公开(公告)号：CA2648778A1

公开(公告)日：2007-10-18

申请号：CA2648778

申请日：2007-04-06

Applicant: UNIV CALIFORNIA

Inventor： LI GUANN-PYNG ,  SIMS CHRISTOPHER E ,  BACHMAN MARK ,  WANG YULI ,  ALLBRITTON NANCY ,  STANBRIDGE ERIC

IPC: C12Q1/24 ,  C12M1/26 ,  C12Q1/04 ,  C12Q1/68 ,  C40B20/00 ,  C40B30/00

Abstract: A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. T he plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.

公开(公告)号：IS5608A

公开(公告)日：2000-08-29

申请号：IS5608

申请日：2000-08-29

Applicant: UNIV CALIFORNIA

Inventor： ALLBRITTON NANCY L ,  SIMS CHRISTOPHER E ,  BERNS MICHAEL W ,  MEREDITH GAVIN D ,  KRASIEVA TATIANA B ,  TROMBERG BRUCE J

IPC: G01N33/48 ,  C12M1/33 ,  C12N13/00 ,  G01N27/447 ,  G01N33/483 ,  G01N27/26

公开(公告)号：CA2322857A1

公开(公告)日：1999-09-10

申请号：CA2322857

申请日：1999-03-03

Applicant: UNIV CALIFORNIA

Inventor： TROMBERG BRUCE J ,  BERNS MICHAEL W ,  SIMS CHRISTOPHER E ,  ALLBRITTON NANCY L ,  KRASIEVA TATIANA B ,  MEREDITH GAVIN D

IPC: G01N33/48 ,  C12M1/33 ,  C12N13/00 ,  G01N27/447 ,  G01N33/483 ,  C12M3/00 ,  G01N27/26 ,  C12M1/42

Abstract: Fast lysis of a single cell (46) or cellular component thereof is performed by generating a shock wave in a medium in which the cell (46) or cellular component thereof is positioned. The cell (46) or cellular component thereof is either positioned by laser tweezers or cultured as an adhered cell or cellular component thereof to minimize manipulation trauma. The disclosed method completely lyses a single cell (46) or cellular component thereof in a controllable manner in milliseconds or less followed immediately by the loading of the cellular contents into a capillary (22) for analyte separation and detection. The cell (46) or cellular component thereof is adjacent the inlet of an electrophoretic column (22) through which a gravity siphon flow of the medium is maintained. The lysed contents of the cell (46) or cellular component thereof enter the electrophoretic column (22) in less than 33 msec, and are separated and analyzed by a fluorescence detector (42). The method takes advantage of the shock wave produced by a highly focused laser pulse from a Nd:YAG laser (18) which is created in a medium adjacent to the cell (46) or cellular component thereof. In the illustrated embodiment, the laser pulse is focused in the slide (28) at or near a glass-to-buffer interface of a cell chamber in which the cell (46) or cellular component thereof to be lysed has been cultured.