中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

24 records (took 0.047 seconds)

    A micro-bubble plate for patterning biological and non-biological materials
    11.
    发明专利
    A micro-bubble plate for patterning biological and non-biological materials 未知

    公开(公告)号：AU2006342231A1

    公开(公告)日：2007-10-25

    申请号：AU2006342231

    申请日：2006-10-10

    Applicant: UNIV CALIFORNIA

    Inventor： LI GUANN-PYNG ,  SIMS CHRISTOPHER ,  BACHMAN MARK ,  ALLBRITTON NANCY ,  WANG YULI

    IPC: C12M1/22 ,  B01L3/00

    Abstract: Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.

    FAST ELECTRICAL LYSIS OF CELLS AND RAPID COLLECTION OF THE CONTENTS THEREOF USING CAPILLARY ELECTROPHORESIS
    13.
    发明申请
    FAST ELECTRICAL LYSIS OF CELLS AND RAPID COLLECTION OF THE CONTENTS THEREOF USING CAPILLARY ELECTROPHORESIS 审中-公开
    细胞的快速电学分析和快速收集其使用毛细管电泳的内容

    公开(公告)号：WO03093791A2

    公开(公告)日：2003-11-13

    申请号：PCT/US0313599

    申请日：2003-05-02

    Applicant: UNIV CALIFORNIA

    Inventor： ALBRITTON NANCY ,  LI GUANN-PYNG ,  BACHMAN MARK ,  SIMS CHRISTOPHER ,  HAN FUTIAN

    IPC: B01L3/00 ,  G01N27/447 ,  G01N33/50 ,  G01N33/559 ,  G01N33/561 ,  G01N

    CPC classification number: G01N33/561 ,  B01L3/5027 ,  C12M47/06 ,  G01N27/44743 ,  G01N33/5005 ,  G01N33/559

    Abstract: The invention provides apparatus and methods for subsecond lysis of selected cells (58) in a cell chamber (10) using a voltage pulse of 10 ms to 10 µs in duration followed by nearly simultaneous collection of the lysed cellular contents (59) into a capillary electrophoresis tube (42) or other suitable micro-collection device (15). Cell chambers (10) and capillary electrophoresis (42) tubes configured with electrodes (18, 19) for performing the electrical lysis are described. The influence of variables that govern the rate of cell lysis, such as the inter-electrode distance, pulse duration, and pulse strength are also described. The methods are illustrated using fluorophores that are loaded into a cell (10) and then collected following electrical lysis, separated by electrophoresis, and then detected by laser-induced fluorescence detection in a capillary electrophoresis system.

    Abstract translation: 本发明提供用于使用10ms至10μs持续时间的电压脉冲在细胞室（10）中亚细胞裂解所选择的细胞（58）的装置和方法，随后裂解的细胞内含物（59）几乎同时收集到毛细管 电泳管（42）或其他合适的微量收集装置（15）。 描述了配置有用于进行电裂解的电极（18,19）的细胞室（10）和毛细管电泳（42）管。 还描述了控制细胞裂解速率的变量的影响，例如电极间距离，脉冲持续时间和脉冲强度。 使用装载到细胞中的荧光团（10），然后在电裂解后收集，通过电泳分离，然后通过毛细管电泳系统中的激光诱导荧光检测来检测方法。

    A METHOD TO MEASURE THE ACTIVATION STATE OF SIGNALING PATHWAYS IN CELLS
    14.
    发明申请
    A METHOD TO MEASURE THE ACTIVATION STATE OF SIGNALING PATHWAYS IN CELLS 审中-公开
    一种测量细胞内信号通路活化状态的方法

    公开(公告)号：WO0242732A3

    公开(公告)日：2002-08-01

    申请号：PCT/US0143966

    申请日：2001-11-21

    Applicant: UNIV CALIFORNIA ,  ALLBRITTON NANCY L ,  SIMS CHRISTOPHER

    Inventor： ALLBRITTON NANCY L ,  SIMS CHRISTOPHER

    IPC: B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/42 ,  C12Q1/02 ,  G01N1/02 ,  G01N15/14 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  G01N33/48

