公开(公告)号：CA2807591A1

公开(公告)日：2004-10-21

申请号：CA2807591

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: C12M1/40 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/38 ,  C12P19/34 ,  C12Q1/68 ,  G01N1/00

公开(公告)号：CA2480728A1

公开(公告)日：2003-10-16

申请号：CA2480728

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN D ,  THRONDSET WILLIAM ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  LIAU YISH-HANN ,  JAVADI SHERVIN ,  DARIDON ANTOINE ,  CHOU HOU-PU

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

Abstract: The invention provides systems (2000), including microfluidic mechanisms, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. These mechanisms may enable controlle d input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any number of suitable times in the system to allow particles to be sorted, cultured, mixed, treate d, and/or assayed, among others. These combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：EP1499706A4

公开(公告)日：2010-11-03

申请号：EP03721509

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：EP1615721A4

公开(公告)日：2006-08-02

申请号：EP04758896

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/02 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10

CPC classification number: B01L3/00 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：CA2807564C

公开(公告)日：2014-01-28

申请号：CA2807564

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  C40B30/00 ,  C40B60/00 ,  G01N33/48

Abstract: A microfluidic device, with elastomeric components, and having a flow channel, wherein said flow channel has a plurality of blind flow channels opening into and in fluid communication with the flow channel, each blind flow channel having an aperture portion, a channel portion and a terminus portion, and wherein a region at the terminus of each blind flow channel defines a reaction site. Each blind flow channel is associated with a valve that when closed isolates the reaction site from the flow channel. The valves associated with each of the plurality of blind flow channels in fluid communication with the flow channel are under the control of a common control channel and are coordinately closed or opened.

公开(公告)号：CA2521171C

公开(公告)日：2013-05-28

申请号：CA2521171

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/02 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  B81B3/00 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  G01N1/10 ,  G01N33/48

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：CA2807564A1

公开(公告)日：2004-10-21

申请号：CA2807564

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  C40B30/00 ,  C40B60/00 ,  G01N33/48

公开(公告)号：CA2521171A1

公开(公告)日：2004-10-21

申请号：CA2521171

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： NASSEF HANY RAMEZ ,  UNGER MARC ,  FACER GEOFFREY ,  MCBRIDE LINCOLN ,  LUCERO MICHAEL

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10 ,  B01L11/00

Abstract: A variety of elastomeric-based microfluidic devices and methods for using an d manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagent s have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and ge ne expression analyses.

公开(公告)号：AU2004228678A1

公开(公告)日：2004-10-21

申请号：AU2004228678

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC ,  MCBRIDE LINCOLN ,  FACER GEOFFREY ,  LUCERO MICHAEL ,  NASSEF HANY RAMEZ

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.