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    11.
    发明专利
    未知

    公开(公告)号：DE69433032T2

    公开(公告)日：2004-06-03

    申请号：DE69433032

    申请日：1994-05-19

    Applicant: KAO CORP

    Inventor： ARA KATSUTOSHI ,  SAEKI KATSUHISA ,  IGARASHI KAZUAKI ,  TAKAIWA MIKIO ,  UEMURA TAKAAKI ,  KAWAI SHUJI ,  ITO SUSUMU ,  HAGIWARA HIROSHI ,  KOBAYASHI TOHRU ,  TANAKA ATSUSHI ,  HOSHINO EIICHI

    IPC: C11D3/386 ,  C12N9/28

    Abstract: A liquefying alkaline alpha -amylase having the following enzymological properties: (1) action: this enzyme hydrolyzes alpha -1,4-glycoside linkages of starch, amylose, amylopectin and partial hydrolyzates thereof, and forms glucose, maltose, maltotriose, maltotetraose, maltopentaose and maltohexaose from amylose, though it does not act upon pullulan; (2) isoelectric point: it has an isolectric point exceeding 8.5 as measured by isoelectric electrophoresis. This enzyme has such a liquefying activity as to hydrolyze starch and starch polysaccharides at high random and has an optimum pH value on an alkaline side. Since it has a high isoelectric point, it can readily be purified. A detergent composition containing this enzyme has an excellent detergency against especially stains due to spilt food.

    Alkaline pullulanase microorganism producing the same and process for producing the same
    12.
    发明专利
    Alkaline pullulanase microorganism producing the same and process for producing the same 未知

    公开(公告)号：HK154695A

    公开(公告)日：1995-10-06

    申请号：HK154695

    申请日：1995-09-28

    Applicant: KAO CORP

    Inventor： ARA KATSUTOSHI ,  IGARASHI KAZUAKI ,  SAEKI KATSUHISA ,  ITO SUSUMU

    IPC: C12N9/44 ,  C12N1/20

    Abstract: A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50 DEG C and is thermally stable up to 40 DEG C. The alkaline pullulanase has also strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with alpha -amylase for producing various monosaccharides.

    14.
    发明专利
    未知

    公开(公告)号：ES2065449T3

    公开(公告)日：1995-02-16

    申请号：ES90116597

    申请日：1990-08-29

    Applicant: KAO CORP

    Inventor： ARA KATSUTOSHI ,  IGARASHI KAZUAKI ,  SAEKI KATSUHISA ,  ITO SUSUMU

    IPC: C12N9/44 ,  C12N1/20

    Abstract: A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50 DEG C and is thermally stable up to 40 DEG C. The alkaline pullulanase has also strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with alpha -amylase for producing various monosaccharides.

    15.
    发明专利
    未知

    公开(公告)号：DE69013446D1

    公开(公告)日：1994-11-24

    申请号：DE69013446

    申请日：1990-08-29

    Applicant: KAO CORP

    Inventor： ARA KATSUTOSHI ,  IGARASHI KAZUAKI ,  SAEKI KATSUHISA ,  ITO SUSUMU

    IPC: C12N9/44 ,  C12N1/20

    Abstract: A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50 DEG C and is thermally stable up to 40 DEG C. The alkaline pullulanase has also strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with alpha -amylase for producing various monosaccharides.

    Modified promoter
    19.
    发明专利
    Modified promoter 未知

    公开(公告)号：AU2010308865B2

    公开(公告)日：2014-08-21

    申请号：AU2010308865

    申请日：2010-10-18

    Applicant: KAO CORP

    Inventor： KAWAHARA AKIHITO ,  KODAMA HIROSHI ,  ARA KATSUTOSHI

    IPC: C12N15/113

    Abstract: The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

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