公开(公告)号：KR20200138242A

公开(公告)日：2020-12-09

申请号：KR20207027752

申请日：2019-02-19

Applicant: LIFE TECHNOLOGIES CORP

Inventor： WISE TRISTAN ,  RHODES CHARLES

IPC: B01L3/00 ,  B01L9/04

Abstract: 물체를고정하기위한위치결정장치는기부판, 부착판, 및고정요소를포함한다. 기부판은부착판의돌출부가미끄러지는가이드를포함하여위치결정장치를개방위치에서폐쇄위치로변경할수 있다. 기부판은또한관형부재를수용하기위한수용부를포함하며, 이수용부는폐쇄위치에서수용부와고정요소에의해움직임이제한된다. 위치결정장치는또한작동중에폐쇄위치를유지하는것을돕는래치를포함할수 있다.

公开(公告)号：KR20200128105A

公开(公告)日：2020-11-11

申请号：KR20207028036

申请日：2019-03-01

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MULLAH KHAIRUZZAMAN BASHAR ,  EVANS BRIAN ,  BENSON SCOTT C ,  CHANG CHU AN ,  YAN XIONGWEI

IPC: C12Q1/6818 ,  C09B11/24 ,  C12Q1/686

Abstract: 생물학적검정법에사용되는프로브가개시된다. 프로브는올리고뉴클레오티드링커를통해소광제화합물에결합된형광염료를포함한다. 또한정량적 PCR 반응 (qPCR)에서와같은예컨대중합효소연쇄반응 (PCR)용프로브를사용하는방법및 상기프로브를포함하는키트가개시된다.

公开(公告)号：KR20180030613A

公开(公告)日：2018-03-23

申请号：KR20187003996

申请日：2016-07-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： ZMUDA JONATHAN ,  LIU CHAO YAN

IPC: C12P21/02 ,  C07K14/505 ,  C07K16/00 ,  C12N5/00

CPC classification number: C12P21/02 ,  C07K14/505 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/92 ,  C12N5/0043 ,  C12N2510/02 ,  C12N2511/00

Abstract: 본발명은일반적으로현탁 CHO 세포중의재조합단백질의높은수준의발현에적합한시스템및 방법에관한것이다. 특히, 본발명은본 발명의도입이상기과정동안에성장배지를대체하거나재충전하거나보출할필요를제거하게끔한다. 본발명은또한현탁 CHO 세포를형질전환/형질감염시키는데유용한조성물및 키트에관한것이다.

Abstract translation: 本发明一般涉及适用于在悬浮的CHO细胞中高水平表达重组蛋白的系统和方法。 具体而言，本发明消除了在本发明的引入阶段期间替换，再充注或分配生长培养基的需要。 本发明还涉及用于转化/转染悬浮CHO细胞的组合物和试剂盒。

公开(公告)号：KR20180023019A

公开(公告)日：2018-03-06

申请号：KR20187004947

申请日：2011-04-22

Applicant: LIFE TECHNOLOGIES CORP

Inventor： BARRETT SHAWN ,  JACOBIA SCOTT

IPC: C12N5/00 ,  C12P21/02

CPC classification number: C12N5/0018 ,  C12N5/005 ,  C12N2500/32 ,  C12N2501/998 ,  C12N2510/02 ,  C12P21/02

Abstract: 세포배양배지, 농축배지, 및공급물, 세포배양배지및 공급물을제조하는방법, 및세포를배양하는방법을제공한다. 디펩티드를비롯한, 하나이상의소형펩티드를세포배양배지에첨가함으로써안정성을개선시키고, 세포배양조건을개선시킨다.

Abstract translation: 提供了细胞培养基，浓缩培养基和饲料，制造细胞培养基和饲料的方法以及培养细胞的方法。 将一种或多种小肽（包括二肽）添加到细胞培养基中以提供改进的稳定性和改善培养细胞的条件。

公开(公告)号：JP2014057954A

公开(公告)日：2014-04-03

申请号：JP2013210859

申请日：2013-10-08

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： JONATHAN SCHULTZ ,  DAVID MARRAN

IPC: B01J19/00 ,  G01N37/00

CPC classification number: B01L3/502715 ,  B01J2219/00389 ,  B01J2219/00394 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502769 ,  B01L3/502776 ,  B01L3/52 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12Q1/6869 ,  G01N27/447 ,  G01N35/1002 ,  G01N35/1097 ,  Y10T137/0318 ,  Y10T137/0424 ,  Y10T137/85938 ,  C12Q2565/629 ,  C12Q2527/119

