公开(公告)号：US09115397B2

公开(公告)日：2015-08-25

申请号：US14488751

申请日：2014-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: C07D215/00 ,  C12Q1/68 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  G01N21/64

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

公开(公告)号：US08962853B2

公开(公告)日：2015-02-24

申请号：US13975675

申请日：2013-08-26

Applicant: Life Technologies Corporation

Inventor： Ching-Ying Cheung ,  Diane Gray ,  Stephen Yue

IPC: C07D291/00 ,  C07C211/00 ,  G01N33/58 ,  C07D211/62 ,  C07D295/112 ,  C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C12Q1/04 ,  C12Q1/18 ,  G01N33/52

CPC classification number: G01N33/1806 ,  C07D211/62 ,  C07D295/112 ,  C07D401/14 ,  C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/643 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6432

Abstract: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

Abstract translation: 公开了含有荧光团和可还原淬火单元的化学染料化合物。 可还原淬火单元在处于氧化状态时淬灭荧光团。 在还原时，淬火单元的淬火特性减弱或消除。 化合物可用于各种应用，包括检测细菌细胞，监测细菌细胞的电子传递链功能，监测非生物系统的氧化状态，以及测定抗菌剂或抗菌剂的有效性。

公开(公告)号：US20140094608A1

公开(公告)日：2014-04-03

申请号：US14030835

申请日：2013-09-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wai-Yee LEUNG ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D498/04 ,  C07D417/06 ,  C07D471/04 ,  C07D209/18

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。