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    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS
    2.
    发明申请
    A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 审中-公开
    用于绘制生物和非生物材料的微孔板

    公开(公告)号：WO2007120183A3

    公开(公告)日：2008-01-10

    申请号：PCT/US2006039929

    申请日：2006-10-10

    Applicant: UNIV CALIFORNIA ,  WANG YULI ,  BACHMAN MARK ,  SIMS CHRISTOPHER ,  LI GUANN-PYNG ,  ALLBRITTON NANCY

    Inventor： WANG YULI ,  BACHMAN MARK ,  SIMS CHRISTOPHER ,  LI GUANN-PYNG ,  ALLBRITTON NANCY

    IPC: C12M1/22 ,  B01L3/00

    CPC classification number: B01L3/5085 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0819 ,  C12M25/06 ,  C12M29/20

    Abstract: Systems and methods for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate (6) with regions (16) that will trap gas, usually in the form of bubbles (10), when the plate (6) is submerged in liquid. Other embodiments of the present invention include a method for placing materials on the plate (6) at predetermined locations through the use of trapped gas to prevent materials from collecting at unwante regions. The plate (6) has great utility for plating cells and tissues (5) at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate (6) at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures where the structures may be formed from living or nonliving material, organic or inorganic, and the like.

    Abstract translation: 用于在板上的特定位置图案化生物和非生物材料的系统和方法，以及生长三维结构。 优选实施例包括具有区域（16）的板（6），当板（6）浸没在液体中时，该板（6）将通常以气泡（10）的形式捕获气体。 本发明的其他实施例包括通过使用被捕获的气体将材料放置在板（6）上的预定位置处的方法，以防止材料在不透水区域收集。 板（6）对于在特定位置（例如阵列）上的电镀细胞和组织（5）具有很大的用途。 所公开的方法还可以用于在特定位置涂覆板（6）的表面用于图案化涂覆应用，并且建立材料以产生三维结构，包括微机械结构，其中结构可以由活的或非活性的材料形成， 有机或无机等。

    DETECTION OF CELLS USING MOLECULAR ELECTROPHORETIC CHANGE
    5.
    发明申请
    DETECTION OF CELLS USING MOLECULAR ELECTROPHORETIC CHANGE 审中-公开
    使用分子电泳改变检测细胞

    公开(公告)号：WO02092199A8

    公开(公告)日：2002-12-27

    申请号：PCT/US0214755

    申请日：2002-05-09

    Applicant: UNIV CALIFORNIA

    Inventor： ALLBRITTON NANCY ,  SIMS CHRISTOPHER

    IPC: G01N27/62 ,  B01D53/62 ,  B01D57/02 ,  B03C5/00 ,  C08F2/58 ,  C12M1/26 ,  C12M1/34 ,  C12M1/42 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/48 ,  G01N27/447 ,  G01N27/48 ,  G01N33/483 ,  G01N33/50 ,  B01D59/42 ,  B01D59/50 ,  B01D61/42 ,  B01D61/58 ,  C02F1/26 ,  C25B7/00 ,  C25B15/00 ,  G01F1/64 ,  G01L1/20 ,  G01L9/18 ,  G01N27/26

    CPC classification number: C12Q1/00 ,  B01D53/62 ,  C12Q1/485 ,  G01N27/44721 ,  G01N27/44743 ,  G01N33/50 ,  G01N33/5017

    Abstract: The activity of encogenic intercellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules that underdo a change in electrophoretic mobility upon a chemical reaction such as that catalyzed by an enzyme or kinase. Specificity is achieved by using labeled substrate molecules that can be acted upon only by specific oncogenic enzymes can be determined. Measurements are made with the intercellular presence of such substrate molecules, at some time of interest. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occuring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of said cell or cells into which reporter substrate molecules have been introduced. The cell contents are then subjected to capillary electrophoresis and oncogenic enzymatic activity is determined by comparing amounts of unaltered substrate molecules to the amounts of altered substrate molecules which are separated by the electrophoresis and identified by the presence of a fluorescent label.

    Abstract translation: 通过使用荧光标记的底物分子来测量分子的细胞间化学反应的活性，所述底物分子在诸如由酶或激酶催化的化学反应引起的电泳迁移率发生变化。 通过使用可以仅通过特异性致癌酶作用的标记的底物分子来实现特异性。 在感兴趣的某个时间，用这种底物分子的细胞间存在进行测量。 为了确保准确性，必须及时进行测量，以尽可能减少感兴趣的时间后发生的化学反应。 使用快速可控的激光裂解来获得引入了报告基质分子的所述细胞或细胞的内容物。 然后对细胞内容物进行毛细管电泳，并且通过将未改变的底物分子的量与通过电泳分离并通过荧光标记的存在鉴定的改变的底物分子的量进行比较来确定致癌酶活性。

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