Abstract: PROBLEM TO BE SOLVED: To provide a passive fluidics circuit capable of directing different fluids toward a common volume such as a reaction chamber or flow cell, etc. without entailing the mixing or cross contamination thereof.SOLUTION: Directions and velocities of flows streaming through junctions 335, nodal points 301, and passages of a fluidics circuit 302 are controlled depending on the opening or closure of an upstream valve, fluid pressure differences, flow path resistances, etc. of inlets 304, 306, 308, 310, and/or 312 of the circuit or an upstream storage unit. The free diffusion or leak of a fluid from a non-selected inlet in relation to a common outlet or another inlet specific to a junction or nodal point is prevented by the flow of the fluid through a selected inlet, whereas a portion of the flow of the fluid through the selected inlet passes through the vicinity of the non-selected fluid inlet and streams out of the fluidics circuit past a refuse port 345. A barrier against undesirable mixing with an outlet flow due to leak or diffusion is thus formed.

Abstract translation: 要解决的问题：提供一种能够将不同流体导向诸如反应室或流通池等常见体积的被动流体回路，而不会引起混合或交叉污染。解决方案：流过连接点的流动方向和速度 335，节点301和流体回路302的通道根据上游阀的打开或关闭，入口304,306,308,310和/或312的流体压力差，流动阻力等进行控制 的电路或上游存储单元。 相对于公共出口或连接点或节点特有的另一入口，流体从未选择的入口的自由扩散或泄漏被流体流过选定的入口而被阻止，而一部分流动 通过所选择的入口的流体通过未选择的流体入口附近，并流出流体回路经过垃圾端口345.因此形成了由于泄漏或扩散而与出口流不期望的混合的屏障。

公开(公告)号：JP2013064746A

公开(公告)日：2013-04-11

申请号：JP2012246417

申请日：2012-11-08

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： JONATHAN M ROTHBERG ,  WOLFGANG HINZ ,  KIM L JOHNSON ,  JAMES BUSTILLO

IPC: G01N27/414 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/28 ,  G01N27/416

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: PROBLEM TO BE SOLVED: To provide a method and an apparatus relating to a large scale FET for measuring an analyte.SOLUTION: ChemFET (e.g., ISFET) arrays may be manufactured by using conventional CMOS processing technique based on an improved FET pixel and array design that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel size and high-density arrays. Such arrays may be employed to detect presence and/or concentration change of an analyte of various types in a variety of chemical and/or biological processes. In one example, the chemFET arrays facilitate DNA sequencing technique based on monitoring change in hydrogen ion concentration (pH), change in other analyte concentration, and/or binding events associated with the chemical processes relating to DNA synthesis.

公开(公告)号：JP2013064744A

公开(公告)日：2013-04-11

申请号：JP2012246415

申请日：2012-11-08

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： JONATHAN M ROTHBERG ,  WOLFGANG HINZ ,  KIM L JOHNSON ,  JAMES BUSTILLO

IPC: G01N27/414 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/28

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: PROBLEM TO BE SOLVED: To provide a method and an apparatus relating to a large scale FET for measuring an analyte.SOLUTION: ChemFET (e.g., ISFET) arrays may be manufactured by using conventional CMOS processing technique based on an improved FET pixel and array design that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel size and high-density arrays. Such arrays may be employed to detect presence and/or concentration change of an analyte of various types in a variety of chemical and/or biological processes. In one example, the chemFET arrays facilitate DNA sequencing technique based on monitoring change in hydrogen ion concentration (pH), change in other analyte concentration, and/or binding events associated with the chemical processes relating to DNA synthesis.

公开(公告)号：JP2011036256A

公开(公告)日：2011-02-24

申请号：JP2010201569

申请日：2010-09-09

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： BENNETT ROBERT P ,  WELCH PETER J ,  HARWOOD STEVEN ,  MADDEN KNUT ,  FRIMPONG KENNETH ,  FRANKE KENNETH E

IPC: C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P31/18 ,  C12N1/00 ,  C12N5/10 ,  C12N7/00 ,  C12N15/86 ,  C12N15/861 ,  C12N15/863 ,  C12N15/864 ,  C12N15/867 ,  C12N15/869 ,  C12P21/02

CPC classification number: C12N15/86 ,  C12N2710/10343 ,  C12N2710/14143 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/70 ,  C12N2830/00 ,  C12N2830/003 ,  C12N2830/15 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/20