    CPC classification number: G01N33/6803 ,  B01L3/5027 ,  G01N1/02 ,  G01N15/1475 ,  G01N33/5005

    Abstract: The activity of multiple proteins in a single living cell, portion of a cell or in a group of cells is simultaneously measured by introducing reporter molecules. The reporter(s) is chemically modified by the enzyme of interest. In some cases the enzyme(s) is affected by the addition of a stimulus or a pharmaceutical compound to the cell. The reactions between the enzymes and the reporters are diminished or terminated, and the reporter and modified reporter are removed. The activity of the enzyme(s) is determined by measuring the amount of reporter remaining, the amount of altered reporter produced, or by comparing the amount of reporter to the amount of altered reporter. A database is compiled of the activities of the different proteins. By performing a series of experiments at different time points, conditions, and varieties of cell types, a database is developed for molecular cellular mechanisms in health and disease states. By exposing cells to variety of compounds data for drug development and screening is provided.

    Abstract translation: 通过引入报道分子同时测量单个活细胞，细胞的一部分或一组细胞中的多种蛋白质的活性。 报道分子被感兴趣的酶化学修饰。 在一些情况下，酶（一种或多种）受到刺激或药物化合物加入细胞的影响。 酶和报道分子之间的反应被减弱或终止，并且报道子和修饰的报道分子被去除。 酶的活性通过测量剩余的报告物的量，产生的改变的报告物的量或通过比较报道物的量与改变的报道物的量来确定。 数据库汇编了不同蛋白质的活性。 通过在不同时间点，条件和不同类型的细胞中进行一系列实验，为健康和疾病状态下的分子细胞机制开发了一个数据库。 通过将细胞暴露于各种化合物，提供了用于药物开发和筛选的数据。

    MICROPATTERNED PLATE WITH MICRO-PALLETS FOR ADDRESSABLE BIOCHEMICAL ANALYSIS
    15.
    发明专利
    MICROPATTERNED PLATE WITH MICRO-PALLETS FOR ADDRESSABLE BIOCHEMICAL ANALYSIS 未知

    公开(公告)号：CA2580853C

    公开(公告)日：2014-04-29

    申请号：CA2580853

    申请日：2005-10-04

    Applicant: UNIV CALIFORNIA

    Inventor： BACHMAN MARK ,  LI GUANN-PYNG ,  ALLBRITTON NANCY ,  SIMS CHRISTOPHER

    IPC: C12Q1/68 ,  C12M1/34

    Abstract: A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.

    A micro-bubble plate for patterning biological and non-biological materials
    17.
    发明专利
    A micro-bubble plate for patterning biological and non-biological materials 未知

    公开(公告)号：AU2006342231B2

    公开(公告)日：2012-04-19

    申请号：AU2006342231

    申请日：2006-10-10

    Applicant: UNIV CALIFORNIA

    Inventor： LI GUANN-PYNG ,  SIMS CHRISTOPHER ,  BACHMAN MARK ,  ALLBRITTON NANCY ,  WANG YULI

    IPC: B01L3/00 ,  C12M1/22

    Abstract: Systems and methods for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiments of the present invention include a method for placing materials on the plate at predetermined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures where the structures may be formed from living or nonliving material, organic or inorganic, and the like.

    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS
    20.
    发明专利
    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 未知

    公开(公告)号：CA2625071A1

    公开(公告)日：2007-10-25

    申请号：CA2625071

    申请日：2006-10-10

    Applicant: UNIV CALIFORNIA

    Inventor： ALLBRITTON NANCY ,  SIMS CHRISTOPHER ,  LI GUANN-PYNG ,  WANG YULI ,  BACHMAN MARK

    IPC: C12M1/22 ,  B01L3/00

    Abstract: Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.

Patent Agency Ranking