公开(公告)号：US10738353B2

公开(公告)日：2020-08-11

申请号：US16120007

申请日：2018-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US09139667B2

公开(公告)日：2015-09-22

申请号：US13763066

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C08F8/40 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂抗衡离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。

公开(公告)号：US11578356B2

公开(公告)日：2023-02-14

申请号：US16938192

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

公开(公告)号：US10914735B2

公开(公告)日：2021-02-09

申请号：US15846035

申请日：2017-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Giorda

IPC: G01N33/543 ,  G01N33/569 ,  G01N35/00 ,  C12Q1/6876 ,  G01N33/68

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

公开(公告)号：US10093972B2

公开(公告)日：2018-10-09

申请号：US15166372

申请日：2016-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C07K14/00 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US20180100194A1

公开(公告)日：2018-04-12

申请号：US15846035

申请日：2017-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Giorda

IPC: C12Q1/6876 ,  G01N33/543 ,  G01N33/569 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

公开(公告)号：US09422384B2

公开(公告)日：2016-08-23

申请号：US14745815

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20150203912A1

公开(公告)日：2015-07-23

申请号：US14410886

申请日：2013-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Glorda

IPC: C12Q1/68 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

Abstract translation: 因此，在一些实施例中，提供了用于检测一个或多个样品中的分析物或分析物的方法。 所述方法包括提供具有可寻址标记和相关配体的固体支持物，使固体基质接触样品，从而形成接触的固体支持物，将接触的固体支持物与FET阵列相关联并检测FET阵列的电特性， 从而检测一个或多个样品中的分析物或分析物。 在其他实施例中，样本包含第二可寻址标记。

公开(公告)号：US09067954B2

公开(公告)日：2015-06-30

申请号：US14081263

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08L9/00 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09062080B2

公开(公告)日：2015-06-23

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F236/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.