公开(公告)号：US11175204B1

公开(公告)日：2021-11-16

申请号：US15192107

申请日：2016-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C07D311/00 ,  G01N1/30 ,  C07D405/12 ,  C07D405/14 ,  G01N33/50

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

公开(公告)号：US09366676B2

公开(公告)日：2016-06-14

申请号：US14797415

申请日：2015-07-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: C07D215/00 ,  G01N33/58 ,  G01N21/64 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/68

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

公开(公告)号：US09115397B2

公开(公告)日：2015-08-25

申请号：US14488751

申请日：2014-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: C07D215/00 ,  C12Q1/68 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  G01N21/64

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

公开(公告)号：US08865904B2

公开(公告)日：2014-10-21

申请号：US13861123

申请日：2013-04-11

Applicant: Life Technologies Corporation

Inventor： Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: C07D215/00 ,  C07D417/04 ,  C07D215/10 ,  C07D277/72 ,  C07D277/74 ,  G01N21/64 ,  C07D417/14 ,  C07D417/06 ,  C07D215/227

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。

公开(公告)号：US09400273B1

公开(公告)日：2016-07-26

申请号：US14801000

申请日：2015-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C12Q1/02 ,  G01N33/50 ,  C07D405/14 ,  C07D405/12

CPC classification number: G01N33/5008 ,  C07D405/12 ,  C07D405/14 ,  G01N33/533 ,  G01N2430/00

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

Abstract translation: 本文描述了用于长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本发明的化合物是能够以405nm紫激光激发的新型细胞追踪试剂，能够提供明亮的荧光强度，均匀的细胞染色和细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：US10138510B2

公开(公告)日：2018-11-27

申请号：US14300728

申请日：2014-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Clarke ,  Jolene Bradford

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6804

Abstract: The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

公开(公告)号：US09709557B2

公开(公告)日：2017-07-18

申请号：US14632168

申请日：2015-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Yue ,  Shih-Jung Huang ,  Jolene Bradford

IPC: G01N33/52 ,  C12Q1/68 ,  C09B1/516 ,  C07H21/04 ,  G01N21/64

CPC classification number: G01N33/52 ,  C07C225/36 ,  C07C237/10 ,  C07C2603/24 ,  C07D205/04 ,  C07D213/36 ,  C07D233/61 ,  C07D249/04 ,  C07D295/13 ,  C07D295/192 ,  C07H21/04 ,  C09B1/5165 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N21/6428 ,  G01N2021/6441

Abstract: Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

公开(公告)号：US08822695B2

公开(公告)日：2014-09-02

申请号：US13777910

申请日：2013-02-26

Applicant: Life Technologies Corporation

Inventor： Gayle Buller ,  Jixiang Liu ,  Stephen Yue ,  Jolene Bradford

IPC: C07D409/04 ,  G01N33/52

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

Abstract translation: 本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。 这些染料可用于检测样品中的分析物和染料缀合物的制备。