公开(公告)号：US09709557B2

公开(公告)日：2017-07-18

申请号：US14632168

申请日：2015-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Yue ,  Shih-Jung Huang ,  Jolene Bradford

IPC: G01N33/52 ,  C12Q1/68 ,  C09B1/516 ,  C07H21/04 ,  G01N21/64

CPC classification number: G01N33/52 ,  C07C225/36 ,  C07C237/10 ,  C07C2603/24 ,  C07D205/04 ,  C07D213/36 ,  C07D233/61 ,  C07D249/04 ,  C07D295/13 ,  C07D295/192 ,  C07H21/04 ,  C09B1/5165 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N21/6428 ,  G01N2021/6441

Abstract: Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

公开(公告)号：US20150218379A1

公开(公告)日：2015-08-06

申请号：US14402358

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  G01N33/58 ,  G01N33/50 ,  G01N33/84

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。

公开(公告)号：US20130224735A1

公开(公告)日：2013-08-29

申请号：US13861123

申请日：2013-04-11

Applicant: Life Technologies Corporation

Inventor： Jolene BRADFORD ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: G01N21/64

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

公开(公告)号：US20240042062A1

公开(公告)日：2024-02-08

申请号：US18380388

申请日：2023-10-16

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： Surbhi Desai ,  Marie Christine Nlend ,  Kyle Gee ,  Matthew Baker ,  Robert Aggeler ,  Scott Sweeney ,  Aleksey Rukavishnikov ,  Shih-Jung Huang

IPC: A61K49/00 ,  G01N33/58 ,  A61K47/10 ,  A61K47/18 ,  G01N33/533 ,  G01N33/68

CPC classification number: A61K49/0015 ,  G01N33/582 ,  A61K47/10 ,  A61K47/18 ,  A61K49/0058 ,  G01N33/533 ,  G01N33/68

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.