公开(公告)号：EP2556173A4

公开(公告)日：2013-12-04

申请号：EP11766806

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： CONNER THOMAS A ,  LIM CHEE KIONG ,  PALLAS MICHAEL C ,  CHING CHEE WEE

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12M1/38 ,  G01N33/50

CPC classification number: B01L7/52 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1894

Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.