Solution for extraction of RNA
    11.
    发明专利

    公开(公告)号:AU2011291680A1

    公开(公告)日:2013-02-28

    申请号:AU2011291680

    申请日:2011-08-17

    Abstract: Disclosed is a solution for extracting substantially pure RNA from a biological sample. A solution for extracting RNA from a biological sample containing RNA and at least DNA comprises (a) a phenol in an amount of more than 50 vol% relative to the total amount of the solution, (b) a polyhydric alcohol in an amount of 3-10 vol% relative to the total amount of the solution, (c) a guanidium salt at a concentration of 0.5-2.0 M relative to the total amount of the solution, (d) a thiocyanic acid salt at a concentration of 0.1-0.5 M relative to the total amount of the solution, and (e) a buffering agent for maintaining the solution at pH 4-6.

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