Abstract: PROBLEM TO BE SOLVED: To provide compositions and methods for the construction of nucleic acids comprising all or a portion of a viral genome. SOLUTION: The recombinant viral genome may be constructed to contain multiple recombination and/or topoisomerase recognition sites. The compositions include vectors having multiple recombination sites with unique specificity that contain all or a portion of a viral genome. The methods permit the insertion of a sequence of interest into a viral genome using recombinational and/or topoisomerase-mediated cloning. There are also provided methods of constructing recombinant virus, methods of expressing polypeptides, and methods of expressing fusion polypeptides. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：提供构建包含病毒基因组全部或部分的核酸的组合物和方法。 解决方案：可以构建重组病毒基因组以含有多个重组和/或拓扑异构酶识别位点。 组合物包含具有独特特异性的多个重组位点的载体，其含有病毒基因组的全部或一部分。 该方法允许使用重组和/或拓扑异构酶介导的克隆将感兴趣的序列插入到病毒基因组中。 还提供了构建重组病毒的方法，表达多肽的方法和表达融合多肽的方法。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010048812A

公开(公告)日：2010-03-04

申请号：JP2009239956

申请日：2009-10-19

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： NAASANI IMAD

IPC: G01N21/78 ,  G01N33/543 ,  B82B1/00 ,  C01B19/04 ,  C09K11/08 ,  G01N33/58

CPC classification number: B82Y15/00 ,  G01N33/588 ,  Y10T428/25 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2998 ,  Y10T428/31721

Abstract: PROBLEM TO BE SOLVED: To provide a new compositional compound having nanocrystals provided with functional and higher fluorescence property. SOLUTION: Compositions of functionalized, fluorescent nanocrystals have fluorescent nanocrystals coated with an imidazole-containing compound, composition of functionalized, fluorescent nanocrystals having fluorescent nanocrystals coated with the imidazole-containing compound and cross-linked with a phosphine cross-linking compound, composition of functionalized, fluorescent nanocrystals having effective chemical bond with molecular probe, a process to produce the functionalized, fluorescent nanocrystals, and a process to utilize the functionalized, fluorescent nanocrystals in a detecting device. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供具有功能和更高荧光特性的具有纳米晶体的新的组成化合物。 官能化的荧光纳米晶体的组合物具有用含咪唑的化合物涂覆的荧光纳米晶体，具有涂覆有含咪唑的化合物并与膦交联化合物交联的荧光纳米晶体的官能化的荧光纳米晶体的组合物， 具有与分子探针有效化学键的官能化荧光纳米晶体的组成，产生官能化荧光纳米晶体的方法，以及在检测装置中利用官能化的荧光纳米晶体的方法。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009244270A

公开(公告)日：2009-10-22

申请号：JP2009118156

申请日：2009-05-15

Applicant: Life Technologies Corp ,  ライフ テクノロジーズ コーポレーション

Inventor： YGUERABIDE JUAN ,  YGUERABIDE EVANGELINA ,  WARDEN LAURENCE ,  PETERSON TODD

IPC: G01N15/00 ,  G01N21/64 ,  C12Q1/68 ,  G01N15/14 ,  G01N21/27 ,  G01N21/47 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N37/00

CPC classification number: G01N15/1468 ,  C12Q1/6816 ,  G01N21/47 ,  G01N2021/6439 ,  C12Q2565/501 ,  C12Q2563/137

Abstract: PROBLEM TO BE SOLVED: To provide a diagnosis measurement method by light scatter which has ease of use and high detection sensitivity, and allows analyte measurement to be applied to a wide range of concentrations. SOLUTION: The methods are for enhancing a dynamic range for specific detection of one or a plurality of analytes in assays using scattered-light detectable particle labels, and are used together with resonance light scattering particle labels. Integrated light is detected for two or more exposure times so as to be in proportion to integrated light intensity, and signals of them are synthesized and the light is quantitated so as to enhance the dynamic range to either or both of lower and higher concentrations. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供易于使用和高检测灵敏度的光散射的诊断测量方法，并且允许将分析物测量应用于宽范围的浓度。 解决方案：该方法用于增强用于使用散射光可检测的颗粒标记的测定中的一种或多种分析物的特异性检测的动态范围，并且与共振光散射粒子标签一起使用。 检测到两个或更多个曝光时间的集成光，以便与积分光强度成比例，并且合成它们的信号，并对光进行定量，以便增强低浓度和较高浓度之一或两者的动态范围。 版权所有（C）2010，JPO＆INPIT