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公开(公告)号：WO2012065043A9
公开(公告)日：2012-12-20
申请号：PCT/US2011060338
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
Inventor： FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了用于表征修饰的核酸的方法，组合物和系统。 在某些优选的实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰包括化学修饰的碱基，酶修饰的碱基，无碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：WO2012065043A3
公开(公告)日：2012-08-16
申请号：PCT/US2011060338
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
Inventor： FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：WO2011139344A3
公开(公告)日：2012-04-05
申请号：PCT/US2011000747
申请日：2011-04-27
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , TURNER STEPHEN , KUSE RON , KEARNS GREGORY , MONADGEMI PEZHMAN , FOQUET MATHIEU , MARTINEZ DREW
Inventor： TURNER STEPHEN , KUSE RON , KEARNS GREGORY , MONADGEMI PEZHMAN , FOQUET MATHIEU , MARTINEZ DREW
CPC classification number: B82Y30/00 , B01J19/0046 , B01J2219/00317 , B01J2219/00509 , B01J2219/00637 , B01J2219/00725 , B01J2219/0074 , B01L3/5085 , B01L2200/0668 , B01L2200/12 , B01L2300/0819 , B01L2300/0896
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided. Arrays of confined regions are produced wherein each confined region comprises a single island within the confined region. The island can be selectively functionalized with a coupling agent to couple a single molecule of interest within the confined region.
Abstract translation: 提供了将单一分析物分子非随机加载到阵列结构中的方法，组合物和阵列。 产生限制区域的阵列，其中每个限制区域包括在限制区域内的单个岛。 岛可以用偶联剂选择性地官能化，以将约束区域内的单个感兴趣的分子耦合。
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14.发明申请METHODS AND SYSTEMS FOR SIMULTANEOUS REAL-TIME MONITORING OF OPTICAL SIGNALS FROM MULTIPLE SOURCES 审中-公开用于同时实时监测来自多个源的光信号的方法和系统
公开(公告)号：WO2007095235A3
公开(公告)日：2008-06-19
申请号：PCT/US2007003804
申请日：2007-02-09
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , LUNDQUIST PAUL , ZACCARIN DENIS , LACROIX YVES , TURNER STEPHEN , DIXON JOHN
Inventor： LUNDQUIST PAUL , ZACCARIN DENIS , LACROIX YVES , TURNER STEPHEN , DIXON JOHN
IPC: G01J3/30
CPC classification number: G01N21/6452 , G01J3/02 , G01J3/0224 , G01J3/10 , G01J3/14 , G01J3/2803 , G01N21/6445 , G01N21/6456 , G01N21/648 , G01N2021/6417 , G01N2021/6441
Abstract: Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.
Abstract translation: 用于实时监控来自信号源阵列的光信号的方法和系统，特别是具有光谱不同信号分量的光信号源。 系统包括与光链路进行光通信的信号源阵列，该信号源阵列将激励辐射引导到这些阵列并从这些阵列发射信号，并将这些信号成像到检测器阵列上，从这些信号可以进行额外的处理。
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公开(公告)号：WO2010068289A3
公开(公告)日：2010-10-14
申请号：PCT/US2009006517
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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Patent Agency Ranking
公开(公告)号：WO2009045344A3
公开(公告)日：2009-07-09
申请号：PCT/US2008011206
申请日：2008-09-26
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , HEINER CHERYL , TURNER STEPHEN , TRAVERS KEVIN
Inventor： HEINER CHERYL , TURNER STEPHEN , TRAVERS KEVIN
CPC classification number: C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
Abstract: Methods of preparing nucleic acid templates and providing the nucleic acid templates to low copy number reaction volumes are provided. Related compositions of nucleic acid templates are also provided.
Abstract translation: 提供了制备核酸模板并将核酸模板提供给低拷贝数反应体积的方法。 还提供了核酸模板的相关组合物。
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公开(公告)号：WO2006086673A3
公开(公告)日：2009-05-28
申请号：PCT/US2006004810
申请日：2006-02-09
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , TURNER STEPHEN , KORLACH JONAS
Inventor： TURNER STEPHEN , KORLACH JONAS
IPC: C12P19/34
CPC classification number: C12Q1/6874 , C12P19/34 , C12Q1/6806 , C12Q1/6813 , C12Q1/6869 , C12Q2533/101 , C12Q2521/101 , C12Q2527/127 , C12Q2521/319
Abstract: The present invention relates to preparation of nucleotide compositions and uses thereof for conducting nucleic acid analyses. The compositions and methods embodied in the present invention are particularly useful for nucleic acid analyses that require high-resolution detection of labeled nucleotides or labeled nucleic acid targets.
