Abstract:
Detergent compositions containing protopectinase whose optimum pH for the reaction is 7.0 or higher when protopectin or polygalacturonic acid is used as a substrate. The detergent compositions are endowed with remarkable detergency against mud soil.
Abstract:
A liquefying alkaline α-amylase having the following enzymological properties: (1) action: this enzyme hydrolyzes α-1,4-glycoside linkages of starch, amylose, amylopectin and partial hydrolyzates thereof, and forms glucose, maltose, maltotriose, maltotetraose, maltopentaose and maltohexaose from amylose, though it does not act upon pullulan; (2) isoelectric point: it has an isolectric point exceeding 8.5 as measured by isoelectric electrophoresis. This enzyme has such a liquefying activity as to hydrolyze starch and starch polysaccharides at high random and has an optimum pH value on an alkaline side. Since it has a high isoelectric point, it can readily be purified. A detergent composition containing this enzyme has an excellent detergency against especially stains due to spilt food.
Abstract:
A liquefying alkaline alpha -amylase having the following enzymological properties: (1) action: this enzyme hydrolyzes alpha -1,4-glycoside linkages of starch, amylose, amylopectin and partial hydrolyzates thereof, and forms glucose, maltose, maltotriose, maltotetraose, maltopentaose and maltohexaose from amylose, though it does not act upon pullulan; (2) isoelectric point: it has an isolectric point exceeding 8.5 as measured by isoelectric electrophoresis. This enzyme has such a liquefying activity as to hydrolyze starch and starch polysaccharides at high random and has an optimum pH value on an alkaline side. Since it has a high isoelectric point, it can readily be purified. A detergent composition containing this enzyme has an excellent detergency against especially stains due to spilt food.
Abstract:
The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium alpha -olefin sulfonate, sodium alkyl sulfonate, and alpha -sulfo-fatty acid ester. The present invention further provides for a microorganism producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.
Abstract:
Den foreliggende opfindelse angår en alkalisk pratease med en præprosekvens, hvor præpro-sekvensen er en mutantsekvens af aminosyresekvensen i SEQ ID NO: 1, eller en aminosyresekvens med 80 % eller højere homologi med aminosyresekvensen i SEQ ID NO: 1, hvor aminosyrerester i (a) position 52, (b) position 75, og (c) position 142 i SEQ ID NO: 1, eller aminosyrerester i positioner svarende til disse positioner, substitueres med følgende aminosyrerester: i position (a): asparaginsyre eller arginin, i position (b): al an in eller arginin, og i position (c): lysin, og den alkaliske protease, når den er i moden form, har aminosyresekvensen i SEQ ID NO: 2 eller en aminosyresekvens med en homologi på 80 % eller højere med denne aminosyresekvens. Den foreliggende opfindelse angår for eksempel også et gen, der koder for den alkaliske protease. Ifølge den foreliggende opfindelse kan der fremstilles en alkalisk pratease i forøget mængde. Især kan der effektivt fremstilles en alkalisk pratease med aktivitet selv i nærvær af en fedtsyre i høj koncentration og med fremragende renseevne over for komplekse tilsmudsninger indeholdende proteiner og hudfedt.