image from an optical Fourier transform of an elementary surface of an object in a Fourier image focal plane. A transfer unit (10, 12) forms the image on a CCD camera (14). A calculating unit (26) produces data concerning dimensional and/or structural characteristics of the object or identifies the characteristics from information provided by the camera. An independent claim is also included for a method to measure dimensional and/or structural characteristics of an object.

公开(公告)号：FR2854696A1

公开(公告)日：2004-11-12

申请号：FR0350146

申请日：2003-05-06

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  GETIN STEPHANE ,  VINET FRANCOISE ,  PEGON PHILIPPE ,  GERMAIN CHANTAL

IPC: C03C17/00 ,  C03C17/42 ,  G01N33/543 ,  G01N21/64

Abstract: The invention relates to a biochip support comprising a substrate supporting at least one porous layer of material on a first face, the said layer being designed to fix biological molecules onto the said layer and in the volume of this layer, the said support being characterized in that the said layer is a thin optical layer of material prepared by the sol-gel method and for which the refraction index is less than the refraction index of the substrate. The invention also relates to a process grafting of biological molecules onto and into the thin layer of material prepared by the sol-gel method on the first face of the biochip support. This process comprises the following steps: a sol is prepared that will provide the sol-gel material, biomolecules are incorporated into the material during its preparation, biomolecules are grafted into the material during its preparation, a thin layer of the said sol is deposited on the first face of the substrate, the thin layer of sol-gel material is obtained starting from the thin layer of sol. Finally, one particular embodiment of the grafting process also comprises a structuring step of the thin layer of sol-gel material to obtain a network of pads or wells over all or part of the biochip support.

公开(公告)号：FR2818382A1

公开(公告)日：2002-06-21

申请号：FR0016650

申请日：2000-12-20

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  VINET FRANCOISE

IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  G01N33/543 ,  G01N37/00 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/68

Abstract: The invention concerns an analysis support for determining an analyte such as a target DNA or RNA, by using a specific binding reaction (hybridization) of said analyte with a ligand specific of said analyte, and determining the binding reaction using at least two fluorescent markers present on the analyte. Said determination is carried out by applying an excitation field and by detecting the fluorescent emission of the different fluorescent markers. The invention is characterised in that the support comprises a substrate coated with one or several material layers forming an assembly capable of attenuating or increasing the excitation field for at least one of the fluorescent markers relative to the others. The ligands are fixed on the last layer of the assembly. A SiO2 layer on a silicon substrate can be used with the Cy3 and Cy5 fluorescent markers.

公开(公告)号：FR2818378A1

公开(公告)日：2002-06-21

申请号：FR0016317

申请日：2000-12-14

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  VINET FRANCOISE ,  BARRITAULT PIERRE ,  GETIN STEPHANE

IPC: G01N21/64 ,  G01N33/53 ,  G01N37/00 ,  G01N33/533 ,  G01N33/543 ,  G01N33/68 ,  H01L49/02

Abstract: The invention concerns a device for enhancing fluorescence comprising a support (10) supporting fluorescence enhancing means (11), the fluorescence enhancing means providing a surface for receiving chemical or biological elements to be read by detection of a fluorescence signal emitted by a fluorophore, associated with the chemical or biological elements, under the effect of an excitation light beam. The fluorescence enhancing means (11) consist of a thin transparent dielectric layer or a stack of thin transparent dielectric layers (12 to 16) ensuring a mirror function for the fluorescence signals and for the excitation light beam, the material of the thin layer or of each thin layer of the stack being selected among the following materials: TiO2, Ta2O5, HfO2, ZrO2, MgO, SiO2, Si3N4, MgF2, YF3. The invention is applicable to a biological or chemical optical sensor.

公开(公告)号：DE60138302D1

公开(公告)日：2009-05-20

申请号：DE60138302

申请日：2001-12-19

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  VINET FRANCOISE

IPC: G01N33/53 ,  G01N33/543 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/64 ,  G01N37/00

Abstract: The invention relates to an analytical support for determining an analyte such as a DNA or RNA target, by carrying out a specific reaction of binding (hybridization) of said analyte with a ligand specific for this analyte, and determining the binding reaction by means of at least two fluorescent labels present on the analyte. This determination is carried out by applying an excitatory field and detecting the fluorescent emission of the various fluorescent labels. According to the invention, the support comprises a substrate coated with one or more layers of material(s) forming an assembly capable of decreasing or increasing the excitatory field for at least one of the fluorescent labels compared to the others. The ligands are attached to the final layer of the assembly. A layer of SiO 2 on a substrate of silicon can be used with the fluorescent labels Cy3 and Cy5.