Abstract translation: 本发明涉及核苷酸组合物的制备及其用于进行核酸分析的用途。 本发明中体现的组合物和方法对于需要高分辨率检测标记的核苷酸或标记的核酸靶标的核酸分析特别有用。
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18.发明申请
公开(公告)号：WO2009038668A3
公开(公告)日：2009-05-22
申请号：PCT/US2008010652
申请日：2008-09-12
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , LUNDQUIST PAUL , ZHONG CHENG FRANK , ZHAO PEGIAN , TURNER STEPHEN
Inventor： LUNDQUIST PAUL , ZHONG CHENG FRANK , ZHAO PEGIAN , TURNER STEPHEN
IPC: G01N21/64
CPC classification number: G01N27/44721
Abstract: Systems and methods for analyzing highly multiplexed sample arrays using highly multiplexed, high density optical systems to illuminate high density sample arrays and/or provide detection and preferably confocal detection off signals emanating from such high density arrays. Systems and methods are applied in a variety of different analytical operations, including analysis of biological and biochemical reactions, including nucleic acid synthesis and derivation of sequence information from such synthesis.
Abstract translation: 用于使用高度多路复用的高密度光学系统来分析高度多路复用的样本阵列以照射高密度样本阵列和/或提供从这样的高密度阵列发出的检测并且优选地共焦检测关闭信号的系统和方法。 系统和方法应用于各种不同的分析操作，包括生物和生物化学反应的分析，包括核酸合成和从这种合成得到的序列信息。
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19.发明申请PROTEIN ENGINEERING STRATEGIES TO OPTIMIZE ACTIVITY OF SURFACE ATTACHED PROTEINS 审中-公开蛋白质工程策略优化表面附着蛋白的活性
公开(公告)号：WO2007075873A3
公开(公告)日：2007-11-29
申请号：PCT/US2006048764
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , HANZEL DAVID , KORLACH JONAS , PELUSO PAUL , OTTO GEOFF , PHAM THANG , RANK DAVID , TURNER STEPHEN
Inventor： HANZEL DAVID , KORLACH JONAS , PELUSO PAUL , OTTO GEOFF , PHAM THANG , RANK DAVID , TURNER STEPHEN
CPC classification number: C12N9/22 , C07K2319/21 , Y10T428/2991 , Y10T428/2993 , Y10T428/2996 , Y10T428/31663 , Y10T428/31768
Abstract: Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.
Abstract translation: 提供了包括表面结合结构域，具有与其结合的活性酶的表面和将酶偶联到表面的方法的分离和/或重组酶。 酶可以包括用于表面偶联的大和/或多个表面偶联域。
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20.发明申请IMPROVED METHODS, SYSTEMS AND COMPOSITIONS FOR MONITORING ENZYME ACTIVITY AND APPLICATIONS THEREOF 审中-公开用于监测酶活性的改进方法，系统和组合物及其应用
公开(公告)号：WO2007115205A2
公开(公告)日：2007-10-11
申请号：PCT/US2007065687
申请日：2007-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , KORLACH JONAS , TURNER STEPHEN
Inventor： KORLACH JONAS , TURNER STEPHEN
IPC: G01C21/32
CPC classification number: C12Q1/66 , C12Q1/42 , G01N33/573
Abstract: Methods of monitoring enzyme mediated reactions, and particularly nucleic acid synthesis reactions such as pyrosequencing methods that employ enzymatic reporter systems. The methods and systems provide elevated signal levels as compared to conventional pyrosequencing processes, and/or mediate de-phasing of sequencing analyses employing pyrosequencing or other "sequencing by synthesis" methods.
Abstract translation: 监测酶介导的反应的方法，特别是核酸合成反应，例如采用酶报告系统的焦磷酸测序方法。 与常规焦磷酸测序方法相比，该方法和系统提供升高的信号水平，和/或介导使用焦磷酸测序或其他“合成测序”方法进行测序分析的脱相。
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