公开(公告)号：DE3779704D1

公开(公告)日：1992-07-16

申请号：DE3779704

申请日：1987-08-01

Applicant: HOECHST AG

Inventor： SIMONS GUIDO DR ,  STRECKER HELMUT DR ,  MOLZ PETER DR ,  SCHNORR GERD DR ,  SKRZIPCZYK HEINZ JUERGEN DR ,  WISSMANN HANS DR

IPC: G01N33/53 ,  G01N33/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/577 ,  G01N33/60 ,  G01N33/74 ,  G01N33/78 ,  G01N33/82

Abstract: Determn. of the concn. of the free portion of an active ingredient (I) in a biological fluid in presence of its natural binder (II), the bound and free fractions of (I) being in equilibrium, comprises (1) treating the test sample with unlabelled antibody (Ab); (2) sepg. sample and Ab; (3) incubating Ab with a labelled, cross-reactive tracer (T), and (4) measuring amt. of T which is bound or unbound, and from this calculating the concn. of free (I). The novelty is that the amt. of Ab and/or its affinity to (I) is such that it does not significantly alter the equilibrium between free and bound forms, while T has a significantly higher or lower affinity for Ab than (I) itself. Also new is a test kit for the process. Specifically, Ab can be monoclonal or polyclonal, and T is labelled with a radioactive atom; fluoroescent or chemiluminescent gp.; enzyme or chromophore.

公开(公告)号：DE3633497A1

公开(公告)日：1988-04-14

申请号：DE3633497

申请日：1986-10-02

Applicant: HOECHST AG

Inventor： SCHNORR GERD DR ,  STRECKER HELMUT DR ,  MOLZ PETER DR ,  SIMONS GUIDO DR ,  SKRZIPCZYK HANS-JUERGEN DR

IPC: G01N33/541 ,  G01N33/543 ,  G01N33/574 ,  G01N33/577 ,  G01N33/76 ,  G01N33/78 ,  G01N33/557 ,  G01N33/58

公开(公告)号：GR3019470T3

公开(公告)日：1996-06-30

申请号：GR960400849

申请日：1996-03-29

Applicant: HOECHST AG

Inventor： MOLZ PETER DR ,  SKRZIPCZYK HEINZ-JUERGEN DR ,  LUEBBERS HENNING DR ,  STRECKER HELMUT DR ,  SCHNORR GERD DR ,  KINKEL TONIO DR

IPC: C07D219/04 ,  C07D401/12 ,  C09K11/06 ,  G01N33/532 ,  G01N33/533 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68

公开(公告)号：DE3770743D1

公开(公告)日：1991-07-18

申请号：DE3770743

申请日：1987-09-29

Applicant: HOECHST AG

Inventor： SCHNORR GERD DR ,  STRECKER HELMUT DR ,  MOLZ PETER DR ,  SIMONS GUIDO DR ,  SKRZIPCZYK HEINZ JUERGEN DR

IPC: G01N33/541 ,  G01N33/543 ,  G01N33/574 ,  G01N33/577 ,  G01N33/76 ,  G01N33/78

公开(公告)号：AT64467T

公开(公告)日：1991-06-15

申请号：AT87114166

申请日：1987-09-29

Applicant: HOECHST AG

Inventor： SCHNORR GERD DR ,  STRECKER HELMUT DR ,  MOLZ PETER DR ,  SIMONS GUIDO DR ,  SKRZIPCZYK HEINZ JUERGEN DR

IPC: G01N33/541 ,  G01N33/543 ,  G01N33/574 ,  G01N33/577 ,  G01N33/76 ,  G01N33/78

公开(公告)号：DE3919393A1

公开(公告)日：1990-12-20

申请号：DE3919393

申请日：1989-06-14

Applicant: HOECHST AG

Inventor： HACHMANN HENNING DR ,  MOLZ PETER DR ,  NEUENHOFER STEPHAN DR ,  SCHNORR GERD DR ,  SIMONS GUIDO DR ,  SKRZIPCZYK HEINZ-JUERGEN DR ,  WEIMER KURT E DR

IPC: G01N33/543

Abstract: The invention relates to a process for the stabilization of biologically active substances which are immobilized on a carrier, where the solid phase with the immobilized biologically active substance is contacted for the stabilization with a solution which contains polyanetholesulfonic acid and/or salts thereof. The biologically active substances are, in particular, antibodies.

公开(公告)号：DE3720983A1

公开(公告)日：1989-01-05

申请号：DE3720983

申请日：1987-06-25

Applicant: HOECHST AG

Inventor： HARTHUS HANS PETER DR ,  SCHNORR GERD DR ,  WALTER GOETZ DR ,  STRECKER HELMUT DR ,  MOLZ PETER DR ,  SIMONS GUIDO DR ,  SKRZIPCZYK HANS-JUERGEN DR

IPC: G01N33/53 ,  G01N33/543 ,  G01N33/577 ,  G01N33/68 ,  G01N33/532 ,  G01N33/74