公开(公告)号：EP1099107B1

公开(公告)日：2009-01-07

申请号：EP99934902.0

申请日：1999-07-20

Applicant: PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64 ,  G01N21/76

CPC classification number: G01N21/6452 ,  G01N21/76 ,  G01N2021/6421 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833 ,  G01N2201/0866

Abstract: Apparatus for detecting light emitted by assay samples is provided, in which light emitted by the sample is collected for transmission to a charge coupled device camera (74) by an optical fibre bundle. The cross-sectional area of the optical fibre bundle corresponds to the area of the sample, the end of which is located close to the sample for detecting any light emitted therefrom, and selected fibres (30) of those making up the bundle are separated from the remainder and extend to a source of excitation radiation (76) and serve to convey excitation radiation (if required) directly to a corresponding plurality of points distributed over the area of the end face of the bundle and therefore over the area of the sample. The remaining fibres (32, 38) of the bundle serve to collect emitted light (whether generated by fluorescence caused by excitation or otherwise) and provide a light path to the charge coupled device camera, wherein the ends of the excitation fibres and the ends of the emitted light collecting fibres are distributed uniformly over the area of the fibre bundle presented to the reaction site.

公开(公告)号：EP1166087B1

公开(公告)日：2004-11-10

申请号：EP00901211.3

申请日：2000-01-25

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBROOKE, John Gordon, 10 Barrington House ,  HOOPER, Claire, Elizabeth

IPC: G01N21/25 ,  G02B6/06

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G02B6/4206 ,  G02B6/4298

Abstract: An optical system for imaging a multiwell sample plate onto a CCD camera, wherein light from the illuminated sample plate (26) is imaged by one or more lenses (20, 24) onto a fibre optic taper (22), bonded to the input face of the camera (28).

公开(公告)号：EP1348120A1

公开(公告)日：2003-10-01

申请号：EP01272703.8

申请日：2001-12-18

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G01N21/76 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fibre optic epi-fluorescence imaging system in which the optical fibres are rearranged so that the system can be used for measuring luminescence samples. The system comprises at least two optical fibres (32, 46) or bundles of fibres which lead to a CCD camera (74), the fibres or bundles of fibres from all samples being arranged in two sets, a first set which are formed from a non-fluorescing material and a second set which are formed from a material which may fluoresce but enables the fibres formed therefrom to have a higher numerical aperature than those of the first set.

公开(公告)号：EP1190232A1

公开(公告)日：2002-03-27

申请号：EP00922798.4

申请日：2000-04-20

Applicant: Packard Instrument Company, Inc.

Inventor： RUSHBOOKE, John Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6458 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0833

Abstract: A method and apparatus for the measurement of radiation, especially fluorescence from samples in assays, wherein a plurality of micro-sample light emitting sites are imaged simultaneously onto a detector array by a plurality of miniature objectives, one for each sample site and focussed thereon, producing parallel beams of light arranged in parallel and spaced apart, which beams are focussed at a pinhole aperture and then reconstituted as parallel beams for incidence on the detector array.

Abstract translation: 用于测量辐射的方法和装置，特别是在测定中来自样品的荧光，其中通过多个微型物镜将多个微样品发光位点同时成像到检测器阵列上，每个样品位置一个，并且聚焦在其上， 产生平行和间隔布置的平行光束，这些光束被聚焦在针孔上，然后被重构为平行光束以入射在检测器阵列上。

公开(公告)号：EP1068513B1

公开(公告)日：2002-01-09

申请号：EP99910524.0

申请日：1999-03-18

Applicant: PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth ,  TOMISEK, John, David

IPC: G01N21/64 ,  G01N33/542 ,  G01N33/58

CPC classification number: G01N21/6452 ,  G01N21/274 ,  G01N21/6428

Abstract: Method of performing a biomedical assay comprising the steps of: exciting the sample or samples with incident radiation of a given wavelength, thereby causing the sample to emit radiation of a different wavelength, measuring the quantity of light falling on a detector receiving the emitted radiation from the sample, thereby to produce a first measurement, illuminating the sample or samples with incident radiation in a manner which does not cause the sample to emit any significant radiation, again detecting the quantity of light falling on the detector, thereby to produce a second measurement, and correcting the first measurement with respect to the second measurement.