公开(公告)号：US10738353B2

公开(公告)日：2020-08-11

申请号：US16120007

申请日：2018-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20150191775A1

公开(公告)日：2015-07-09

申请号：US14593858

申请日：2015-01-09

Applicant: Life Technologies Corporation

Inventor： Lei XI ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。

公开(公告)号：US11578356B2

公开(公告)日：2023-02-14

申请号：US16938192

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

公开(公告)号：US09476093B2

公开(公告)日：2016-10-25

申请号：US14593858

申请日：2015-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei Xi ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C12N15/10

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.