公开(公告)号：FR2864622A1

公开(公告)日：2005-07-01

申请号：FR0351212

申请日：2003-12-26

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： TREBBIA JEAN BAPTISTE ,  CHATON PATRICK ,  GETIN STEPHANE ,  GROSSE PHILIPPE

IPC: G01N33/483 ,  G01N21/17 ,  G01N21/25 ,  G01N21/39 ,  G01N21/59 ,  G01N21/27 ,  G01N33/543 ,  G01N33/68

Abstract: The measuring apparatus contains first (207) and second (209) light reflectors which have at least two mirrors or a matrix of mirrors (208) integrated in a single support, and the first and second reflectors form a system of resonant cavities. A support (200) such as a biochip with a number of biological samples (204) is placed between the first and second reflectors. At least one light pulse with a time span of between 100 picoseconds and 100 nanoseconds is emitted in the resonant cavities, and the responding light signals from the cavities are measured with the aid of one or more photodiodes, enabling the light absorption of the samples, containing DNA or RNA marked by fluorophores, to be determined.

公开(公告)号：FR2818263B1

公开(公告)日：2004-02-20

申请号：FR0016315

申请日：2000-12-14

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： POUTEAU PATRICK ,  CHATON PATRICK ,  PERRAUT FRANCOIS

IPC: G03F7/004 ,  G02B1/10 ,  G02B5/26 ,  G03F7/09 ,  G03F7/20 ,  B81C1/00 ,  G02B27/02 ,  G02B21/34 ,  C12Q1/68 ,  G02B21/26

Abstract: This invention relates to a substrate (1) with a reception surface (4) for a layer of material to be insolated by insolation light, wherein means forming a mirror (3) are arranged between said reception surface (4) and the layer of material to be insolated, these means forming a mirror (3) operating for the wavelength of the insolation light. This type of substrate may be used for making microelectronic or microtechnological devices, or biochips.

公开(公告)号：FR2833716A1

公开(公告)日：2003-06-20

申请号：FR0116117

申请日：2001-12-13

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： GETIN STEPHANE ,  CHATON PATRICK

IPC: G01N21/64 ,  G01N15/00 ,  G21K1/00 ,  H01S3/00 ,  G02B1/10 ,  B01J19/08 ,  B07C5/344 ,  G01N21/01 ,  H05H3/04 ,  H05H15/00

Abstract: The particle displacement device has a silicon substrate (6) on which a thin strip (5) is deposited. A particle (P) that is to be displaced is placed on the strip. The strip has an optical thickness gradient along its longitudinal axis, such that the particle is moved along the longitudinal axis of the strip, when an electromagnetic wave (L) illuminates the device. Independent claims are also included for the following: (1) particle switching device; (2) particle sorting device; (3) particle analysis device; (4) optical particle displacement process; (5) particle switching process; (6) particle sorting process; and (7) particle analysis process.

公开(公告)号：FR2818378B1

公开(公告)日：2003-06-13

申请号：FR0016317

申请日：2000-12-14

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CHATON PATRICK ,  VINET FRANCOISE ,  BARRITAULT PIERRE ,  GETIN STEPHANE

IPC: G01N21/64 ,  G01N33/53 ,  G01N37/00 ,  G01N33/533 ,  G01N33/543 ,  G01N33/68 ,  H01L49/02

Abstract: The invention concerns a device for enhancing fluorescence comprising a support (10) carrying fluorescence enhancement means (11), the fluorescence enhancement means offering a reception surface for chemical or biological elements intended to be read by detection of a fluorescence signal emitted by a fluorophore, associated with the chemical or biological elements, under the effect of an excitation light beam. The fluorescence enhancement means (11) is made up of a thin, transparent, dielectric layer or a stack of thin, transparent, dielectric layers (12 to 16) ensuring a mirror function for the fluorescence signal and excitation light beam, the material of the thin layer or of each thin layer of the stack being chosen from among the following materials: TiO2, Ta2O5, HfO2, ZrO2, MgO, SiO2, Si3N4, MgF2 and YF3. The fluorescence enhancement device may be used for a biological or chemical optic sensor.