公开(公告)号：EP3715831A1

公开(公告)日：2020-09-30

申请号：EP20150539.3

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard

IPC: G01N21/64 ,  G01N21/77 ,  B01L3/00

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

公开(公告)号：EP3619323A1

公开(公告)日：2020-03-11

申请号：EP18794185.1

申请日：2018-05-04

Applicant: Quantum-Si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  LACKEY, Jeremy ,  CHEN, Guojun

IPC: C12Q1/68 ,  C12M1/00 ,  C12M1/34

公开(公告)号：EP3194935B1

公开(公告)日：2018-10-31

申请号：EP15759983.8

申请日：2015-08-07

Applicant: Quantum-si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  FIFE, Keith G. ,  BOISVERT, David

IPC: G01N21/64 ,  G01S17/89

CPC classification number: G01N21/6428 ,  C12Q1/6869 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6458 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/06113 ,  G01N2201/0697 ,  G01S7/4863 ,  G01S7/4865 ,  H01L27/14603 ,  H01L27/14687 ,  H01L27/14812

Abstract: An integrated circuit includes a photodetection region configured to receive incident photons. The photodetection region is configured to produce a plurality of charge carriers in response to the incident photons. The integrated circuit also includes at least one charge carrier storage region. The integrated circuit also includes a charge carrier segregation structure configured to selectively direct charge carriers of the plurality of charge carriers into the at least one charge carrier storage region based upon times at which the charge carriers are produced.

公开(公告)号：EP3194935A2

公开(公告)日：2017-07-26

申请号：EP15759983.8

申请日：2015-08-07

Applicant: Quantum-si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  FIFE, Keith G. ,  BOISVERT, David

IPC: G01N21/64 ,  G01S17/89

CPC classification number: G01N21/6428 ,  C12Q1/6869 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6458 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/06113 ,  G01N2201/0697 ,  G01S7/4863 ,  G01S7/4865 ,  H01L27/14603 ,  H01L27/14687 ,  H01L27/14812

Abstract: An integrated circuit includes a photodetection region configured to receive incident photons. The photodetection region is configured to produce a plurality of charge carriers in response to the incident photons. The integrated circuit also includes at least one charge carrier storage region. The integrated circuit also includes a charge carrier segregation structure configured to selectively direct charge carriers of the plurality of charge carriers into the at least one charge carrier storage region based upon times at which the charge carriers are produced.

Abstract translation: 集成电路包括配置成接收入射光子的光电探测区域。 光检测区域被配置成响应于入射光子而产生多个电荷载流子。 该集成电路还包括至少一个电荷载流子存储区域。 该集成电路还包括电荷载流子分离结构，该电荷载流子分离结构被配置为基于产生电荷载流子的时间选择性地将多个电荷载流子的电荷载流子导入至少一个电荷载流子存储区域。

公开(公告)号：EP3194934A1

公开(公告)日：2017-07-26

申请号：EP15753565.9

申请日：2015-08-07

Applicant: Quantum-si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard ,  WEST, Lawrence C. ,  CIPRIANY, Benjamin ,  FIFE, Keith G. ,  GHASEMI, Farshid

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6408 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/648 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/0696 ,  G01N2201/08 ,  G01N2201/12

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.

Abstract translation: 用于分析单个分子并进行核酸测序的系统和方法。 集成装置包括具有被配置为接收样本的样本井的多个像素，所述样本在被激发时发射辐射。 集成装置包括至少一个波导，其被配置为将激发能量从集成装置的被配置为与激发能量源耦合的区域传播到样品阱。 像素还可以包括用于将发射能量引导至像素内的传感器的至少一个元件。 该系统还包括一个与集成设备连接的仪器。 该仪器可以包括激发能量源，用于通过耦合到集成装置的激发能量耦合区域来向集成装置提供激发能量。 可以通过发射能量的时间参数区分的多个标记中的一个可以标记样本，并且像素内的传感器的配置可以允许检测与标记样本的标记相关联的时间参数。

公开(公告)号：EP4421188A2

公开(公告)日：2024-08-28

申请号：EP24161226.6

申请日：2015-08-07

Applicant: Quantum-Si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard ,  WEST, Lawrence C. ,  CIPRIANY, Benjamin ,  FIFE, Keith G. ,  GHASEMI, Farshid

IPC: C12Q1/6869

CPC classification number: C12Q1/6874 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/648 ,  G01N2021/641920130101 ,  G01N2021/642120130101 ,  G01N21/6408 ,  C12Q1/6869 ,  G01N2021/644120130101 ,  G01N2201/06420130101 ,  G01N2021/647120130101

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.

公开(公告)号：EP4009330A1

公开(公告)日：2022-06-08

申请号：EP21199556.8

申请日：2017-06-01

Applicant: Quantum-Si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  WENGER, Craig ,  DAVEY, Mel ,  FIFE, Keith G. ,  JIA, Jimmy ,  REED, Brian ,  GYARFAS, Brett J.

IPC: G16B30/10 ,  G01N21/64

Abstract: System and methods for identifying nucleotides based on data acquired from a sensor during sequencing of nucleic acids. The method may include obtaining characteristics of light detected from luminescent labels associated with the nucleotides during nucleotide incorporation events. The characteristics may include, for each nucleotide incorporation event, a temporal characteristic the light and an intensity characteristic of the light. The temporal characteristic representing a speed of decay of a probability of photon emission by a luminescent label after excitation. The method may further include grouping points representing the characteristics of the nucleotide incorporation events into groups of points. The individual points may represent at least the temporal characteristic and the intensity characteristic for a corresponding nucleotide incorporation event. The method may further include assigning the groups of points to individual nucleotides.

公开(公告)号：EP3074531A1

公开(公告)日：2016-10-05

申请号：EP14812050.4

申请日：2014-11-17

Applicant: Quantum-si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard ,  CIPRIANY, Benjamin ,  JEWELL, Jack ,  WEST, Lawrence ,  FERRIGNO, Michael ,  GLENN, Paul E. ,  COHEN, Adam E. ,  BELLOFIORE, Anthony

IPC: C12Q1/68 ,  G01N21/64 ,  G01N21/77

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

公开(公告)号：EP3068900A1

公开(公告)日：2016-09-21

申请号：EP14809194.5

申请日：2014-11-17

Applicant: Quantum-si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard

IPC: C12Q1/68

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

公开(公告)号：EP4424846A3

公开(公告)日：2024-11-20

申请号：EP24173538.0

申请日：2015-08-07

Applicant: Quantum-Si Incorporated

Inventor： ROTHBERG, Jonathan M. ,  KABIRI, Ali ,  SICKLER, Jason W. ,  GYARFAS, Brett J. ,  LACKEY, Jeremy ,  SCHMID, Gerard ,  GLENN, Paul E. ,  WEST, Lawrence C. ,  CIPRIANY, Benjamin ,  FIFE, Keith G.

IPC: G01N21/64 ,  C12Q1/6874

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.